Patents by Inventor Atsumu Hirabayashi
Atsumu Hirabayashi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8674299Abstract: A mass spectrometric device of the present invention includes a quadrupole filter (12) located upstream of a quadrupole ion trap (13) and configured to transmit ions in a predetermined filter range, and determines the filter range of the quadrupole filter (12) such that accumulation time for the ions in the quadrupole ion trap (13) is maximized. The accumulation time for the ions is determined based on mass spectrometry data information. With this configuration, the present invention produces advantageous effects of improving analysis throughput and an S/N ratio in an analysis of a minor sample component mixed in various accompanying components by using the mass spectrometric device using the quadrupole ion trap.Type: GrantFiled: February 15, 2010Date of Patent: March 18, 2014Assignee: Hitachi High-Technologies CorporationInventors: Atsumu Hirabayashi, Hiroyuki Satake
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Publication number: 20120305762Abstract: Disclosed is a method whereby predetermined ions are isolated and ions to be left in an ion trap are left at the time of performing mass spectrometry using the ion trap. In order to have high ion isolation accuracy and to shorten a time necessary for ion isolation, a first time wherein ions having a lower mass than the ions to be left are isolated is set shorter than a second time wherein ions having a higher mass than the ions to be left are isolated.Type: ApplicationFiled: December 13, 2010Publication date: December 6, 2012Inventors: Akihito Kaneko, Atsumu Hirabayashi
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Publication number: 20110315868Abstract: A mass spectrometric device of the present invention includes a quadrupole filter (12) located upstream of a quadrupole ion trap (13) and configured to transmit ions in a predetermined filter range, and determines the filter range of the quadrupole filter (12) such that accumulation time for the ions in the quadrupole ion trap (13) is maximized. The accumulation time for the ions is determined based on mass spectrometry data information. With this configuration, the present invention produces advantageous effects of improving analysis throughput and an S/N ratio in an analysis of a minor sample component mixed in various accompanying components by using the mass spectrometric device using the quadrupole ion trap.Type: ApplicationFiled: February 15, 2010Publication date: December 29, 2011Inventors: Atsumu Hirabayashi, Hiroyuki Satake
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Publication number: 20110101215Abstract: In quantitation without using the isotope labeling technique, there is no means to detect the presence/absence and the time region of the occurrence of quantitative analysis-inhibitory factors in data for the analysis, and the reliability of the data for the analysis cannot be evaluated. Also, the error of the data due to the occurrence of the quantitative analysis-inhibitory factors cannot be evaluated. In order to solve the problems, first, an internal standard to be detected simultaneously with a component for the analysis is mixed in a mobile phase or an eluate of a liquid chromatograph; under the condition where no quantitative analysis-inhibitory factors occur, a blank sample is analyzed to acquire a mass chromatogram of ions originated from the internal standard; and the result is stored in a data storage unit.Type: ApplicationFiled: March 27, 2009Publication date: May 5, 2011Inventors: Atsumu Hirabayashi, Masako Ishimaru, Kiyomi Yoshinari, Naomi Manri
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Patent number: 7932486Abstract: During the structural analysis of a protein or peptide by tandem mass spectroscopy, a peptide ion derived from a protein that has already been measured and that is expressed in great quantities is avoided as a tandem mass spectroscopy target. A peptide derived from a minute amount of protein, which has heretofore been difficult to analyze, can be automatically determined as a tandem mass spectroscopy target within the real time of measurement. Data concerning a protein that has already been measured and a peptide derived from the protein is automatically stored in an internal database. The stored data is collated with measured data with high accuracy to determine an isotope peak. In this way, the process of selecting a peptide peak that has not been measured as the target for the next tandem analysis can be performed within the real time of measurement and a redundant measurement of peptides derived from the same protein can be avoided.Type: GrantFiled: December 8, 2008Date of Patent: April 26, 2011Assignee: Hitachi High-Technologies CorporationInventors: Akihiro Sano, Atsumu Hirabayashi, Yasushi Terui, Kinya Kobayashi, Kiyomi Yoshinari, Kenko Uchida, Toshiyuki Yokosuka
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Patent number: 7829845Abstract: To analyze glycans and peptide sequences without liberating glycans from glycosylated peptides, a glycan structure is analyzed through negative-ion CID, in which sialic acid and fucose are resistant to elimination, and a peptide sequence is analyzed through positive-ion ECD.Type: GrantFiled: July 23, 2007Date of Patent: November 9, 2010Assignee: Hitachi High-Technologies CorporationInventors: Kisaburo Deguchi, Atsumu Hirabayashi, Hiroki Ito, Takashi Baba
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Patent number: 7645983Abstract: An ion source includes a body having a gas passage and an orifice. A capillary is inserted into the gas passage so that a tip portion of the capillary extends into the orifice. A gas supplier supplies a gas into the gas passage to form a gas flow through the gas passage along the capillary and through the orifice past a tip of the capillary so that the gas flow sprays a sample solution flowing through the capillary from the tip of the capillary. A flow controller regulates a pressure of the gas in the gas passage to adjust a characteristic value F/S to a predetermined value, where F is a flow rate of the gas flow at standard conditions (20° C., 1 atmosphere), and S is a difference between a cross section of the orifice and a cross section of the tip portion of the capillary in the orifice.Type: GrantFiled: May 7, 2002Date of Patent: January 12, 2010Assignee: Hitachi, Ltd.Inventors: Atsumu Hirabayashi, Minoru Sakairi, Yasuaki Takada, Hideaki Koizumi, Kaoru Umemura
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Patent number: 7595484Abstract: The present invention can provide a mass spectrometric system judging whether a measurement target is a substance required by an operator within an actual measurement time, when a substance (particularly such as protein or sugar chains) is analyzed. In the mass spectrometric system using a tandem mass spectrometer, a particular substance obtained by separating a sample is ionized, and mass analysis of the ionized substance is performed to obtain a spectrum. This spectrum is compared with a particular spectrum stored in advance, to thereby determine whether both the spectra match with each other. When a match is determined, a particular ion is further ionized within a particular time for detailed analysis. The invention also provides a mass spectrometric method, a diagnosis system and an inspection system each using the mass spectrometric system, and a program for operating a computer to control those systems with desired functions.Type: GrantFiled: December 23, 2005Date of Patent: September 29, 2009Assignee: Hitachi High-Technologies Corp.Inventors: Toshiyuki Yokosuka, Kinya Kobayashi, Kiyomi Yoshinari, Atsushi Otake, Atsumu Hirabayashi, Yasushi Terui
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Publication number: 20090189063Abstract: During the structural analysis of a protein or peptide by tandem mass spectroscopy, a peptide ion derived from a protein that has already been measured and that is expressed in great quantities is avoided as a tandem mass spectroscopy target. A peptide derived from a minute amount of protein, which has heretofore been difficult to analyze, can be automatically determined as a tandem mass spectroscopy target within the real time of measurement. Data concerning a protein that has already been measured and a peptide derived from the protein is automatically stored in an internal database. The stored data is collated with measured data with high accuracy to determine an isotope peak. In this way, the process of selecting a peptide peak that has not been measured as the target for the next tandem analysis can be performed within the real time of measurement and a redundant measurement of peptides derived from the same protein can be avoided.Type: ApplicationFiled: December 8, 2008Publication date: July 30, 2009Inventors: Akihiro Sano, Atsumu Hirabayashi, Yasushi Terui, Kinya Kobayashi, Kiyomi Yoshinari, Kenko Uchida, Toshiyuki Yokosuka
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Patent number: 7544930Abstract: The present invention provides a tandem type mass analysis system capable of carrying out the differential analysis with high efficiency by the tandem type mass analysis. A predetermined number of m/z regions are set up for carrying out the mass analysis with the all ions included therein being dissociated collectively for each m/z region so as to obtain measurement MS2 data. By comparing the measurement MS2 data with reference MS2 data stored in a reference data base, a difference thereof is detected. For the m/z region with a differential component detected, the mass analysis is carried out collectively without dissociation for the all ions included therein so as to obtain measurement MS1 data. By comparing the measurement MS1 data with the reference MS1 data, a difference thereof is detected. From the difference thereof, a parent ion considered to be the differential component factor is presumed for carrying out the mass analysis with the same being dissociated.Type: GrantFiled: January 18, 2007Date of Patent: June 9, 2009Assignee: Hitachi High-Technologies CorporationInventors: Kiyomi Yoshinari, Yasushi Terui, Toshiyuki Yokosuka, Kinya Kobayashi, Atsumu Hirabayashi
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Patent number: 7538321Abstract: An object of the present invention is to provide a mass spectrometry system capable of improving an efficiency of obtaining information on a structure of substances, shortening a time taken for a measurement and substance identification, and improving identification accuracy. The system comprises: a process of mass analyzing an ionized analyte; a first fragmentation process where a first ion is selected from the ions observed in a mass spectrometry to fragment it; a process of mass analyzing a plurality of the ions generated in the first fragmentation process; a process of determining fragment ion combination capable of reconstructing the first ion using a result of the mass spectrometry; a second fragmentation process where the fragment ions contained in the fragment ion combination are fragmented; and a process of mass analyzing the fragment ions generated in the second fragmentation.Type: GrantFiled: May 11, 2006Date of Patent: May 26, 2009Assignee: Hitachi High-Technologies CorporationInventors: Masako Ishimaru, Atsumu Hirabayashi, Kinya Kobayashi, Kisaburo Deguchi
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Patent number: 7473892Abstract: During the structural analysis of a protein or peptide by tandem mass spectroscopy, a peptide ion derived from a protein that has already been measured and that is expressed in great quantities is avoided as a tandem mass spectroscopy target. A peptide derived from a minute amount of protein, which has heretofore been difficult to analyze, can be automatically determined as a tandem mass spectroscopy target within the real time of measurement. Data concerning a protein that has already been measured and a peptide derived from the protein is automatically stored in an internal database. The stored data is collated with measured data with high accuracy to determine an isotope peak. In this way, the process of selecting a peptide peak that has not been measured as the target for the next tandem analysis can be performed within the real time of measurement and a redundant measurement of peptides derived from the same protein can be avoided.Type: GrantFiled: May 20, 2004Date of Patent: January 6, 2009Assignee: Hitachi High-Technologies CorporationInventors: Akihiro Sano, Atsumu Hirabayashi, Yasushi Terui, Kinya Kobayashi, Kiyomi Yoshinari, Kenko Uchida, Toshiyuki Yokosuka
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Publication number: 20080237459Abstract: An ion source includes a body having a gas passage and an orifice. A capillary is inserted into the gas passage so that a tip portion of the capillary extends into the orifice. A gas supplier supplies a gas into the gas passage to form a gas flow through the gas passage along the capillary and through the orifice past a tip of the capillary so that the gas flow sprays a sample solution flowing through the capillary from the tip of the capillary. A flow controller regulates a pressure of the gas in the gas passage to adjust a characteristic value F/S to a predetermined value, where F is a flow rate of the gas flow at standard conditions (20.degree. C., 1 atmosphere), and S is a difference between a cross section of the orifice and a cross section of the tip portion of the capillary in the orifice.Type: ApplicationFiled: April 18, 2008Publication date: October 2, 2008Inventors: Atsumu Hirabayashi, Minoru Sakairi, Yasuaki Takada, Hideaki Koizumi, Kaoru Umemura
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Publication number: 20080048110Abstract: To analyze glycans and peptide sequences without liberating glycans from glycosylated peptides, a glycan structure is analyzed through negative-ion CID, in which sialic acid and fucose are resistant to elimination, and a peptide sequence is analyzed through positive-ion ECD.Type: ApplicationFiled: July 23, 2007Publication date: February 28, 2008Inventors: Kisaburo DEGUCHI, Atsumu HIRABAYASHI, Hiroki ITO, Takashi BABA
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Publication number: 20070221836Abstract: An object of the present invention is to evaluate quantitatively a peptide derived from a protein, whose analysis has been difficult so far, by analyzing a peptide ion derived from a protein already measured but having a different total ion amount as the tandem mass analysis target at the time of quantitatively evaluating a fluctuating component between different kinds of specimens by the tandem mass analysis of a protein or a peptide. In the present invention, in order to achieve the above-mentioned object, data of a derived peptide obtained by a first time measurement are stored automatically in an internal database and collated with second time measurement data highly accurately. The processing for selecting the peak of the already measured peptide with the relative amount fluctuation as the next tandem analysis target is implemented within the real time of the measurement for avoiding the analysis of a peptide without the relative amount fluctuation.Type: ApplicationFiled: January 26, 2007Publication date: September 27, 2007Inventors: Kinya Kobayashi, Kiyomi Yoshinari, Toshiyuki Yokosuka, Atsumu Hirabayashi
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Publication number: 20070187588Abstract: The present invention provides a tandem type mass analysis system capable of carrying out the differential analysis with high efficiency by the tandem type mass analysis. A predetermined number of m/z regions are set up for carrying out the mass analysis with the all ions included therein being dissociated collectively for each m/z region so as to obtain measurement MS2 data. By comparing the measurement MS2 data with reference MS2 data stored in a reference data base, a difference thereof is detected. For the m/z region with a differential component detected, the mass analysis is carried out collectively without dissociation for the all ions included therein so as to obtain measurement MS1 data. By comparing the measurement MS1 data with the reference MS1 data, a difference thereof is detected. From the difference thereof, a parent ion considered to be the differential component factor is presumed for carrying out the mass analysis with the same being dissociated.Type: ApplicationFiled: January 18, 2007Publication date: August 16, 2007Inventors: Kiyomi Yoshinari, Yasushi Terui, Toshiyuki Yokosuka, Kinya Kobayashi, Atsumu Hirabayashi
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Patent number: 7180056Abstract: In a method of measuring by a tandem mass spectrometer a sample labeled with an isotope, measuring throughput is improved. In a technique in which tandem mass spectrometer is used to analyze a sample labeled with an isotope, spectra obtained by a first-stage measurement (MS1) are analyzed during a measuring session in a realtime fashion to determine ions to be used in second-stage and subsequent dissociation• spectral measurement (MS2).Type: GrantFiled: June 3, 2005Date of Patent: February 20, 2007Assignee: Hitachi, Ltd.Inventors: Atsushi Ohtake, Kinya Kobayashi, Kiyomi Yoshinari, Toshiyuki Yokosuka, Atsumu Hirabayashi
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Patent number: 7141788Abstract: A mass spectrometric apparatus of high sensitivity, including a spray ionization interface suitable for the ionization of a low flow-rate liquid that prevents charged particles from being introduced into a vacuum device; wherein the ion source comprises a capillary having a first end having an inner diameter that gradually reduces in size in the direction of gas flow and wherein a liquid sample is introduced into an opposite second end of the capillary; a gas guide tube which guides gas flow along an outer periphery of the first end the capillary and which sprays the liquid sample from the first end of the capillary; and a gas introducing section for introducing the gas into the gas guide tube. A first end of the gas guide tube has a reduced inside diameter and receives the first end of the capillary in a holding member. Gaseous ions produced are introduced into a vacuum section through an ion intake port and are subjected to mass separation by a mass spectrometer.Type: GrantFiled: October 29, 2004Date of Patent: November 28, 2006Assignee: Hitachi, Ltd.Inventors: Atsumu Hirabayashi, Yuichiro Hashimoto
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Publication number: 20060255263Abstract: An object of the present invention is to provide a mass spectrometry system capable of improving an efficiency of obtaining information on a structure of substances, shortening a time taken for a measurement and substance identification, and improving identification accuracy. The system comprises: a process of mass analyzing an ionized analyte; a first fragmentation process where a first ion is selected from the ions observed in a mass spectrometry to fragment it; a process of mass analyzing a plurality of the ions generated in the first fragmentation process; a process of determining fragment ion combination capable of reconstructing the first ion using a result of the mass spectrometry; a second fragmentation process where the fragment ions contained in the fragment ion combination are fragmented; and a process of mass analyzing the fragment ions generated in the second fragmentation.Type: ApplicationFiled: May 11, 2006Publication date: November 16, 2006Inventors: Masako Ishimaru, Atsumu Hirabayashi, Kinya Kobayashi, Kisaburo Deguchi
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Patent number: 7126113Abstract: According to the existing mass spectrometric system, whether or not the informations are sufficient for analyzing substances (particularly proteins, sugars, etc.) cannot be judged in the process of measurement. Further, it is difficult to find out isomers having just the same mass number or compounds very close in mass only from the MS data. According to this invention, whether or not the retention time in the LC (or GC) of peptide formed at the time of enzymatic decomposition of protein coincides with the predicted retention time assumed from the amino acid sequence predicted from MS2 mass spectrometry data is judged within the actual time period of measurement, and thereby the quality of MS2 mass spectrometry data (quantity of information) is judged.Type: GrantFiled: January 25, 2005Date of Patent: October 24, 2006Assignee: Hitachi High-Technologies CorporationInventors: Toshiyuki Yokosuka, Atsumu Hirabayashi, Yasushi Terui, Kinya Kobayashi, Kiyomi Yoshinari