Abstract: Aquaculture systems and methods are provided, as well as immunogenic compositions and methods of immunologically protecting bivalves against specified pathogens. Methods include inactivating specified pathogens using UV (ultraviolet) radiation, exposing invertebrates grown or to be grown in aquaculture to the specified UV-inactivated pathogens to enhance an immune reaction of the exposed invertebrates toward the specified pathogens, and growing the exposed invertebrates in aquaculture. For example, the methods were demonstrated to increase the immune response of oysters to ostreid herpesvirus 1 (OsHV-1) following their prior exposure to UV-inactivated OsHV-1.

Abstract: The invention relates to processes for preparing aqueous nitric oxide solutions, by means of introducing a nitric oxide gas stream into an acidic, halide-containing aqueous solution, or by means of nitrite reduction in an acidic environment in the presence of halide ion. The resultant solutions are stable and can be used for treating surfaces prone to the formation of biofilm, or having biofilm thereon.