Abstract: The present invention relates to a method for detecting and/or enumerating a microorganism or a group of microorganisms of interest comprising a) contacting, in a container, a sample suspected of containing said microorganism or group of microorganisms with a solid growth medium containing nutrients to support growth of said microorganism or group of microorganisms and with at least one fluorescent dye; b) incubating said container under conditions and for a time sufficient to form microcolonies of said microorganism or group of microorganisms; and c) detecting and/or enumerating microcolonies emitting a fluorescent signal of said at least one fluorescent dye, thereby detecting, identifying and/or enumerating said microorganism or group of microorganisms contained in the sample.

Abstract: The present invention relates to a method, and related device, for observing, and where appropriate detecting and optionally enumerating, microbiological objects on a support, such as a membrane or a solid growth medium. In particular, said method comprises a) illuminating an area of a support with at least two incident collimated light sources forming an angle (?) of at least 10° with respect to the normal of the support, b) acquiring an image of said area of said support illuminated by said at least two incident collimated light sources by means of a light receiving element having its optical acquisition axis along the normal of the support, and c) detecting the presence or the absence of microbiological objects on said area of said support by discrimination, on said acquired image, of light reflected, scattered and/or diffused from the support and from microbiological objects on said support.

Abstract: The present invention concerns a method for labeling specifically living bacteria of a given category of bacteria in a sample comprising bacteria, the method comprising the steps of: a) incubating said bacteria of said sample with at least one analog of a monosaccharide compound, said monosaccharide being an endogenous monosaccharide residue of glycans of the outer membrane of such given category of bacteria, the said endogenous monosaccharide residue comprising an ulosonic acid or ulosonate salt residue, the said analog of a monosaccharide compound being a modified monosaccharide substituted at a given position by a first reactive chemical group capable to react with a second reactive group of a labeling molecule, the said given position being preferably a position which comprises a free group in the said endogenous monosaccharide residue incorporated within said glycans of the outer membrane of the bacteria, b) contacting said bacteria with a said labeling molecule comprising a said second reactive group

Abstract: A kit for labeling bacteria, the kit including: an analog of an endogenous monosaccharide residue of glycans of the outer membrane of a given category of bacteria, the endogenous monosaccharide residue being an ulosonic acid or ulosonate salt residue, the analog of a monosaccharide compound being a modified monosaccharide substituted at a given position by a first reactive group capable of reacting with a second reactive group of a labeling molecule; the labeling molecule having the second reactive group capable of reacting with said first reactive group; reactants for generating the reaction of the first reactive group of the analog residue incorporated within the glycans of the outer membrane of the bacteria with the second reactive group of the labeling molecule; and a culture or incubation medium allowing growth of the given category of bacteria, specific to the growth of the given category of bacteria.

Abstract: A kit for labeling bacteria, the kit including: an analog of an endogenous monosaccharide residue of glycans of the outer membrane of a given category of bacteria, the endogenous monosaccharide residue being an ulosonic acid or ulosonate salt residue, the analog of a monosaccharide compound being a modified monosaccharide substituted at a given position by a first reactive group capable of reacting with a second reactive group of a labeling molecule; the labeling molecule having the second reactive group capable of reacting with said first reactive group; reactants for generating the reaction of the first reactive group of the analog residue incorporated within the glycans of the outer membrane of the bacteria with the second reactive group of the labeling molecule; and a culture or incubation medium allowing growth of the given category of bacteria, specific to the growth of the given category of bacteria.

Abstract: The present invention concerns a method for labeling specifically living bacteria of a given category in a sample, the method including: a) incubating said bacteria of said sample with at least one analog of a monosaccharide compound, and b) contacting the bacteria with a labeling molecule having a second reactive group.

Abstract: The present invention concerns a method for labeling specifically living bacteria of a given category in a sample, the method including: a) incubating said bacteria of said sample with at least one analog of a monosaccharide compound, and b) contacting the bacteria with a labeling molecule having a second reactive group.