Abstract: A computer-implemented method of training a neural network to improve a characteristic of a protein comprises collecting a set of amino acid sequences from a database, compiling each amino acid sequence into a three-dimensional crystallographic structure of a folded protein, training a neural network with a subset of the three-dimensional crystallographic structures, identifying, with the neural network, a candidate residue to mutate in a target protein, and identifying, with the neural network, a predicted amino acid residue to substitute for the candidate residue, to produce a mutated protein, wherein the mutated protein demonstrates an improvement in a characteristic over the target protein. A system for improving a characteristic of a protein is also described. Improved blue fluorescent proteins generated using the system are also described.

Abstract: A computer-implemented method of training a neural network to improve a characteristic of a protein comprises collecting a set of amino acid sequences from a database, compiling each amino acid sequence into a three-dimensional crystallographic structure of a folded protein, training a neural network with a subset of the three-dimensional crystallographic structures, identifying, with the neural network, a candidate residue to mutate in a target protein, and identifying, with the neural network, a predicted amino acid residue to substitute for the candidate residue, to produce a mutated protein, wherein the mutated protein demonstrates an improvement in a characteristic over the target protein. A system for improving a characteristic of a protein is also described. Improved blue fluorescent proteins generated using the system are also described.

Abstract: A computer-implemented method of training a neural network to improve a characteristic of a protein comprises collecting a set of amino acid sequences from a database, compiling each amino acid sequence into a three-dimensional crystallographic structure of a folded protein, training a neural network with a subset of the three-dimensional crystallographic structures, identifying, with the neural network, a candidate residue to mutate in a target protein, and identifying, with the neural network, a predicted amino acid residue to substitute for the candidate residue, to produce a mutated protein, wherein the mutated protein demonstrates an improvement in a characteristic over the target protein. A system for improving a characteristic of a protein is also described. Improved blue fluorescent proteins generated using the system are also described.

Abstract: A wrench head having a jaw with jaw members having interior side faces and throat support surfaces defining an interior for mateably receiving a nut or bolt head. Extending from the top side of each jaw member and the top side of a handle connection portion and overhanging the interior defined by the interior side faces of the jaw members and throat support surfaces is a wrench head overhang, that, when placed over a nut or bolt head, is in contact with the top side of the bolt head or top side of the nut. The wrench head overhang serves to retain the wrench head over the nut or bolt head so that the wrench head cannot slip down off of the nut or bolt head. The overhang is dimensioned so as to that when the wrench of the present invention is used to rotate a nut that has been threaded on a bolt shaft, the bolt shaft will clear the sides of the overhang.

Abstract: A wrench head having a jaw with jaw members having interior side faces and throat support surfaces defining an interior for mateably receiving a nut or bolt head. Extending from the top side of each jaw member and the top side of a handle connection portion and overhanging the interior defined by the interior side faces of the jaw members and throat support surfaces is a wrench head overhang, that, when placed over a nut or bolt head, is in contact with the top side of the bolt head or top side of the nut. The wrench head overhang serves to retain the wrench head over the nut or bolt head so that the wrench head cannot slip down off of the nut or bolt head. The overhang is dimensioned so as to that when the wrench of the present invention is used to rotate a nut that has been threaded on a bolt shaft, the bolt shaft will clear the sides of the overhang.