Abstract: An object of the present invention is to provide an immunochromatographic kit and a method, which are capable of detecting Mycobacterium tuberculosis with high-sensitivity and specificity. According to the present invention, an immunochromatographic kit for detecting Mycobacterium tuberculosis is provided, the kit including: a label substance modified with a first antibody against lipoarabinomannan; a porous carrier having a reaction site holding a second antibody against lipoarabinomannan; a compound containing silver; and a reducing agent reducing silver ions, in which at least one of the first antibody or the second antibody is a monoclonal antibody.

Abstract: An object of the present invention is to provide a chromatographic kit and a chromatographic method which have high-sensitivity and in which occurrence of false positives is inhibited. According to the present invention, an immunochromatographic kit is provided, the immunochromatographic kit including: a label substance modified with a first antibody having binding properties with respect to a test substance; a porous carrier having a reaction site holding a second antibody having binding properties with respect to the test substance; an amplification reagent; and a third antibody having an immune host which is the same as that of at least one antibody of the first antibody or the second antibody and not recognizing the test substance.

Abstract: An immunochromatography includes steps of mixing a specimen capable of containing an antigen and a modified particle, which is a particle modified with a substance having a specific affinity to the antigen, to obtain a mixture containing particle composite bodies; sedimenting the particle composite bodies in the mixture using a centrifuge; dissociating the sedimented particle composite bodies into the particles and the antigen by mixing the sedimented particle composite bodies with a dissociation solution, recovering an antigen-concentrated solution by sedimenting the dissociated particles using a centrifuge; neutralizing the antigen-concentrated solution using a neutralization solution; spreading particle composite bodies for labeling on an insoluble carrier having a reaction site, in a state where the particle composite bodies for labeling, which are composite bodies of the antigen in the neutralized antigen-concentrated solution and a modified particle for labeling, are formed; and capturing the particle c

Abstract: An immunochromatography including steps of mixing an antigen-containable specimen and modified magnetic particles, which are magnetic particles modified with a substance having a specific affinity to the antigen; collecting the magnetic particles using magnetism; dissociating the modified magnetic particles to obtain an antigen-concentrated solution by mixing the collected magnetic particles with a dissociation solution, an amount of which is smaller than the antigen-containable specimen; obtaining a neutralized antigen-concentrated solution; spreading gold particle composite bodies on an insoluble carrier having a reaction site at which a second binding substance has been immobilized, in a state where the gold particle composite bodies which are composite bodies of an antigen in the neutralized antigen-concentrated solution and modified gold particles which are gold particles modified with a first binding substance are formed; capturing the gold particle composite bodies at the reaction site; and silver-ampl

Abstract: An immunochromatography including steps of mixing an antigen-containable specimen and modified magnetic particles, which are magnetic particles modified with a monoclonal antibody X; collecting the magnetic particles from the mixture using magnetism; dissociating the modified magnetic particles to obtain an antigen-containing solution; neutralizing the antigen-containing solution to obtaina neutralized antigen-containing solution; spreading gold particle composite bodies on an insoluble carrier having a reaction site at which a monoclonal antibody Z has been immobilized, wherein the gold particle composite body is a composite body of the antigen and a modified gold particle which is a gold particle modified with a monoclonal antibody Y; capturing the gold particle composite bodies at the reaction site; and silver-amplifying the gold particle composite body, in which the antibody X and the antibody Y are different from each other, and the antibody X and the antibody Z are different from each other.

Abstract: The immunological test method includes a concentration step of concentrating an antigen-containable solution by mixing the antigen-containable solution with a superabsorbent polymer to obtain an antigen-concentrated solution, and a detection step of detecting an antigen in the antigen-concentrated solution using an antigen-antibody reaction, in which a swelling ratio of the superabsorbent polymer is more than 0.2 g/g and less than 800 g/g, and an antibody that is used in the antigen-antibody reaction is a monoclonal antibody.

Abstract: A nozzle includes a mounting opening that is to be connected to an opening portion of a container, an outlet that jets liquid stored in the container, a side face that connects the mounting opening to the outlet, and a liquid storage portion that stores a part of the liquid stored in the container in the nozzle without jetting a part of the liquid from the outlet in a case where the liquid is to be jetted from the dispensing container, that is, in a case where the outlet faces downward in the direction of gravity.

Abstract: An object of the present invention is to provide immunochromatography exhibiting excellent magnetic detection sensitivity.

Abstract: An object of the present invention is to provide an immunochromatographic kit and a method, which are capable of detecting Mycobacterium tuberculosis with high-sensitivity and specificity. According to the present invention, an immunochromatographic kit for detecting Mycobacterium tuberculosis is provided, the kit including: a label substance modified with a first antibody against lipoarabinomannan; a porous carrier having a reaction site holding a second antibody against lipoarabinomannan; a compound containing silver; and a reducing agent reducing silver ions, in which at least one of the first antibody or the second antibody is a monoclonal antibody.

Abstract: A housing case that includes an inspection strip, a first pot in which a first amplification liquid is sealed, and a second pot in which a second amplification liquid is sealed, includes a lower case, an upper case, and an intermediate member disposed therebetween, in which the upper case includes, on a part facing the first pot, a first protrusive deforming portion that deforms towards a side of the first pot when applied with pressing force from outside, whereby a tearing portion of the intermediate member tears a sheet member of the first pot, and includes, at a part facing the second pot, a second protrusive deforming portion that deforms towards a side of the second pot when applied with pressing force from outside, whereby a sheet member of the second pot is torn.

Abstract: The immunochromatographic kit includes an inspection strip which includes an insoluble carrier spreading the specimen liquid, a label-holding pad including a label substance modified with a first substance bondable to a test substance, a liquid-sending pad sending a first amplification liquid to the insoluble carrier, and an absorption pad disposed in contact with the other end of the insoluble carrier and sequentially has an inspection region including a second substance being bonded to the test substance, a confirmation region including a substance bondable to the first substance, and an amplification index region including a substance being reacted with the first amplification liquid between the label-holding pad and the absorption pad of the insoluble carrier, a first pot being disposed below the liquid-sending pad and enclosing the first amplification liquid, and a second pot being disposed above the absorption pad and enclosing a second amplification liquid in a housing case.

Abstract: A nozzle includes a mounting opening that is to be connected to an opening portion of a container, an outlet that jets liquid stored in the container, a side face that connects the mounting opening to the outlet, and a liquid storage portion that stores a part of the liquid stored in the container in the nozzle without jetting a part of the liquid from the outlet in a case where the liquid is to be jetted from the dispensing container, that is, in a case where the outlet faces downward in the direction of gravity.

Abstract: A housing case that includes an inspection strip, a first pot in which a first amplification liquid is sealed, and a second pot in which a second amplification liquid is sealed, includes a lower case, an upper case, and an intermediate member disposed therebetween, in which the upper case includes, on a part facing the first pot, a first protrusive deforming portion that deforms towards a side of the first pot when applied with pressing force from outside, whereby a tearing portion of the intermediate member tears a sheet member of the first pot, and includes, at a part facing the second pot, a second protrusive deforming portion that deforms towards a side of the second pot when applied with pressing force from outside, whereby a sheet member of the second pot is torn.

Abstract: The immunochromatographic kit includes an inspection strip which includes an insoluble carrier spreading the specimen liquid, a label-holding pad including a label substance modified with a first substance bondable to a test substance, a liquid-sending pad sending a first amplification liquid to the insoluble carrier, and an absorption pad disposed in contact with the other end of the insoluble carrier and sequentially has an inspection region including a second substance being bonded to the test substance, a confirmation region including a substance bondable to the first substance, and an amplification index region including a substance being reacted with the first amplification liquid between the label-holding pad and the absorption pad of the insoluble carrier, a first pot being disposed below the liquid-sending pad and enclosing the first amplification liquid, and a second pot being disposed above the absorption pad and enclosing a second amplification liquid in a housing case.

Abstract: The chromatography method of the present invention includes (a) developing a complex of a test substance and a labeling substance modified with a first binding substance bindable to the test substance, onto an insoluble carrier, (b) causing the complex to be trapped in a reaction site on the insoluble carrier which contains a second binding substance bindable to the test substance or a substance exhibiting binding properties to the first binding substance, (c) washing the insoluble carrier with a washing solution containing at least one of the potassium iodide, urea, and guanidine after the step (b), (d) washing off the washing solution of the step (c) remaining in the insoluble carrier from the insoluble carrier, and (e) amplifying the labeling substance of the complex trapped in the reaction site.

Abstract: The chromatography method includes a step of forming a composite with a test substance and a labeling substance containing a metal modified by a first binding substance of the test substance and then developing the composite on an insoluble carrier; a step of capturing the test substance and the labeling substance in a detection site on the insoluble carrier including a second binding substance of the test substance or a substance having a binding property to the first binding substance of the test substance; and a step of amplifying the captured labeling substance using a first amplification reagent and a second amplification reagent to detect the test substance.

Abstract: The chromatography method of the present invention includes (a) developing a complex of a test substance and a labeling substance modified with a first binding substance bindable to the test substance, onto an insoluble carrier, (b) causing the complex to be trapped in a reaction site on the insoluble carrier which contains a second binding substance bindable to the test substance or a substance exhibiting binding properties to the first binding substance, (c) washing the insoluble carrier with a washing solution containing at least one of the potassium iodide, urea, and guanidine after the step (b), (d) washing off the washing solution of the step (c) remaining in the insoluble carrier from the insoluble carrier, and (e) amplifying the labeling substance of the complex trapped in the reaction site.

Abstract: A chromatography method including a step of developing a complex of a specimen and a labeled substance modified by a first bondable substance which can be bonded to the specimen on an insoluble carrier, a step of capturing the complex of the specimen and the labeled substance at a reactive site on the insoluble carrier, a step of washing the reactive site on the insoluble carrier using a washing liquid, a step of detecting the specimen which is captured at the reactive site after a signal of the labeled substance is amplified by an amplification liquid, in which the reactive site on the insoluble carrier includes (i) a second bondable substance which can be bonded to the specimen or a substance having affinity to the first bondable substance which can be bonded to the specimen, and (ii) polyethylene glycol having the molecular weight from 1,500 to 10,000.

Abstract: A chromatographic kit is provided including a labeling substance holding area having a labeling substance modified with a first binding substance of a test substance, and a labeling substance capturing area having a second binding substance of the test substance or a binding substance of the first binding substance in this order from upstream to downstream of a development direction of a test sample including the test substance, and further including an area having a color developing reagent in order to detect a first amplification reagent of two types of amplification reagents used to amplify the signal of the labeling substance when detecting the labeling substance.

Abstract: The chromatography method includes a step of forming a composite with a test substance and a labeling substance containing a metal modified by a first binding substance of the test substance and then developing the composite on an insoluble carrier; a step of capturing the test substance and the labeling substance in a detection site on the insoluble carrier including a second binding substance of the test substance or a substance having a binding property to the first binding substance of the test substance; and a step of amplifying the captured labeling substance using a first amplification reagent and a second amplification reagent to detect the test substance.