Abstract: The present technology relates to compositions and methods for modulating expression of genes which include a target oligonucleotide sequence, such as repeats of a particular oligonucleotide sequence containing 3 to 10 nucleotides. In particular aspects, the present technology relates to agents having a formula A-L-B, wherein -L- is a linker; A- is a Brd4 binding moiety; and —B is a nucleic acid binding moiety, such as a polyamide or complementary oligonucleotide, that specifically binds to the target oligonucleotide sequence.

Abstract: The present technology relates to compositions and methods for modulating expression of genes, which include a target oligonucleotide sequence, such as repeats of a particular oligonucleotide sequence containing 3 to 10 nucleotides. In particular aspects, the present technology relates to agents having a formula A-L-B, wherein -L- is a linker; A- is a Brd4 binding moiety; and -B is a nucleic acid binding moiety, such as a polyamide or complementary oligonucleotide, that specifically binds to the target oligonucleotide sequence.

Abstract: A method of evaluating one or more test compounds to identify test compounds that modulate binding of natural or artificial regulatory factors to corresponding single-, double-, or triple-stranded nucleic acid binding sites is described. The method utilizes an isolated nucleic acid target that defines at least one known or putative binding site for a regulatory factor. The nucleic acid target has conjugated or covalently bonded thereto, at a point proximate to, but not within, the binding site: (i) an anchor moiety; (ii) a linker moiety bonded to the anchor moiety; and (iii) a test compound bonded to the linker moiety. To evaluate the test compound, the nucleic acid target of step is then contacted to a reagent mixture comprising one or more natural or artificial regulatory factors specific for the binding site defined in the nucleic acid target.