Abstract: Disclosed is a highly efficient method for production of highly pure mutant-type human erythropoietin. The method is for production of mutant-type human erythropoietin, in which a transformed mammalian cell is allowed to produce the mutant-type human erythropoietin, and the supernatant of the culture is subjected to hydrophobic column chromatography, multimodal anion exchange column chromatography, anion exchange column chromatography, phosphate group affinity column chromatography, and gel filtration column chromatography, in this order.

Abstract: Disclosed is a method for production of recombinant human alpha-galactosidase A (rh alpha-Gal A) in a large scale, with a high purity. The method comprises the steps of (a) culturing rh alpha-Gal A-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to anion-exchange column chromatography, (d) to hydrophobic column chromatography, (e) to a column chromatography employing as solid phase a material having affinity for phosphate group, (f) to cation-exchange column chromatography, (g) to dye-affinity column chromatography, and (h) to gel filtration column chromatography, in the order.

Abstract: Disclosed is a highly efficient method for production of highly pure mutant-type human erythropoietin. The method is for production of mutant-type human erythropoietin, in which a transformed mammalian cell is allowed to produce the mutant-type human erythropoietin, and the supernatant of the culture is subjected to hydrophobic column chromatography, multimodal anion exchange column chromatography, anion exchange column chromatography, phosphate group affinity column chromatography, and gel filtration column chromatography, in this order.

Abstract: Disclosed is a method for production of recombinant human alpha-galactosidase A (rh alpha-Gal A) in a large scale, with a high purity. The method comprises the steps of (a) culturing rh alpha-Gal A-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to anion-exchange column chromatography, (d) to hydrophobic column chromatography, (e) to a column chromatography employing as solid phase a material having affinity for phosphate group, (f) to cation-exchange column chromatography, (g) to dye-affinity column chromatography, and (h) to gel filtration column chromatography, in the order.

Abstract: Disclosed is a method for production of recombinant human alpha-galactosidase A (rh alpha-Gal A) in a large scale, with a high purity. The method comprises the steps of (a) culturing rh alpha-Gal A-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to anion-exchange column chromatography, (d) to hydrophobic column chromatography, (e) to a column chromatography employing as solid phase a material having affinity for phosphate group, (f) to cation-exchange column chromatography, and (g) to gel filtration column chromatography, in the order.

Abstract: Disclosed is a method for production of recombinant human iduronate 2-sulfatase (rhI2S) (the 26th to 550th amino acids of SEQ ID NO: 10) in a large scale, with a high purity, and mannose 6-phosphate residues. The method comprises the steps of (a) culturing rhI2S (the 26th to 550th amino acids of SEQ ID NO: 10)-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to cation-exchange column chromatography, (d) to dye affinity column chromatography, (e) to anion-exchange column chromatography, and (f) to a column chromatography employing as solid phase a material having affinity for phosphate group, and (g) to gel filtration column chromatography, in the order.

Abstract: Disclosed is a method for production of recombinant human alpha-galactosidase A (rh alpha-Gal A) in a large scale, with a high purity. The method comprises the steps of (a) culturing rh alpha-Gal A-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to anion-exchange column chromatography, (d) to hydrophobic column chromatography, (e) to a column chromatography employing as solid phase a material having affinity for phosphate group, (f) to cation-exchange column chromatography, and (g) to gel filtration column chromatography, in the order.

Abstract: Disclosed is an efficient method for production of aminopeptidase. The method comprises either transforming host bacteria with an aminopeptidase gene and with a neutral protease gene, or transforming some part of host bacteria with an aminopeptidase gene while transforming the other part of the host bacteria with a neutral protease gene, culturing in a medium the hose bacteria transformed with the aminopeptidase gene and with the neutral protease gene, or culturing a mixture of the host bacteria transformed with the aminopeptidase gene and the host bacteria transformed with the neutral protease gene, to let both the aminopeptidase and the neutral protease be expressed, and collecting the aminopeptidase thus produced from the culture mixture.

Abstract: Disclosed is a method for production of recombinant human iduronate 2-sulfatase (rhI2S) in a large scale, with a high purity, and mannose 6-phosphate residues. The method comprises the steps of (a) culturing rhI2S-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to cation-exchange column chromatography, (d) to dye affinity column chromatography, (e) to anion-exchange column chromatography, and (f) to a column chromatography employing as solid phase a material having affinity for phosphate group, and (g) to gel filtration column chromatography, in the order.

Abstract: Disclosed is a method for production of recombinant human FSH in high yield and a high purity. The method comprises the steps of: (a) culturing recombinant human FSH-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to cation-exchange column chromatography, (d) dye affinity column chromatography, (e) hydrophobic column chromatography, and (f) gel filtration column chromatography to collect recombinant human FSH-active fractions, in the order.

Abstract: A field effect transistor includes an active region provided in a projecting part on a surface of a semiconductor substrate, the projecting part extending in a fixed direction parallel to the surface, and a gate electrode provided on a sidewall of the projecting part along the fixed direction with a gate insulating films interposed.

Abstract: Disclosed is a method for production of recombinant human FSH in high yield and a high purity. The method comprises the steps of: (a) culturing recombinant human FSH-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to cation-exchange column chromatography, (d) dye affinity column chromatography, (e) hydrophobic column chromatography, and (f) gel filtration column chromatography to collect recombinant human FSH-active fractions, in the order.

Abstract: The electronic control unit includes a control section monitoring a manipulation of an ignition switch of a vehicle and a timer section for automatically starting up said control section. The electronic control unit is provided with a fault diagnosis function of monitoring, by use of the control section, a state of an electric load connected to said electronic control unit and supplied with electric power from the vehicle battery when the main relay is in the on state after the control section outputs the stop command, and diagnosing whether or not the main relay is in a fault state where the main relay cannot be controlled from the on state to the off state on the basis of monitored state of the electric load.

Abstract: The electronic control unit includes a control section monitoring a manipulation of an ignition switch of a vehicle, and a timer section for automatically starting up the control section. The electronic control unit is provided with a fault diagnosis function of monitoring, by use of the control section being supplied with electric power through a main relay, a time elapsed since the control section outputs a stop command commanding the main relay to change to the off state, and diagnosing that the main relay is in a fault state where the main relay cannot be controlled to the off state when the monitored time exceeds a threshold time predetermined on the basis of a response time needed for the main relay to change from the on state to the off state in response to the stop command.

Abstract: The electronic control unit includes a control section monitoring a manipulation of an ignition switch of a vehicle, and a timer section for automatically starting up the control section. The electronic control unit is provided with a fault diagnosis function of monitoring, by use of the control section being supplied with electric power through a main relay, a time elapsed since the control section outputs a stop command commanding the main relay to change to the off state, and diagnosing that the main relay is in a fault state where the main relay cannot be controlled to the off state when the monitored time exceeds a threshold time predetermined on the basis of a response time needed for the main relay to change from the on state to the off state in response to the stop command.

Abstract: The electronic control unit includes a control section monitoring a manipulation of an ignition switch of a vehicle and a timer section for automatically starting up said control section. The electronic control unit is provided with a fault diagnosis function of monitoring, by use of the control section, a state of an electric load connected to said electronic control unit and supplied with electric power from the vehicle battery when the main relay is in the on state after the control section outputs the stop command, and diagnosing whether or not the main relay is in a fault state where the main relay cannot be controlled from the on state to the off state on the basis of monitored state of the electric load.

Abstract: In step 400, microcomputer 12 calculates a temperature change amount ?T1 of ignition coil FC caused by the heat generating from the ignition coil FC, based on a previous calculated temperature T(n?1) of ignition coil FC and an engine rotational speed. In step 410, the microcomputer 12 calculates a temperature change amount ?T2 of ignition coil FC caused by the heat received from the engine, based on the previous calculated temperature T(n?1) of ignition coil FC and a cooling water temperature of the engine. In step 420, the microcomputer 12 calculates a temperature change amount ?T3 of ignition coil FC caused by the heat released to the outside, based on the previous calculated temperature T(n?1) of ignition coil FC and an intake air temperature of the engine. Then, in step 430, the microcomputer 12 calculates a present ignition coil temperature T(n) based on these change amounts ?T1, ?T2, and ?T3.

Abstract: In step 400, microcomputer 12 calculates a temperature change amount ?T1 of ignition coil FC caused by the heat generating from the ignition coil FC, based on a previous calculated temperature T(n?1) of ignition coil FC and an engine rotational speed. In step 410, the microcomputer 12 calculates a temperature change amount ?T2 of ignition coil FC caused by the heat received from the engine, based on the previous calculated temperature T(n?1) of ignition coil FC and a cooling water temperature of the engine. In step 420, the microcomputer 12 calculates a temperature change amount ?T3 of ignition coil FC caused by the heat released to the outside, based on the previous calculated temperature T(n?1) of ignition coil FC and an intake air temperature of the engine. Then, in step 430, the microcomputer 12 calculates a present ignition coil temperature T(n) based on these change amounts ?T1, ?T2, and ?T3.

Abstract: In an ECU for vehicles, a microcomputer operates with a main power from a battery, and a clock IC operates with sub power from the battery and measures time continuously irrespective of whether the microcomputer is operating. The microcomputer stores time data of the clock IC just before the supply of main power is turned off, and calculates a soak time or engine stop period from the stored time data and a current time data of the clock IC only after the supply of main power is turned on again and engine cranking is completed. This soak time represents the engine stop period. Operation of a cooling water temperature sensor or the like is checked by using the sensor output in relation to the calculated soak time.

Abstract: In an ECU for vehicles, a clock IC operates with sub power and measures time continuously irrespective of whether a microcomputer is operating. The microcomputer determines whether the clock IC has been reset on the basis of a history indicating that the sub power has fallen below a data holding voltage of an SRAM which also operates on the sub power. Alternatively, the microcomputer determines whether the clock IC has been reset by checking data held in the SRAM. The microcomputer determines failure of a water temperature sensor from a soak time calculated from time data from the clock IC and a detection value of the water temperature sensor on restarting of the engine. When the clock IC has been reset, the microcomputer prohibits this failure determination of the water temperature sensor.