Abstract: A probe comprising a reporter region for detecting a first single nucleotide polymorphism, an anchor region for detecting a second single nucleotide polymorphism, and a linker region is disclosed. The reporter region comprises an oligonucleotide consisting of a sequence perfectly matching with the first target sequence, and a fluorescent dye that quenches when hybridized to the first target sequence where the first single nucleotide polymorphism is present. The anchor region comprises an oligonucleotide consisting of a sequence perfectly matching with a second target sequence where the second single nucleotide polymorphism is present. A length of the oligonucleotide of the reporter region is shorter than a length of the oligonucleotide of the anchor region.

Abstract: The present invention provides a sensitive and simple detection method, and a kit therefor, which detects residual undifferentiated human pluripotent stem cells in an intermediate product and/or a final product of a regenerative medical products, using an isothermal nucleic acid amplification method, and specifically, a LAMP method. A method for detecting presence or absence of undifferentiated cells in a non-undifferentiated cell population, wherein RNA derived from an undifferentiation marker gene exhibiting a significant difference in expression level between the undifferentiated cells and the non-undifferentiated cells in a sample containing a nucleic acid derived from the cell population of interest is detected by the isothermal nucleic acid amplification method.

Abstract: A probe comprising a reporter region for detecting a first single nucleotide polymorphism, an anchor region for detecting a second single nucleotide polymorphism, and a linker region is disclosed. The reporter region comprises an oligonucleotide consisting of a sequence perfectly matching with the first target sequence, and a fluorescent dye that quenches when hybridized to the first target sequence where the first single nucleotide polymorphism is present. The anchor region comprises an oligonucleotide consisting of a sequence perfectly matching with a second target sequence where the second single nucleotide polymorphism is present. A length of the oligonucleotide of the reporter region is shorter than a length of the oligonucleotide of the anchor region.