Abstract: An object of the present invention is to provide a polypeptide having excellent alkaline stability and also allowing an immunoglobulin or a fragment thereof that has been bound to be eluted in a weakly acidic range, by modifying an amino acid sequence of an immunoglobulin-binding domain of Protein A. By substitution of serine at position 41 with an amino acid with a hydrophobic side chain, tyrosine, or histidine in each immunoglobulin-binding domain of Protein A, a polypeptide is obtained having avidity for an immunoglobulin or a polypeptide containing an Fc region thereof, having excellent alkaline stability, and also allowing the immunoglobulin or the polypeptide containing an Fc region thereof that has been bound to be efficiently eluted under weakly acidic mild conditions.

Abstract: An object of the present invention is to provide a polypeptide having a high binding capacity for an immunoglobulin kappa chain, and having excellent alkali stability, by modifying an amino acid sequence of an immunoglobulin-binding domain of Protein L derived from Peptostreptococcus magnus. A polypeptide having a high binding capacity for an immunoglobulin kappa chain, and having excellent alkali stability can be obtained by substituting specific lysine residues in an immunoglobulin-binding domain of Protein L derived from Peptostreptococcus magnus 3316 strain, with a basic amino acid or a hydroxyl group-containing amino acid.

Abstract: A multimeric immunoglobulin-binding protein having improved properties as an affinity ligand for affinity chromatography, and an insoluble support immobilizing such a multimer. The immunoglobulin-binding protein is represented by the formula: (R1)n-(R2)m, or (R2)m-(R1)n. R2 is an immunoglobulin-binding domain including an amino acid residue that covalently bonds to an insoluble support upon immobilization reaction with the insoluble support, and R1 is an immunoglobulin-binding domain without containing an amino acid residue the presence of which in the sequence compared to when it is absent in the sequence reduces the immunoglobulin-binding activity of the support yielded by the immobilization reaction. The immunoglobulin-binding protein satisfies: (1) n is an integer of 5 to 9; (2) m is an integer of 1 or 2; (3) the n (R1) domains may or may not have the same sequence; and (4) the total number of domains (n+m) is 6 to 10.

Abstract: An object of the present invention is to provide a polypeptide having a high binding capacity for an immunoglobulin kappa chain, and having excellent alkali stability, by modifying an amino acid sequence of an immunoglobulin-binding domain of Protein L derived from Peptostreptococcus magnus. A polypeptide having a high binding capacity for an immunoglobulin kappa chain, and having excellent alkali stability can be obtained by substituting specific lysine residues in an immunoglobulin-binding domain of Protein L derived from Peptostreptococcus magnus 3316 strain, with a basic amino acid or a hydroxyl group-containing amino acid.

Abstract: A multimeric immunoglobulin-binding protein having improved properties as an affinity ligand for affinity chromatography, and an insoluble support inmmobilizing such a multimer. The immunoglobulin-binding protein is represented by the formula: (R1)n-(R2)m, or (R2)m-(R1)n. R2 is an immunoglobulin-binding domain including an amino acid residue that covalently bonds to an insoluble support upon immobilization reaction with the insoluble support, and R1 is an immunoglobulin-binding domain without containing an amino acid residue the presence of which in the sequence compared to when it is absent in the sequence reduces the immunoglobulin-binding activity of the support yielded by the immobilization reaction. The immunoglobulin-binding protein satisfies: (1) n is an integer of 5 to 9; (2) m is an integer of 1 or 2; (3) the n (R1) domains may or may not have the same sequence; and (4) the total number of domains (n+m) is 6 to 10.

Abstract: Provided is affinity chromatography carrier for an immunoglobulin that simultaneously has high immunoglobulin-binding capability and chemical stability and that can be produced at low cost. An immunoglobulin-binding protein in which an amino acid substitution for not only maximizing the number of lysine residues on the protein surface of helix 3 and its periphery but also minimizing the number of lysine residues present on the protein surfaces of helix 1 and helix 2 as immunoglobulin-binding regions and/or an amino acid substitution for eliminating an aspartic acid-proline sequence have (has) been carried out, or a multimer thereof, is used as an affinity ligand for an affinity chromatography carrier.

Abstract: Provided is affinity chromatography carrier for an immunoglobulin that simultaneously has high immunoglobulin-binding capability and chemical stability and that can be produced at low cost. An immunoglobulin-binding protein in which an amino acid substitution for not only maximizing the number of lysine residues on the protein surface of helix 3 and its periphery but also minimizing the number of lysine residues present on the protein surfaces of helix 1 and helix 2 as immunoglobulin-binding regions and/or an amino acid substitution for eliminating an aspartic acid-proline sequence have (has) been carried out, or a multimer thereof, is used as an affinity ligand for an affinity chromatography carrier.

Abstract: A pharmaceutical composition for treating hyperlipidemia containing, as the active ingredient, at least one carboxamide compounds represented by the formula (1) which possess excellent activities in lowering serum lipid, as well as having low toxicity with high safety.Said carboxamide compounds represented by the formula (1) are known compounds developed by the present inventors, but the pharmacological activities for treating hyperlipidemia have not been found yet.

Abstract: In numerically controlling a machine tool having a plurality of movable members controlled by respective programs, the programs are automatically altered to reliably avoid collision between the movable members. Labor and time required for the operator to alter the programs are reduced.

Abstract: Novel carboxamide compounds represented by the general formula (I) possess excellent activities for lowering lipids and thus they are useful as agents for treating and preventing various diseases (hyperlipidemia) such as hypercholesterolemia, hypertriglyceridemia, hyperphospholipidemia, hyperlipacidemia, and the like.

Abstract: The method of the invention includes displaying a profile outline (POL) and a graphic cursor (CSR) on a display screen, adopting points, which are obtained on the display screen by successively positioning the graphic cursor and inputting its coordinates at starting points and end points of linear cutting or circular arc cutting, displaying a tool shape (TL) having a designated diameter at the inputted points, displaying a tool path (TLP) by successively connecting the inputted points by straight lines or circular arcs, and creating an NC program for moving a tool along the tool path to perform pocket machining.

Abstract: For reliably and easily judging whether or not a cutting locus is correct, which locus has been set in a cutting locus or tool path control program, which program has a step of commanding the rotational angle of a rotary table carrying a workpiece and a step of commanding the locus of a cutter corresponding to each rotational angle commanding step. A rotational angle range for displaying the locus of the cutter is set through a keyboard or the like. A CPU causes a display only if the locus of the cutter is within the set range.

Abstract: A conversational-type programming apparatus well suited for use when data are to be input in response to prompts on a conversational display in order to create a partial program, is provied. When a predetermined parameter item has been selected, the details of the selected item can be correctly understood, even by a person who is not skilled in NC programming language, thanks to a display of auxiliary graphics corresponding to the selected item. This enables an operator to input machining data while referring to auxiliary graphics corresponding to the parameter items, as a result of which the operator can rapidly become skilled at determining inputs.

Abstract: The present invention is to enable the locus of a tool or the like to be drawn on a display screen (25) based on an NC command program on an optimum scale and as quickly as possible.A microcomputer (10) obtains a magnification for image drawing and center coordinates of an image drawing space such that maximum and minimum coordinate values entered through a key input means (15) will be put in the display screen (25). Based upon such a magnification and center coordinates, the locus of a tool or the like is drawn on the display screen (25). In parallel with the above drawing process, the microcomputer (10) obtains maximum and minimum coordinate values of the NC command program.

Abstract: In a numerical control apparatus, tools to be used are sequentially read out from a tool order list in accordance with an order of tools to be used (S2, S9), and blocks read out from the source program and using the tools are searched (S3, S6). A new program is created in accordance with a designated tool order (S4), and machining commands are generated by the new program.

Abstract: A numerical control method, and a numerical control device for subjecting a workpiece to a predetermined machining operation on the basis of a machining program. When a specified signal is generated during a machining operation under the control of the machining program, a subprogram, to which the numerical control device responds to execute processing in accordance with the specified signal, is stored in memory beforehand. Machining in progress under the control of the machining program is interrupted temporarily by the generation of the specified signal. At the same time, the subprogram is read from memory and is utilized in the processing of predetermined process steps. Following such processing, machining under the control of the machining program is resumed from the point of interruption or from an earlier point.