Patents by Inventor Atsushi Miyawaki

Atsushi Miyawaki has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20240111366
    Abstract: An information processing apparatus comprises a processor configured to execute a program so as to: acquire viewpoint information, motion information and object information; process an object image wherein: the object image is an image representing a virtual substance and is updated based on the viewpoint information and the motion information; calculate a position when superimposing a background image and the object image, wherein: the background image is an image according to the field of view of the user; generate visual information superimposing the background image and the object image based on the position calculated, and output the generated visual information to a display device; and generate tactile information for an ultrasound generator irradiating the user with ultrasound corresponding to the object based on the position calculated, the motion information and the object information, and output the generated tactile information to the ultrasound generator.
    Type: Application
    Filed: December 8, 2022
    Publication date: April 4, 2024
    Inventors: Mamoru MIYAWAKI, Hiroyuki SHINODA, Takaaki KAMIGAKI, Mitsuru ITO, Tao MORISAKI, Shun SUZUKI, Atsushi MATSUBAYASHI, Ryoya ONISHI, Yasutoshi MAKINO, Seki INOUE
  • Publication number: 20220380419
    Abstract: A fluorescent protein of the present invention has an amino acid sequence of a green fluorescent protein (GFP) derived from a crystal jelly or of a mutant fluorescent protein of the green fluorescent protein, the amino acid sequence having an amino acid residue (alanine residue) substituted with a phenylalanine residue, the amino acid residue corresponding to position 206 with the amino acid sequence of the GFP used as a reference sequence.
    Type: Application
    Filed: October 2, 2020
    Publication date: December 1, 2022
    Inventors: Yusuke NIINO, Atsushi MIYAWAKI
  • Patent number: 11203621
    Abstract: The present invention provides a tool which exhibits excellent properties in the quantification of autophagy activity. A unimolecular FRET probe of the present invention includes an acceptor consisting of a fluorescent protein to be enzymatically degraded inside a lysosome or a vacuole; and a donor having an amino acid sequence having a sequence identity of 95% or more with respect to an amino acid sequence represented by SEQ ID NO: 1.
    Type: Grant
    Filed: June 17, 2016
    Date of Patent: December 21, 2021
    Assignee: RIKEN
    Inventors: Atsushi Miyawaki, Hiroyuki Katayama
  • Publication number: 20210131926
    Abstract: A clearing reagent according to this invention for making a biological material transparent is a solution containing, as an active component, at least one compound selected from the group consisting of urea and urea derivatives, in order to provide a novel clearing reagent for making a biological material transparent.
    Type: Application
    Filed: December 21, 2020
    Publication date: May 6, 2021
    Inventors: Atsushi Miyawaki, Hiroshi Hama, Hiroshi Kurokawa, Hiroyuki Kawano, Asako Sakaue
  • Publication number: 20210131925
    Abstract: A method for making a biological material transparent according to the present invention includes: a first permeation step of causing a first solution to permeate into a biological material, the first solution containing at least one compound selected from the group consisting of urea and urea derivatives at a predetermined concentration; and then a second permeation step of causing a second solution to permeate into the biological material, the second solution containing at least one compound selected from the group consisting of urea and urea derivatives at a concentration higher than the concentration of the at least one compound contained in the first solution.
    Type: Application
    Filed: December 21, 2020
    Publication date: May 6, 2021
    Inventors: Atsushi Miyawaki, Hiroshi Hama, Hiroshi Kurokawa
  • Patent number: 10815462
    Abstract: In order to provide modified luciferase whose substrate specificity to at least one luminescent substrate (e.g., AkaLumine) other than D-luciferin has been improved as compared with to D-luciferin, modified luciferase according to an aspect of the present invention has a mutation at an amino acid corresponding to a 347th amino acid in an amino acid sequence represented by SEQ ID NO: 1.
    Type: Grant
    Filed: August 25, 2017
    Date of Patent: October 27, 2020
    Assignee: RIKEN
    Inventors: Atsushi Miyawaki, Satoshi Iwano
  • Patent number: 10513676
    Abstract: Provided is a measurement method whereby the amount of unbound bilirubin (UB) can be exactly reflected whether a specimen contains a large amount of conjugated bilirubin or not. The measurement method for UB according to the present invention comprises decomposition step (i), decomposition stopping step (ii), contact step (iii) and detection step (iv). In decomposition step (i), a blood sample containing unconjugated bilirubin (iD-Bil) and conjugated bilirubin (D-Bil) is subjected to an oxidative decomposition reaction of UB in iD-Bil and D-Bil. In decomposition stopping step (ii), the oxidative decomposition reaction is stopped to give a decomposition product of the sample. In contact step (iii), the decomposition product of the sample is contacted with UnaG that is capable of specifically binding to iD-Bil. Separately, an unreacted sample, which is the blood sample not subjected to decomposition step (i), is contacted with UnaG too.
    Type: Grant
    Filed: March 30, 2016
    Date of Patent: December 24, 2019
    Assignee: National University Corporation Kobe University
    Inventors: Sota Iwatani, Ichiro Morioka, Hajime Nakamura, Atsushi Miyawaki, Akiko Kumagai
  • Patent number: 10444124
    Abstract: A clearing reagent according to the present invention for making a biological material transparent is a solution containing: at a concentration of 1M or more and not more than 8.5M, at least one compound selected from the group consisting of urea and urea derivatives; and glycerol at a concentration of 25 (w/v) % or more and not more than 35 (w/v) %.
    Type: Grant
    Filed: May 18, 2012
    Date of Patent: October 15, 2019
    Assignee: RIKEN
    Inventors: Atsushi Miyawaki, Hiroshi Hama, Asako Sakaue
  • Publication number: 20190177704
    Abstract: In order to provide modified luciferase whose substrate specificity to at least one luminescent substrate (e.g., AkaLumine) other than D-luciferin has been improved as compared with to D-luciferin, modified luciferase according to an aspect of the present invention has a mutation at an amino acid corresponding to a 347th amino acid in an amino acid sequence represented by SEQ ID NO: 1.
    Type: Application
    Filed: August 25, 2017
    Publication date: June 13, 2019
    Inventors: Atsushi MIYAWAKI, Satoshi IWANO
  • Patent number: 10254276
    Abstract: An antibody composition, which is an aspect of the present invention, contains at least one compound selected from the group consisting of urea and urea derivatives, the compound being contained in the antibody composition at a concentration of not less than 0.1 M and less than 1 M, the antibody composition being a solution.
    Type: Grant
    Filed: July 10, 2013
    Date of Patent: April 9, 2019
    Assignee: RIKEN
    Inventors: Atsushi Miyawaki, Hiroshi Hama
  • Publication number: 20180251500
    Abstract: The present invention provides a tool which exhibits excellent properties in the quantification of autophagy activity. A unimolecular FRET probe of the present invention includes an acceptor consisting of a fluorescent protein to be enzymatically degraded inside a lysosome or a vacuole; and a donor having an amino acid sequence having a sequence identity of 95% or more with respect to an amino acid sequence represented by SEQ ID NO: 1.
    Type: Application
    Filed: June 17, 2016
    Publication date: September 6, 2018
    Inventors: Atsushi MIYAWAKI, Hiroyuki KATAYAMA
  • Patent number: 10030055
    Abstract: A mode of a polypeptide according to the present invention exhibits a fluorescence property, and has (1) an amino acid sequence represented by SEQ ID NO. 1 or NO. 2, (2) an amino acid sequence represented by SEQ ID NO. 1 or NO. 2 in which amino acid sequence 1 to 34 amino acids have been replaced or otherwise modified, (3) a sequence identity of 85% or more with respect to the amino acid sequence represented by SEQ ID NO. 1 or NO. 2, or (4) an amino acid sequence encoded by a polynucleotide that hybridizes under a stringent condition with a polynucleotide having a sequence complementary to a polynucleotide that encodes the polypeptide defined in (1).
    Type: Grant
    Filed: August 22, 2014
    Date of Patent: July 24, 2018
    Assignee: RIKEN
    Inventors: Atsushi Miyawaki, Ryoko Ando
  • Patent number: 9989518
    Abstract: This invention relates to a method for measuring autophagy in cells, comprising using, as a probe reagent, a single fluorescent protein, to measure a change in fluorescence properties of the fluorescent probe reagent depending on pH changes associated with autophagy, thereby determining the presence or activity of autophagy, wherein the single fluorescent protein is resistant to degrading enzyme activity in the lysosome or vacuole of the cell, it is not denatured or inactivated under acidic to neutral pH environment, and it is capable of changing excitation spectra or fluorescence spectra when located under the environments of acidic region and neutral region.
    Type: Grant
    Filed: October 8, 2010
    Date of Patent: June 5, 2018
    Assignee: Japan Science and Technology Agency
    Inventors: Atsushi Miyawaki, Hiroyuki Katayama
  • Patent number: 9957308
    Abstract: In order to provide a novel fluorescent protein and use thereof, the polypeptide according to the present invention has fluorescent properties in the presence of bilirubin and includes (1) the amino acid sequence of SEQ ID NO: 1, (2) an amino acid sequence having, for example, substitution of 1 to 21 amine acids in the amino acid sequence of SEQ ID NO: 1, (3) an amino acid sequence having 85% or more sequence identity to the amino acid sequence of SEQ ID NO: 1, or (4) the amino acid sequence encoded by a polynucleotide that hybridizes with a polynucleotide consisting of a sequence complementary to the polynucleotide encoding the polypeptide according to the amino acid sequence in (1) under a stringent condition.
    Type: Grant
    Filed: February 28, 2014
    Date of Patent: May 1, 2018
    Assignee: RIKEN
    Inventors: Atsushi Miyawaki, Akiko Kumagai
  • Publication number: 20180087014
    Abstract: Provided is a measurement method whereby the amount of unbound bilirubin (UB) can be exactly reflected whether a specimen contains a large amount of conjugated bilirubin or not. The measurement method for UB according to the present invention comprises decomposition step (i), decomposition stopping step (ii), contact step (iii) and detection step (iv). In decomposition step (i), a blood sample containing unconjugated bilirubin (iD-Bil) and conjugated bilirubin (D-Bil) is subjected to an oxidative decomposition reaction of UB in iD-Bil and D-Bil. In decomposition stopping step (ii), the oxidative decomposition reaction is stopped to give a decomposition product of the sample. In contact step (iii), the decomposition product of the sample is contacted with UnaG that is capable of specifically binding to iD-Bil. Separately, an unreacted sample, which is the blood sample not subjected to decomposition step (i), is contacted with UnaG too.
    Type: Application
    Filed: March 30, 2016
    Publication date: March 29, 2018
    Inventors: Sota IWATANI, Ichiro MORIOKA, Hajime NAKAMURA, Atsushi MIYAWAKI, Akiko KUMAGAI
  • Publication number: 20170370810
    Abstract: A clearing reagent in accordance with an embodiment of the present invention for making a biological material transparent is a solution containing: at least one compound selected from the group consisting of urea and a urea derivative; sorbitol; and a surfactant which is contained at a concentration of 5 (w/v) % or less.
    Type: Application
    Filed: January 20, 2016
    Publication date: December 28, 2017
    Inventors: Atsushi MIYAWAKI, Hiroshi HAMA
  • Publication number: 20160280746
    Abstract: A mode of a polypeptide according to the present invention exhibits a fluorescence property, and has (1) an amino acid sequence represented by SEQ ID NO. 1 or NO. 2, (2) an amino acid sequence represented by SEQ ID NO. 1 or NO. 2 in which amino acid sequence 1 to 34 amino acids have been replaced or otherwise modified, (3) a sequence identity of 85% or more with respect to the amino acid sequence represented by SEQ ID NO. 1 or NO. 2, or (4) an amino acid sequence encoded by a polynucleotide that hybridizes under a stringent condition with a polynucleotide having a sequence complementary to a polynucleotide that encodes the polypeptide defined in (1).
    Type: Application
    Filed: August 22, 2014
    Publication date: September 29, 2016
    Applicant: Riken
    Inventors: Atsushi MIYAWAKI, Ryoko ANDO
  • Patent number: 9249449
    Abstract: This invention relates to a probe reagent comprising, in order from the N-terminus to the C-terminus, the amino acid sequences of a fluorescent protein I, a peptide capable of terminating protein degradation (i.e., a degradation-terminating peptide), a spacer peptide, a fluorescent protein II, and a protein to be degraded, wherein the protein to be degraded is a protein degraded by the ubiquitin-proteasome system, and the probe reagent is degraded from the C-terminus, but that the degradation of the probe reagent is terminated at the degradation-terminating peptide, a nucleic acid encoding the probe reagent, and use of the probe reagent or the nucleic acid.
    Type: Grant
    Filed: January 21, 2011
    Date of Patent: February 2, 2016
    Assignees: Japan Science and Technology Agency, Riken
    Inventors: Atsushi Miyawaki, Masahiko Hirano
  • Publication number: 20160009771
    Abstract: In order to provide a novel fluorescent protein and use thereof, the polypeptide according to the present invention has fluorescent properties in the presence of bilirubin and includes (1) the amino acid sequence of SEQ ID NO: 1, (2) an amino acid sequence having, for example, substitution of 1 to 21 amine acids in the amino acid sequence of SEQ ID NO: 1, (3) an amino acid sequence having 85% or more sequence identity to the amino acid sequence of SEQ ID NO: 1, or (4) the amino acid sequence encoded by a polynucleotide that hybridizes with a polynucleotide consisting of a sequence complementary to the polynucleotide encoding the polypeptide according to the amino acid sequence in (1) under a stringent condition.
    Type: Application
    Filed: February 28, 2014
    Publication date: January 14, 2016
    Applicant: RIKEN
    Inventors: Atsushi MIYAWAKI, Akiko KUMAGAI
  • Publication number: 20150346196
    Abstract: An antibody composition, which is an aspect of the present invention, contains at least one compound selected from the group consisting of urea and urea derivatives, the compound being contained in the antibody composition at a concentration of not less than 0.1 M and less than 1 M, the antibody composition being a solution.
    Type: Application
    Filed: July 10, 2013
    Publication date: December 3, 2015
    Inventors: Atsushi Miyawaki, Hiroshi Hama