Abstract: The present invention provides a rapid and precise test for objectively determining a bacterium species. The assay is applicable to sample isolated from blood, serum, other bodily fluids or environmental samples. The detection method comprises the steps of annealing a region in a DNA template to a specific oligonucleotide primer set comprising SEQ-FOR and SEQ-REV in a complimentary fashion, said primer set designed to give rise to a PCR product having a predetermined size dictated by a complimentary primer set; amplifying said region of DNA template by performing a polymerase chain reaction to produce said PCR product; determining a nucleatide sequence of said PCR product; and comparing said PCR product to a DNA sequence in a database to determine a species of bacterium.

Abstract: The present invention provides methods for detecting the presence of invasive pathogenic molds in biological samples that are based on amplification of mold nucleic acids. The methods may further comprise quantitating and real time detection of the invasive mold. The methods of the invention are highly specific and do not co-amplify human or other yeast nucleic acids. The methods of the invention are also extremely sensitive. Thus, methods for diagnosing infections caused by invasive mold are provided. The invention also provides kits for detection of invasive molds.

Abstract: The present invention provides methods for detecting the presence of pathogenic moulds in biological samples that are based on amplification of mould nucleic acids. The methods may further comprise quantitating and real time detection of the mould. The methods of the invention are highly specific and do not co-amplify human or other yeast nucleic acids. The methods of the invention are also extremely sensitive. Thus, methods for diagnosing infections caused by mould are provided. The invention also provides kits for detection of moulds.