Abstract: The present invention comprises an improved method of manufacturing platelet concentrate comprising the steps of: performing a first centrifugation of anticoagulated blood, pooling a first platelet rich sample, performing a second centrifugation on the first platelet rich sample whereby the volume of the first platelet rich sample placed in each subsequent centrifuge container is greater than the extracted volume from each previously utilized container, pooling a second platelet rich sample, performing a third centrifugation on the second platelet rich sample, whereby the volume of the second platelet rich sample placed in each subsequent centrifuge container is greater than the previous extracted volume from each previously utilized container, decanting a portion of supernatant overlying a third platelet rich sample, and suspending the third platelet rich sample such as to form a highly concentrated platelet rich formulation at concentrations greater than 8 times baseline and up to 60 times baseline.