Patent number: 5403728
Abstract: The invention refers to a new microbial process for preparing .beta.,.delta.-dihydroxy-7-[1,2,6,7,8,8a-hexahydro-2.6-dimethyl-8-(2-methy l-butyryloxy)-naphthalen-1-yl]-heptanoic acid .delta.-lactone of formula (I) and .beta.,.delta.-dihydroxy-7-[1,2,6,7,8,8a-hexahydro-2.6-dimethyl-8-(2-methy lbutyryloxy)-naphtalen-1-yl]-heptanoic acid of formula (II), ##STR1## by the aerobic fermentation of the submerged culture of an imperfect fungus strain biosynthesizing the above compounds, in a nutrient medium containing utilizable carbon and nitrogen sources as well as mineral salts, and by isolating the product of formula (I), which comprises cultivating a strain of the new Aspergillus obscurus fungus species producing the above compound(s) of formulas (I) and/or (II), in a temperature range of 25.degree. to 30.degree. C. and, if desired, separating the product(s) formed from the fermentation broth, then isolating it in the lactone form of formula (I) and, if desired, purifying the same.
Type:
Grant
Filed:
June 17, 1993
Date of Patent:
April 4, 1995
Assignee:
BIOGAL Gyogyszergyar RT
Inventors:
Antonia Jekkel, Eva Ilkoy, Istvan M. Szabo, Gabor Ambrus, Attila Andor, Ilona Varga, Imre Moravcsik, Istvan Szabo, Janos Erdei, Kalman Polya, Andras Kiss, Laszlo Cseke, Karoly Nagy, Mihaly Kaszas, Lajos Kiss, Istvan Magyi, Edit Halasz, Gyorgy Santha
Patent number: 5156960
Abstract: The invention relates to a microbial process for the production of immunosuppressive cyclosporine antibiotic complex or of its components, cyclosporine A, cyclosporine B and cyclosporine C, by the aerobic fermentation of a filamentous fungus strain biosynthesizing the above antibiotic(s) in a nutrient medium containing utilizable carbon and nitrogen sources as well as mineral salts, and by isolating the products formed, which comprises culturing a strain of the novel Tolypocladium varium fungus species producing the cyclosporine antibiotic complex, preferably tolypocladium varium sp. nov. CY/93, deposited at the National Collection of Agricultural and Industrial Microorganisms, Budapest, Hungary under the number NCAIM(P)F 001005, on a nutrient medium containing carbon sources, organic and inorganic nitrogen sources as well as mineral salts, under aerobic conditions, at 25.degree. to 30.degree. C., and, if desired, isolating and purifying the cyclosporine antibiotic complex or its components produced.
Type:
Grant
Filed:
December 20, 1989
Date of Patent:
October 20, 1992
Assignee:
Biogal Gyogyszergyar
Inventors:
Antonia Jekkel nee Bokany, Gabor Ambrus, Eva Toth-Sarudy, Istvan Mihaly, Agota Hulber nee Dobos, Attila Andor, Karoly Albrecht, Kalman Konczol, Valeria Szell, Eva Tomori nee Joszt, Imre Moravcsik, Kalman Polya, Janos Erdei, Lajos Kiss, Bela Makadi, Karoly Nagy, Bela Palotas, Etelka Deli nee Konszky, Karoly Buzasi, Aniko Molnar nee Antal, Gyorgy Santha, Vilma Szaszhegyesi
