Patents by Inventor Atsushi Muraguchi
Atsushi Muraguchi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10533204Abstract: As a method for highly efficiently amplifying a TCR cDNA in a short period of time, there is provided a method for amplifying a T cell receptor (TCR) cDNA, which comprises the following step (1) and step (2): (1) the step of performing PCR by using at least one kind of the L primer mentioned below, the C primer 1 or UTR primer 1 mentioned below, and cDNA obtained from a single cell as the template to obtain an amplification product 1; an L primer of 30- to 60-nucleotide length comprising an adapter part of 15- to 25-nucleotide length, and a leader region-annealing part of 15- to 25-nucleotide length, which is ligated downstream from the adapter part, and can anneal to a part of a leader region containing a translation initiation codon, or an upstream part thereof, a C primer 1 of 15- to 25-nucleotide length, which can anneal to a part of a constant region, or a UTR primer 1 of 15- to 25-nucleotide length, which can anneal to a part of a 3? untranslated region; (2) the step of performing PCR by using the aType: GrantFiled: May 29, 2015Date of Patent: January 14, 2020Assignees: National University Corporatlon University of Toyama, SC World, Inc.Inventors: Hiroshi Hamana, Hiroyuki Kishi, Atsushi Muraguchi, Kiyomi Shitaoka
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Patent number: 9977017Abstract: Provided are a method and means permitting the simultaneous measurement of the reactive properties of more than 10,000 of antigen-stimulated lymphocytes being held on a chip and the separate determination of the states of individual cells. A microwell array comprises multiple wells and a coating layer on one of the principal surfaces of a base member, the wells being of a size permitting the entry of only a single cell into each well. A coating layer of a substance capable of binding to a substance produced by the cells contained in the wells is present on the principal surface around the wells.Type: GrantFiled: October 4, 2016Date of Patent: May 22, 2018Assignees: TOYAMA PREFECTURE, VALNEVAInventors: Aishun Jin, Hiroyuki Kishi, Atsushi Muraguchi, Tsutomu Obata
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Publication number: 20170183700Abstract: As a method for highly efficiently amplifying a TCR cDNA in a short period of time, there is provided a method for amplifying a T cell receptor (TCR) cDNA, which comprises the following step (1) and step (2): (1) the step of performing PCR by using at least one kind of the L primer mentioned below, the C primer 1 or UTR primer 1 mentioned below, and cDNA obtained from a single cell as the template to obtain an amplification product 1; an L primer of 30- to 60-nucleotide length comprising an adapter part of 15- to 25-nucleotide length, and a leader region-annealing part of 15- to 25-nucleotide length, which is ligated downstream from the adapter part, and can anneal to a part of a leader region containing a translation initiation codon, or an upstream part thereof, a C primer 1 of 15- to 25-nucleotide length, which can anneal to a part of a constant region, or a UTR primer 1 of 15- to 25-nucleotide length, which can anneal to a part of a 3? untranslated region; (2) the step of performing PCR by using the aType: ApplicationFiled: May 29, 2015Publication date: June 29, 2017Inventors: Hiroshi HAMANA, Hiroyuki KISHI, Atsushi MURAGUCHI, Kiyomi SHITAOKA
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Publication number: 20170166904Abstract: The purpose of the invention is to provide means with which it is possible to efficiently select a vector to which a foreign gene has been introduced when a foreign gene is to be introduced by homologous recombination to a vector having multiple sequences homologous with one another. The vector comprises, in succession, a replication origin, a sequence A, a marker gene X, two sequences C and D for introducing a foreign gene by homologous recombination, and a sequence B homologous with sequence A. The two sequences C and D are directly or indirectly adjacent to one another. The vector is used for introducing a foreign gene between the two adjacent sequences C and D.Type: ApplicationFiled: December 7, 2016Publication date: June 15, 2017Inventors: Masae HORII, Hiroyuki KISHI, Eiji KOBAYASHI, Tatsuhiko OZAWA, Atsushi MURAGUCHI
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Publication number: 20170023562Abstract: Provided are a method and means permitting the simultaneous measurement of the reactive properties of more than 10,000 of antigen-stimulated lymphocytes being held on a chip and the separate determination of the states of individual cells. A microwell array comprises multiple wells and a coating layer on one of the principal surfaces of a base member, the wells being of a size permitting the entry of only a single cell into each well. A coating layer of a substance capable of binding to a substance produced by the cells contained in the wells is present on the principal surface around the wells.Type: ApplicationFiled: October 4, 2016Publication date: January 26, 2017Inventors: Aishun JIN, Hiroyuki KISHI, Atsushi MURAGUCHI, Tsutomu OBATA
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Patent number: 9494575Abstract: Provided are a method and means permitting the simultaneous measurement of the reactive properties of more than 10,000 of antigen-stimulated lymphocytes being held on a chip. A microwell array comprises multiple wells capturing single cells and a coating layer on a principal surface around the wells containing a substance capable of binding to a substance produced by the cells in the wells. A method for screening a target cell comprises: causing specimen cells and a cell culture broth to be contained in the wells of the microwell array; culturing the cells in a state permitting the diffusion of substances from the wells into the coating layer; feeding a label substance binding specifically to a substance produced by a target cell onto the coating layer; and detecting the substance produced by the target cell by the label substance binding around the well to specify the target cell.Type: GrantFiled: August 1, 2008Date of Patent: November 15, 2016Assignees: TOYAMA PREFECTURE, VALNEVAInventors: Aishun Jin, Hiroyuki Kishi, Atsushi Muraguchi, Tsutomu Obata
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Patent number: 9310362Abstract: A microwell array chip made of silicon and having multiple microwells where each microwell is used to store a single specimen organic cell. The microwell of the array chip is of a size and shape to hold just one organic cell and the interior surface of the microwells are coated with a fluorocarbon film.Type: GrantFiled: April 18, 2011Date of Patent: April 12, 2016Assignee: VALNEVAInventors: Atsushi Muraguchi, Hiroyuki Kishi, Yoshiharu Tokimitsu, Sachiko Kondo, Tsutomu Obata, Satoshi Fujiki, Yoshiyuki Yokoyama, Hirofumi Nabesawa, Sotohiro Takabayashi, Katsumi Tanino
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Publication number: 20150203886Abstract: An object is to provide a TCR closing system that enables not only bias-free analysis of TCR repertoires, but also collection of antigen-specific TCR ?/? cDNA pairs and evaluation of functions thereof. There is provided a method for producing a gene of T cell receptor (TCR) specific to an antigen A, which comprises 1) the step of stimulating a group of T cells including a T cell specific to an antigen A or one T cell specific to an antigen A under a condition effective for amplification of a TCR gene; 2) the step of identifying a T cell specific to an antigen A among the group of T cells including a T cell specific to the antigen A, and sorting one T cell specific to the antigen A into a vessel; and 3) the step of subjecting the one activated T cell specific to the antigen A in the vessel to PCR to amplify a gene of TCR specific to the antigen A.Type: ApplicationFiled: July 24, 2013Publication date: July 23, 2015Inventors: Hiroyuki Kishi, Atsushi Muraguchi, Eiji Kobayashi, Tatsuhiko Ozawa
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Publication number: 20150038363Abstract: An object of the present invention is to stimulate a T cell without using a peptide/MHC tetramer. In the present invention, the step of supplying an antigen peptide to a T cell having a T cell receptor (TCR) that can recognize the antigen peptide on cell surface to form a complex of a major histocompatibility complex (MHC) molecule on the cell surface of the T cell and the antigen peptide is used, and the T cell is stimulated through recognition by TCR of the antigen peptide as the MHC molecule-antigen peptide complex on the cell surface of the same T cell. Such a stimulating and activating method would be applicable to not only T cells, but also various cells. According to the present invention, an antigen-specific T cell can be identified without establishing any antigen-specific T cell strain, and without using such a reagent as MHC/peptide tetramer. That is, a cancer-specific T cell can be efficiently and conveniently identified.Type: ApplicationFiled: March 6, 2013Publication date: February 5, 2015Inventors: Hiroyuki Kishi, Atsushi Muraguchi, Hiroshi Hamana, Eiji Kobayashi, Tatsuhiko Ozawa
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Publication number: 20140162320Abstract: The purpose of the invention is to provide means with which it is possible to efficiently select a vector to which a foreign gene has been introduced when a foreign gene is to be introduced by homologous recombination to a vector having multiple sequences homologous with one another. The vector comprises, in succession, a replication origin, a sequence A, a marker gene X, two sequences C and D for introducing a foreign gene by homologous recombination, and a sequence B homologous with sequence A. The two sequences C and D are directly or indirectly adjacent to one another. The vector is used for introducing a foreign gene between the two adjacent sequences C and D.Type: ApplicationFiled: March 27, 2012Publication date: June 12, 2014Inventors: Masae Horii, Hiroyuki Kishi, Eiji Kobayashi, Tatsuhikio Ozawa, Atsushi Muraguchi
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Patent number: 8664003Abstract: A chip useful for treating cells and the like which has a mechanism and a structure wherein the size of a hole pattern is arbitrarily changed so that cells can easily move in and get out from the hole in scattering or collecting cells but can hardly get out from the hole during washing or antigen-stimulation. The chip comprises a crosslinked product of a temperature-responsive polymer as a constituting member and being provided with a film having a hole pattern on the surface of a baseboard. A method of producing the chip comprises applying a composition containing a crosslinkable temperature-responsive polymer on the surface of a baseboard to thereby form a coating film, crosslinking the coating film to thereby form the crosslinked product as described above and then forming a hole pattern on the coating film of the crosslinked product.Type: GrantFiled: August 8, 2012Date of Patent: March 4, 2014Assignees: Toyama Prefecture, Nissan Chemical Industries, Ltd.Inventors: Eiichi Tamiya, Yoshiyuki Yokoyama, Satoshi Fujiki, Katsumi Tanino, Atsushi Muraguchi, Hiroyuki Kishi, Yoshiharu Tokimitsu, Shohei Yamamura
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Patent number: 8445193Abstract: A microwell array chip that has multiple microwells and is employed to contain a single lymphocyte specimen in each microwell and detect antigen-specific lymphocytes in single units; wherein the microwell array chip is of a shape and of dimensions where only one lymphocyte is contained in each microwell. A method of detecting antigen-specific lymphocytes comprising the steps of adding antigen to each microwell in the above microwell array chip, stimulating the lymphocyte specimen, and detecting lymphocyte specimens reacting with the antigen.Type: GrantFiled: December 17, 2008Date of Patent: May 21, 2013Assignee: VivalisInventors: Atsushi Muraguchi, Hiroyuki Kishi, Eiichi Tamiya, Masayasu Suzuki
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Publication number: 20120301942Abstract: A chip useful for treating cells and the like which has a mechanism and a structure wherein the size of a hole pattern is arbitrarily changed so that cells can easily move in and get out from the hole in scattering or collecting cells but can hardly get out from the hole during washing or antigen-stimulation. The chip comprises a crosslinked product of a temperature-responsive polymer as a constituting member and being provided with a film having a hole pattern on the surface of a baseboard. A method of producing the chip comprises applying a composition containing a crosslinkable temperature-responsive polymer on the surface of a baseboard to thereby form a coating film, crosslinking the coating film to thereby form the crosslinked product as described above and then forming a hole pattern on the coating film of the crosslinked product.Type: ApplicationFiled: August 8, 2012Publication date: November 29, 2012Applicants: TOYAMA PREFECTURE, NISSAN CHEMICAL INDUSTRIES, LTD.Inventors: Eiichi TAMIYA, Yoshiyuki YOKOYAMA, Satoshi FUJIKI, Katsumi TANINO, Atsushi MURAGUCHI, Hiroyuki KISHI, Yoshiharu TOKIMITSU, Shohei YAMAMURA
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Publication number: 20110294678Abstract: Provided are a method and means permitting the simultaneous measurement of the reactive properties of more than 10,000 of antigen-stimulated lymphocytes being held on a chip and the separate determination of the states of individual cells. A microwell array comprises multiple wells and a coating layer on one of the principal surfaces of a base member, the wells being of a size permitting the entry of only a single cell into each well. A coating layer of a substance capable of binding to a substance produced by the cells contained in the wells is present on the principal surface around the wells.Type: ApplicationFiled: August 1, 2008Publication date: December 1, 2011Applicants: SC World Inc., Toyama PrefectureInventors: Aishun Jin, Hiroyuki Kishi, Atsushi Muraguchi, Tsutomu Obata
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Publication number: 20110195496Abstract: The present invention is directed to a microwell array chip made of silicon and having multiple microwells. Each microwell is used to store a single specimen organic cell. Each microwell is of a size and shape holding just one organic cell, and the interior surface of the microwells are coated with a fluorocarbon film.Type: ApplicationFiled: April 18, 2011Publication date: August 11, 2011Inventors: Atsushi MURAGUCHI, Hiroyuki Kishi, Yoshiharu Tokimitsu, Sachiko Kondo, Tsutomu Obata, Satoshi Fujiki, Yoshiyuki Yokoyama, Hirofumi Nabesawa, Sotohiro Takabayashi, Katsumi Tanino
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Publication number: 20110129816Abstract: A device for detecting or quantifying influenza viruses in a sample, which comprises a detection region having a human anti-influenza virus nucleoprotein antibody immobilized onto a support, a sample supply region, and a sample-migrating region; and a kit for detecting or quantifying influenza viruses, which comprises a solid phase in which a human anti-influenza virus nucleoprotein antibody is fixed onto a carrier.Type: ApplicationFiled: June 5, 2009Publication date: June 2, 2011Applicants: NATIONAL UNIVERSITY CORPORATION UNIVERSITY OF TOYAMA, SC WORLD, INC., KYOWA MEDEX CO., LTD.Inventors: Atsushi Muraguchi, Hiroyuki Kishi, Tatsuhiko Ozawa, Mamoru Ikemoto, Hitoshi Ono, Kazuki Morita, Koji Uzawa
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Publication number: 20090181859Abstract: A microwell array chip that has multiple microwells and is employed to contain a single lymphocyte specimen in each microwell and detect antigen-specific lymphocytes in single units; wherein the microwell array chip is of a shape and of dimensions where only one lymphocyte is contained in each microwell. A method of detecting antigen-specific lymphocytes comprising the steps of adding antigen to each microwell in the above microwell array chip, stimulating the lymphocyte specimen, and detecting lymphocyte specimens reacting with the antigen.Type: ApplicationFiled: December 17, 2008Publication date: July 16, 2009Inventors: Atsushi MURAGUCHI, Hiroyuki Kishi, Eiichi Tamiya, Masayasu Suzuki
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Publication number: 20090130384Abstract: [Problems] To provide a novel chip useful for treating cells and the like which has a mechanism and a structure wherein the size of a hole pattern is arbitrarily changed so that cells can easily move in and get out from the hole in scattering or collecting cells but can hardly get out from the hole during washing or antigen-stimulation. [Means for Solving Problems] A chip comprising a crosslinked product of a temperature-responsive polymer as a constituting member and being provided with a film having a hole pattern on the surface of a baseboard. A method of producing a chip which comprises a crosslinked product of a temperature-responsive polymer as a constituting member and is provided with a film having a hole pattern on the surface of the baseboard.Type: ApplicationFiled: September 27, 2006Publication date: May 21, 2009Applicants: TOYAMA PREFECTURE, NISSAN CHEMICAL INDUSTRIES, LTD.Inventors: Eiichi Tamiya, Yoshiyuki Yokoyama, Satoshi Fujiki, Katsumi Tanino, Atsushi Muraguchi, Horoyuki Kishi, Yoshiharu Tokimitsu, Shohei Yamamura
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Publication number: 20080014631Abstract: A microwell array chip having multiple microwells on a principal surface of a substrate, said microwells being of a shape and size permitting the storage of only a single organic cell in each microwell, wherein microwell markers are present on the same substrate surface as the openings of the microwells. A microwell array chip having multiple microwells on a principal surface of a substrate, said microwells being of a shape and size permitting the storage of only a single organic cell in each microwell, wherein protrusions are present in the openings of said microwells so as to narrow said openings. A method for manufacturing the microwell array chip.Type: ApplicationFiled: September 22, 2004Publication date: January 17, 2008Applicants: Sachiko KONDO, Yoshiharu TOKIMITSUInventors: Atsushi Muraguchi, Hiroyuki Kishi, Yoshiharu Tokimitsu, Sachiko Kondo, Tsutomu Obata, Satoshi Fujiki, Yoshiyuki Yokoyama, Hirofumi Nabesawa, Sotohiro Takabayashi, Katsumi Tanino
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Publication number: 20060134704Abstract: A microwell array chip that has multiple microwells and is employed to contain a single lymphocyte specimen in each microwell and detect antigen-specific lymphocytes in single units; wherein the microwell array chip is of a shape and of dimensions where only one lymphocyte is contained in each microwell. A method of detecting antigen-specific lymphocytes comprising the steps of adding antigen to each microwell in the above microwell array chip, stimulating the lymphocyte specimen, and detecting lymphocyte specimens reacting with the antigen.Type: ApplicationFiled: September 30, 2003Publication date: June 22, 2006Inventors: Atsushi Muraguchi, Hiroyuki Kishi, Eiichi Tamiya, Masayasu Suzuki