Patents by Inventor Ashraf Wahba
Ashraf Wahba has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11891661Abstract: A method of detecting a lipid bilayer formed in a cell of a nanopore based sequencing chip is disclosed. An integrating capacitor is coupled with a lipid membrane, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to a change in the AC voltage is determined. Whether the lipid membrane comprises a lipid bilayer is detected based on the determined change in the sampled voltage across the integrating capacitor in response to the change in the AC voltage.Type: GrantFiled: December 20, 2022Date of Patent: February 6, 2024Assignee: Roche Sequencing Solutions, Inc.Inventors: Kevin Aliado, Roger J. A. Chen, Jing Luo, William Nielsen, Kyle Umeda, Ashraf Wahba
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Publication number: 20240035083Abstract: A method of forming a plurality of lipid bilayers over an array of cells in a nanopore based sequencing chip is disclosed. Each of the cells comprises a well. A salt buffer solution is flowed over the array of cells in the nanopore based sequencing chip to substantially fill the wells in the cells with the salt buffer solution. A lipid and solvent mixture is flowed over the array of cells to deposit the lipid and solvent mixture over at least some of the wells in the cells. A first portion of the cells, each having a lipid bilayer over its well, is detected. A second portion of the cells, each having a lipid membrane but not a lipid bilayer over its well, is detected. An electrical lipid-thinning stimulus is selectively applied to the second portion of the cells but not to the first portion of the cells.Type: ApplicationFiled: October 6, 2023Publication date: February 1, 2024Inventors: Kevin Aliado, Roger J.A. Chen, Jing Luo, J. William Maney, JR., William Nielsen, Kyle Umeda, Ashraf Wahba
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Patent number: 11814676Abstract: A method of forming a plurality of lipid bilayers over an array of cells in a nanopore based sequencing chip is disclosed. Each of the cells comprises a well. A salt buffer solution is flowed over the array of cells in the nanopore based sequencing chip to substantially fill the wells in the cells with the salt buffer solution. A lipid and solvent mixture is flowed over the array of cells to deposit the lipid and solvent mixture over at least some of the wells in the cells. A first portion of the cells, each having a lipid bilayer over its well, is detected. A second portion of the cells, each having a lipid membrane but not a lipid bilayer over its well, is detected. An electrical lipid-thinning stimulus is selectively applied to the second portion of the cells but not to the first portion of the cells.Type: GrantFiled: June 21, 2021Date of Patent: November 14, 2023Assignee: Roche Sequencing Solutions, Inc.Inventors: Kevin Aliado, Roger J. A. Chen, Jing Luo, J. William Maney, Jr., William Nielsen, Kyle Umeda, Ashraf Wahba
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Publication number: 20230332204Abstract: A method of detecting a state of a lipid membrane in a cell of a nanopore based sequencing chip is disclosed. A lipid membrane is coupled with an integrating capacitor, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to an intermediate change in the AC voltage is determined. A state of the lipid membrane is determined based on the determined change in the sampled voltage across the integrating capacitor in response to the intermediate change in the AC voltage.Type: ApplicationFiled: June 23, 2023Publication date: October 19, 2023Inventor: Ashraf Wahba
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Patent number: 11725229Abstract: A method of detecting a state of a lipid membrane in a cell of a nanopore based sequencing chip is disclosed. A lipid membrane is coupled with an integrating capacitor, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to an intermediate change in the AC voltage is determined. A state of the lipid membrane is determined based on the determined change in the sampled voltage across the integrating capacitor in response to the intermediate change in the AC voltage.Type: GrantFiled: April 7, 2020Date of Patent: August 15, 2023Assignee: Roche Sequencing Solutions, Inc.Inventor: Ashraf Wahba
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Publication number: 20230123791Abstract: A curing device is positionable to a mount on a resin injector to cure the resin injected into a defect in glass. A housing of the curing device is removably positionable to a mounted position on the resin injector. When in the mounted position the curing device positions a light area from light emitters to an area surrounding the nozzle of the injector to provide curing of the injected resin. A GPS locator can ascertain a location, date, and time of use of the curing device and store such use information in electronic memory.Type: ApplicationFiled: October 19, 2022Publication date: April 20, 2023Inventors: Shiloh Judah Spoo, Dominik Scheffler, Ashraf Wahba
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Publication number: 20230120047Abstract: A method of detecting a lipid bilayer formed in a cell of a nanopore based sequencing chip is disclosed. An integrating capacitor is coupled with a lipid membrane, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to a change in the AC voltage is determined. Whether the lipid membrane comprises a lipid bilayer is detected based on the determined change in the sampled voltage across the integrating capacitor in response to the change in the AC voltage.Type: ApplicationFiled: December 20, 2022Publication date: April 20, 2023Inventors: Kevin Aliado, Roger J.A. Chen, Jing Luo, William Nielsen, Kyle Umeda, Ashraf Wahba
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Patent number: 11530443Abstract: A method of detecting a lipid bilayer formed in a cell of a nanopore based sequencing chip is disclosed. An integrating capacitor is coupled with a lipid membrane, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to a change in the AC voltage is determined. Whether the lipid membrane comprises a lipid bilayer is detected based on the determined change in the sampled voltage across the integrating capacitor in response to the change in the AC voltage.Type: GrantFiled: June 3, 2020Date of Patent: December 20, 2022Assignee: Roche Sequencing Solutions, Inc.Inventors: Kevin Aliado, Roger J. A. Chen, Jing Luo, William Nielsen, Kyle Umeda, Ashraf Wahba
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Publication number: 20220090183Abstract: Embodiments relate to methods and compositions useful for routing and tracking multiple mobile units within a microfluidic device. Mobile units may be routed through a plurality of chemical environments, and the mobile units may be tracked to determine the path and/or environments that the mobile units have routed through. Mobile units may be routed in accordance with a predetermined algorithm. Mobile units may be routed through microfluidic devices in ordered flow. Mobile units routed through the microfluidic device can be used to perform various chemical reactions uniquely associated to the units, including without limitation peptide synthesis, enzymatic gene synthesis and gene assembly.Type: ApplicationFiled: February 25, 2020Publication date: March 24, 2022Inventors: Matthew HILL, Marc UNGER, Ashraf WAHBA, Ouriel E. CAEN
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Publication number: 20210310064Abstract: A method of forming a plurality of lipid bilayers over an array of cells in a nanopore based sequencing chip is disclosed. Each of the cells comprises a well. A salt buffer solution is flowed over the array of cells in the nanopore based sequencing chip to substantially fill the wells in the cells with the salt buffer solution. A lipid and solvent mixture is flowed over the array of cells to deposit the lipid and solvent mixture over at least some of the wells in the cells. A first portion of the cells, each having a lipid bilayer over its well, is detected. A second portion of the cells, each having a lipid membrane but not a lipid bilayer over its well, is detected. An electrical lipid-thinning stimulus is selectively applied to the second portion of the cells but not to the first portion of the cells.Type: ApplicationFiled: June 21, 2021Publication date: October 7, 2021Inventors: Kevin Aliado, Roger J.A. Chen, Jing Luo, J. William Maney, JR., William Nielsen, Kyle Umeda, Ashraf Wahba
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Patent number: 11041198Abstract: A method of forming a plurality of lipid bilayers over an array of cells in a nanopore based sequencing chip is disclosed. Each of the cells comprises a well. A salt buffer solution is flowed over the array of cells in the nanopore based sequencing chip to substantially fill the wells in the cells with the salt buffer solution. A lipid and solvent mixture is flowed over the array of cells to deposit the lipid and solvent mixture over at least some of the wells in the cells. A first portion of the cells, each having a lipid bilayer over its well, is detected. A second portion of the cells, each having a lipid membrane but not a lipid bilayer over its well, is detected. An electrical lipid-thinning stimulus is selectively applied to the second portion of the cells but not to the first portion of the cells.Type: GrantFiled: September 20, 2019Date of Patent: June 22, 2021Assignee: Roche Sequencing Solutions, Inc.Inventors: Kevin Aliado, Roger J. A. Chen, Jing Luo, J. William Maney, Jr., William Nielsen, Kyle Umeda, Ashraf Wahba
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Publication number: 20200291468Abstract: A method of detecting a lipid bilayer formed in a cell of a nanopore based sequencing chip is disclosed. An integrating capacitor is coupled with a lipid membrane, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to a change in the AC voltage is determined. Whether the lipid membrane comprises a lipid bilayer is detected based on the determined change in the sampled voltage across the integrating capacitor in response to the change in the AC voltage.Type: ApplicationFiled: June 3, 2020Publication date: September 17, 2020Inventors: Kevin Aliado, Roger J.A. Chen, Jing Luo, William Nielsen, Kyle Umeda, Ashraf Wahba
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Publication number: 20200232008Abstract: A method of detecting a state of a lipid membrane in a cell of a nanopore based sequencing chip is disclosed. A lipid membrane is coupled with an integrating capacitor, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to an intermediate change in the AC voltage is determined. A state of the lipid membrane is determined based on the determined change in the sampled voltage across the integrating capacitor in response to the intermediate change in the AC voltage.Type: ApplicationFiled: April 7, 2020Publication date: July 23, 2020Inventor: Ashraf Wahba
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Patent number: 10683543Abstract: A method of detecting a lipid bilayer formed in a cell of a nanopore based sequencing chip is disclosed. An integrating capacitor is coupled with a lipid membrane, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to a change in the AC voltage is determined. Whether the lipid membrane comprises a lipid bilayer is detected based on the determined change in the sampled voltage across the integrating capacitor in response to the change in the AC voltage.Type: GrantFiled: November 12, 2018Date of Patent: June 16, 2020Assignee: Roche Molecular Systems, Inc.Inventors: Ashraf Wahba, William Nielsen, Jing Luo, Kevin Aliado, Kyle Umeda, Roger J.A. Chen
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Patent number: 10648016Abstract: A method of detecting a state of a lipid membrane in a cell of a nanopore based sequencing chip is disclosed. A lipid membrane is coupled with an integrating capacitor, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to an intermediate change in the AC voltage is determined. A state of the lipid membrane is determined based on the determined change in the sampled voltage across the integrating capacitor in response to the intermediate change in the AC voltage.Type: GrantFiled: January 29, 2019Date of Patent: May 12, 2020Assignee: Roche Sequencing Solutions, Inc.Inventor: Ashraf Wahba
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Publication number: 20200032333Abstract: A method of forming a plurality of lipid bilayers over an array of cells in a nanopore based sequencing chip is disclosed. Each of the cells comprises a well. A salt buffer solution is flowed over the array of cells in the nanopore based sequencing chip to substantially fill the wells in the cells with the salt buffer solution. A lipid and solvent mixture is flowed over the array of cells to deposit the lipid and solvent mixture over at least some of the wells in the cells. A first portion of the cells, each having a lipid bilayer over its well, is detected. A second portion of the cells, each having a lipid membrane but not a lipid bilayer over its well, is detected. An electrical lipid-thinning stimulus is selectively applied to the second portion of the cells but not to the first portion of the cells.Type: ApplicationFiled: September 20, 2019Publication date: January 30, 2020Inventors: Kevin Aliado, Roger J.A. Chen, Jing Luo, J. William Maney, JR., William Nielsen, Kyle Umeda, Ashraf Wahba
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Patent number: 10465240Abstract: A method of forming a plurality of lipid bilayers over an array of cells in a nanopore based sequencing chip is disclosed. Each of the cells comprises a well. A salt buffer solution is flowed over the array of cells in the nanopore based sequencing chip to substantially fill the wells in the cells with the salt buffer solution. A lipid and solvent mixture is flowed over the array of cells to deposit the lipid and solvent mixture over at least some of the wells in the cells. A first portion of the cells, each having a lipid bilayer over its well, is detected. A second portion of the cells, each having a lipid membrane but not a lipid bilayer over its well, is detected. An electrical lipid-thinning stimulus is selectively applied to the second portion of the cells but not to the first portion of the cells.Type: GrantFiled: March 30, 2016Date of Patent: November 5, 2019Assignee: Roche Sequencing Solutions, Inc.Inventors: Ashraf Wahba, William Nielsen, J. William Maney, Jr., Kevin Aliado, Kyle Umeda, Roger J. A. Chen, Jing Luo
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Publication number: 20190153506Abstract: A method of detecting a state of a lipid membrane in a cell of a nanopore based sequencing chip is disclosed. A lipid membrane is coupled with an integrating capacitor, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to an intermediate change in the AC voltage is determined. A state of the lipid membrane is determined based on the determined change in the sampled voltage across the integrating capacitor in response to the intermediate change in the AC voltage.Type: ApplicationFiled: January 29, 2019Publication date: May 23, 2019Inventor: Ashraf Wahba
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Publication number: 20190144934Abstract: A method of detecting a lipid bilayer formed in a cell of a nanopore based sequencing chip is disclosed. An integrating capacitor is coupled with a lipid membrane, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to a change in the AC voltage is determined. Whether the lipid membrane comprises a lipid bilayer is detected based on the determined change in the sampled voltage across the integrating capacitor in response to the change in the AC voltage.Type: ApplicationFiled: November 12, 2018Publication date: May 16, 2019Inventors: Ashraf Wahba, William Nielsen, Jing Luo, Kevin Aliado, Kyle Umeda, Roger J. A. Chen
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Patent number: 10233486Abstract: A method of detecting a state of a lipid membrane in a cell of a nanopore based sequencing chip is disclosed. A lipid membrane is coupled with an integrating capacitor, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to an intermediate change in the AC voltage is determined. A state of the lipid membrane is determined based on the determined change in the sampled voltage across the integrating capacitor in response to the intermediate change in the AC voltage.Type: GrantFiled: September 26, 2017Date of Patent: March 19, 2019Assignee: Roche Sequencing Solutions, Inc.Inventor: Ashraf Wahba