Abstract: A system for selecting CRISPR guides for knocking out one or more target genes in a target cell from a multiplicity of candidate guides comprises a memory and a processor. The processor determines whether the candidate guide meets a plurality of thresholds. The thresholds are associated with: a transcript support level; targeting a consensus sequence of a target gene; which exon of the target gene is targeted; targeting of a primary transcript, targeting of a common isoform; a precomputed prediction of editing outcomes; mapping to an expressed sequence; fraction of gene expression attributable to targeted transcripts; a common SNP overlap threshold; which exon of the target gene is targeted; overlap of a selected guide; predicted frameshift percentage; maximum and minimum GC content; off target score; where a coding sequence is targeted. In response to meeting the thresholds, the processor selects the candidate guide as a selected CRISPR guide.

Abstract: Evaluating an effect of one or more perturbations on cells of a first cell type is described. One method includes obtaining a screen definition for a screen, where the screen includes a cell-based assay that is run on a temporarily contiguous basis using a plurality of multi-well plates. The method includes obtaining control vectors including measurements of corresponding features of cells in control wells. The method includes obtaining test vectors including measurements of corresponding features of cells in test wells. The method includes forming a variability model based on a variance across the of control vectors, and embedding test vectors onto the variability model, thereby obtaining a set of variability model values. The method includes using the set of variability model values to resolve an effect of at least one data perturbation in the plurality of data perturbations on the first cell type.

Abstract: Provided herein are methods of manufacturing viruses. Also provided herein are bioreactors comprising virus-infected host cells comprising viruses. In some aspects, the present disclosure relates to production of recombinant oncolytic viruses, e.g., recombinant vaccinia viruses.

Abstract: In an embodiment of a rotational sensor, a multi-pole magnet assembly comprises multiple magnets configured to rotate about a rotational axis of the rotational sensor, where the multi-pole magnet assembly is in a first plane perpendicular to the rotational axis. The magnetic sensor is arranged in a second plane also perpendicular to the rotational axis and in proximity to the multi-pole magnet assembly. Each magnet of the multiple magnets has poles aligned parallel to the rotational axis and perpendicular to the first plane of the magnetic sensor. In another embodiment, the magnetic sensor is arranged at a first radius away from the rotational axis, whereas the multiple magnets of the magnet assembly are arranged at a second radius, not equal to the first radius, away from the rotational axis. In yet another embodiment, a rotational sensor comprises a housing and rotatable shaft upon which the magnet assembly is mounted.

Abstract: In an embodiment of a rotational sensor, a multi-pole magnet assembly comprises multiple magnets configured to rotate about a rotational axis of the rotational sensor, where the multi-pole magnet assembly is in a first plane perpendicular to the rotational axis. The magnetic sensor is arranged in a second plane also perpendicular to the rotational axis and in proximity to the multi-pole magnet assembly. Each magnet of the multiple magnets has poles aligned parallel to the rotational axis and perpendicular to the first plane of the magnetic sensor. In another embodiment, the magnetic sensor is arranged at a first radius away from the rotational axis, whereas the multiple magnets of the magnet assembly are arranged at a second radius, not equal to the first radius, away from the rotational axis. In yet another embodiment, a rotational sensor comprises a housing and rotatable shaft upon which the magnet assembly is mounted.