Abstract: The present invention relates to artificial transcription factors (ATFs) that alter gene expression, including inducing pluripotency in cells or promoting the conversion of cells to specific cell fates. In particular, provided herein is a zinc-finger based ATF library that can be screened in cells by looking for expression of a specific gene (e.g., reporter expression), monitoring for cell surface markers or morphology, or via functional assays.

Abstract: The present invention relates to artificial transcription factors (ATFs) that alter gene expression, including inducing pluripotency in cells or promoting the conversion of cells to specific cell fates. In particular, provided herein is a zinc-finger based ATF library that can be screened in cells by looking for expression of a specific gene (e.g., reporter expression), monitoring for cell surface markers or morphology, or via functional assays.

Abstract: The present invention relates to artificial transcription factors (ATFs) that alter gene expression, including inducing pluripotency in cells or promoting the conversion of cells to specific cell fates. In particular, provided herein is a zinc-finger based ATF library that can be screened in cells by looking for expression of a specific gene (e.g., reporter expression), monitoring for cell surface markers or morphology, or via functional assays.

Abstract: Synthetic transcriptions factors for modulation of frataxin expression and methods of use are described herein.

Abstract: The present invention relates to artificial transcription factors (ATFs) that alter gene expression, including inducing pluripotency in cells or promoting the conversion of cells to specific cell fates. In particular, provided herein is a zinc-finger based ATF library that can be screened in cells by looking for expression of a specific gene (e.g., reporter expression), monitoring for cell surface markers or morphology, or via functional assays.

Abstract: A system and method for analyzing DNA binding specificities is provided. The potential binding motifs are compared to a plurality of DNA sequences. The DNA sequences are plotted within a specificity landscape, which provides details otherwise unavailable, relating to binding affinities and binding specificities of the motif-sequence combination.

Abstract: A system and method for analyzing DNA binding specificities is provided. The potential binding motifs are compared to a plurality of DNA sequences. The DNA sequences are plotted within a specificity landscape, which provides details otherwise unavailable, relating to binding affinities and binding specificities of the motif-sequence combination.

Abstract: The invention provides reliable, reusable materials, system and methods for use in nucleic acid binding assays between a nucleic-acid binding assay molecule and a robust unimolecular target. In a preferred embodiment, the target is a nucleic acid-containing molecule, L1-X1-L2-X2 (where L1 is the linker to the solid support, and L2 is the linker between the two nucleotide-pairing regions, X1 and X2; L2 folds such that double-stranded DNA structure forms between X1 and X2). L2 is a polynucleotide three nucleotides in length, having the sequence GNA, where N is A, G, C, T, or U. A typical assay uses a target array, where multiple targets are bound to a solid surface. The linker, L1, of the nucleic acid target to the supporting surface is useful although it is significantly longer than suggested in the art. The length of L1 is preferably 8 to 30 nucleotides in length, more preferably from 10 to 20 nucleotides.