Patents by Inventor Asmamaw Wassie
Asmamaw Wassie has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 11971417Abstract: The present description provides methods, assays and reagents useful for sequencing proteins. Sequencing proteins in a broad sense involves observing the plausible identity and order of amino acids, which is useful for sequencing single polypeptide molecules or multiple molecules of a single polypeptide. In one aspect, the methods are useful for sequencing multiple polypeptides. The methods and reagents described herein can be useful for high resolution interrogation of the proteome and enabling ultrasensitive diagnostics critical for early detection of diseases.Type: GrantFiled: October 12, 2022Date of Patent: April 30, 2024Assignee: Massachusetts Institute of TechnologyInventors: Daniel Masao Estandian, Alexi Georges Choueiri, Edward Stuart Boyden, Asmamaw Wassie
-
Publication number: 20240076752Abstract: The invention provides methods, kits, and related compositions for rapid, low-cost, point of care detection of an organism, such as a virus or bacteria, in a biological or environmental sample using isothermal nucleic acid amplification.Type: ApplicationFiled: January 5, 2022Publication date: March 7, 2024Inventors: Fahim Farzadfard, Asmamaw Wassie, Jeong Seuk Kang, Ho-Jun Suk, Kayla Vodehnal, Justin Liu, Jianghong Min
-
Publication number: 20230305017Abstract: The present description provides methods, assays and reagents useful for sequencing proteins. Sequencing proteins in a broad sense involves observing the plausible identity and order of amino acids, which is useful for sequencing single polypeptide molecules or multiple molecules of a single polypeptide. In one aspect, the methods are useful for sequencing multiple polypeptides. The methods and reagents described herein can be useful for high resolution interrogation of the proteome and enabling ultrasensitive diagnostics critical for early detection of diseases.Type: ApplicationFiled: October 12, 2022Publication date: September 28, 2023Inventors: Daniel Masao Estandian, Alexi Georges Choueiri, Edward Stuart Boyden, Asmamaw Wassie
-
Publication number: 20230193245Abstract: This disclosure provides methods and compositions for making and using a protein or peptide array.Type: ApplicationFiled: June 29, 2020Publication date: June 22, 2023Inventors: Asmamaw Wassie, Annalisa Marie Pawlosky, Mariya Chavarha, Phillip Jess, Marc Berndl
-
Patent number: 11499979Abstract: The present description provides methods, assays and reagents useful for sequencing proteins. Sequencing proteins in a broad sense involves observing the plausible identity and order of amino acids, which is useful for sequencing single polypeptide molecules or multiple molecules of a single polypeptide. In one aspect, the methods are useful for sequencing multiple polypeptides. The methods and reagents described herein can be useful for high resolution interrogation of the proteome and enabling ultrasensitive diagnostics critical for early detection of diseases.Type: GrantFiled: January 7, 2020Date of Patent: November 15, 2022Assignee: Massachusetts Institute of TechnologyInventors: Daniel Masao Estandian, Alexi Georges Choueiri, Edward Stuart Boyden, Asmamaw Wassie
-
Publication number: 20210395796Abstract: The present invention relates to a method for amplifying at least one target RNA in a fixed and, optionally, expanded biological sample. In an embodiment of the invention, the method comprises incubating the fixed biological sample with a pair of polynucleotides complementary to non-overlapping and proximal sequences of a target RNA, wherein the polynucleotide pair hybridizes to the target RNA; ligating the polynucleotide pair using a ligase; and amplifying the ligation product. The invention further provides methods for detecting and optionally quantifying and/or sequencing the amplification product. As the method comprises hybridizing polynucleotide pairs to a target RNA in a fixed biological sample, the target RNA can be hybridized in situ.Type: ApplicationFiled: April 30, 2021Publication date: December 23, 2021Inventors: Fei Chen, Asmamaw Wassie, Shahar Alon, Adam Henry Marblestone, Anubhav Sinha, Andrew C. Payne, Edward Stuart Boyden
-
Publication number: 20200217853Abstract: The present description provides methods, assays and reagents useful for sequencing proteins. Sequencing proteins in a broad sense involves observing the plausible identity and order of amino acids, which is useful for sequencing single polypeptide molecules or multiple molecules of a single polypeptide. In one aspect, the methods are useful for sequencing multiple polypeptides. The methods and reagents described herein can be useful for high resolution interrogation of the proteome and enabling ultrasensitive diagnostics critical for early detection of diseases.Type: ApplicationFiled: January 7, 2020Publication date: July 9, 2020Inventors: Daniel Masao Estandian, Alexi Georges Choueiri, Edward Stuart Boyden, Asmamaw Wassie
-
Patent number: 10545145Abstract: The invention, in some aspects relates to compositions and methods for imaging biological systems and physiological activity and conditions in cells.Type: GrantFiled: April 14, 2016Date of Patent: January 28, 2020Assignee: Massachusetts Institute of TechnologyInventors: Or Shemesh, Asmamaw Wassie, Chih-Chieh Yu, Edward Boyden
-
Patent number: 10526649Abstract: The invention provides in situ nucleic acid sequencing to be conducted in biological specimens that have been physically expanded. The invention leverages the techniques for expansion microscopy (ExM) to provide new methods for in situ sequencing of nucleic acids in a process referred to herein as “expansion sequencing” (ExSEQ).Type: GrantFiled: October 20, 2017Date of Patent: January 7, 2020Assignees: Massachusetts Institute of Technology, President and Fellows of Harvard CollegeInventors: Fei Chen, Shahar Alon, Andrew Payne, Asmamaw Wassie, Daniel Goodwin, Edward Stuart Boyden, Evan Daugharthy, Jonathan Scheiman
-
Patent number: 10364457Abstract: The invention enables in situ genomic and transcriptomic assessment of nucleic acids to be conducted in biological specimens that have been physically expanded. The invention leverages the techniques for expansion microscopy (ExM) to provide new methods for in situ genomic and transcriptomic assessment of nucleic in a new process referred to herein as “expansion fluorescent in situ hybridization” (ExFISH).Type: GrantFiled: August 5, 2016Date of Patent: July 30, 2019Assignee: Massachusetts Institute of TechnologyInventors: Asmamaw Wassie, Fei Chen, Edward Stuart Boyden, Shahar Alon, George Church, Evan Daugharthy
-
Publication number: 20180119219Abstract: The invention provides in situ nucleic acid sequencing to be conducted in biological specimens that have been physically expanded. The invention leverages the techniques for expansion microscopy (ExM) to provide new methods for in situ sequencing of nucleic acids in a process referred to herein as “expansion sequencing” (ExSEQ).Type: ApplicationFiled: October 20, 2017Publication date: May 3, 2018Inventors: Fei Chen, Shahar Alon, Andrew Payne, Asmamaw Wassie, Daniel Goodwin, Edward Stuart Boyden
-
Publication number: 20170067096Abstract: The invention enables in situ genomic and transcriptomic assessment of nucleic acids to be conducted in biological specimens that have been physically expanded. The invention leverages the techniques for expansion microscopy (ExM) to provide new methods for in situ genomic and transcriptomic assessment of nucleic in a new process referred to herein as “expansion fluorescent in situ hybridization” (ExFISH).Type: ApplicationFiled: August 5, 2016Publication date: March 9, 2017Inventors: Asmamaw Wassie, Fei Chen, Edward Stuart Boyden, Shahar Alon
-
Publication number: 20160305939Abstract: The invention, in some aspects relates to compositions and methods for imaging biological systems and physiological activity and conditions in cells.Type: ApplicationFiled: April 14, 2016Publication date: October 20, 2016Applicant: Massachusetts Institute of TechnologyInventors: Or Shemesh, Asmamaw Wassie, Chih-Chieh Yu, Edward Boyden
-
Patent number: 9377447Abstract: In exemplary implementations of this invention, an electronic olfactor determines whether a scent being tested matches the scent of a positive control. The electronic olfactor can perform this scent matching even in a changing olfactory environment, and even if the positive control scent is a combination of hundreds or thousands of different odorants. No prior training is needed, and no attempt is made to identify a single odorant that is unambiguously responsible for a scent. Instead, a computer compares the total scent pattern of a positive control sample with the total scent pattern of a test sample, across a sweep of many permutations of electrical inputs to scent sensors, to try to find any condition under which the total scent patterns do not match. If such a condition cannot be found, then the computer declares a match between the test and target scents.Type: GrantFiled: July 31, 2015Date of Patent: June 28, 2016Assignee: Massachusetts Institute of TechnologyInventors: Andreas Mershin, Asmamaw Wassie, Yael Maguire, David Kong, Shuguang Zhang, Patrick Moran, Karolina Corin
-
Publication number: 20150362469Abstract: In exemplary implementations of this invention, an electronic olfactor determines whether a scent being tested matches the scent of a positive control. The electronic olfactor can perform this scent matching even in a changing olfactory environment, and even if the positive control scent is a combination of hundreds or thousands of different odorants. No prior training is needed, and no attempt is made to identify a single odorant that is unambiguously responsible for a scent. Instead, a computer compares the total scent pattern of a positive control sample with the total scent pattern of a test sample, across a sweep of many permutations of electrical inputs to scent sensors, to try to find any condition under which the total scent patterns do not match. If such a condition cannot be found, then the computer declares a match between the test and target scents.Type: ApplicationFiled: July 31, 2015Publication date: December 17, 2015Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGYInventors: Andreas Mershin, Asmamaw Wassie, Yael Maguire, David Kong, Shuguang Zhang, Patrick Moran, Karolina Corin
-
Patent number: 9140677Abstract: In exemplary implementations of this invention, an electronic olfactor determines whether a scent being tested matches the scent of a positive control. The electronic olfactor can perform this scent matching even in a changing olfactory environment, and even if the positive control scent is a combination of hundreds or thousands of different odorants. No prior training is needed, and no attempt is made to identify a single odorant that is unambiguously responsible for a scent. Instead, a computer compares the total scent pattern of a positive control sample with the total scent pattern of a test sample, across a sweep of many permutations of electrical inputs to scent sensors, to try to find any condition under which the total scent patterns do not match. If such a condition cannot be found, then the computer declares a match between the test and target scents.Type: GrantFiled: August 13, 2013Date of Patent: September 22, 2015Assignee: Massachusetts Institute of TechnologyInventors: Andreas Mershin, Asmamaw Wassie, Yael Maguire, David Kong, Shuguang Zhang, Patrick Moran, Karolina Corin
-
Publication number: 20140099729Abstract: In exemplary implementations of this invention, an electronic olfactor determines whether a scent being tested matches the scent of a positive control. The electronic olfactor can perform this scent matching even in a changing olfactory environment, and even if the positive control scent is a combination of hundreds or thousands of different odorants. No prior training is needed, and no attempt is made to identify a single odorant that is unambiguously responsible for a scent. Instead, a computer compares the total scent pattern of a positive control sample with the total scent pattern of a test sample, across a sweep of many permutations of electrical inputs to scent sensors, to try to find any condition under which the total scent patterns do not match. If such a condition cannot be found, then the computer declares a match between the test and target scents.Type: ApplicationFiled: August 13, 2013Publication date: April 10, 2014Inventors: Andreas Mershin, Asmamaw Wassie, Yael Maguire, David Kong, Shuguang Zhang, Patrick Moran, Karolina Corin