Abstract: Lipid-based vesicles, typically herein called transfection competent vesicles (TCVs), configured to safely and efficiently deliver DNA, RNA, other nucleic acid and protein cargoes into target cells. The safety and efficiency are each, and both, achieved in part by eliminating organic solvents such as ethanol and detergents such as sodium dodecyl sulfate from the TCV loading processes (i.e., inserting a cargo into the TCV), TCV storage processes, and/or TCV delivery processes. The cargoes can also comprise nucleic acids complexed with a protein, such as a ribonucleoprotein (RNP). The systems, compositions, devices and methods, etc., herein, in some embodiments, can provide empty TCVs that can if desired be loaded at the bench without use of specialized equipment.

Abstract: Lipid-based vesicles, typically herein called transfection competent vesicles (TCVs), configured to safely and efficiently deliver DNA, RNA, other nucleic acid and protein cargoes into target cells. The safety and efficiency are each, and both, achieved in part by eliminating organic solvents such as ethanol and detergents such as sodium dodecyl sulfate from the TCV loading processes (i.e., inserting a cargo into the TCV), TCV storage processes, and/or TCV delivery processes. The cargoes can also comprise nucleic acids complexed with a protein, such as a ribonucleoprotein (RNP). The systems, compositions, devices and methods, etc., herein, in some embodiments, can provide empty TCVs that can if desired be loaded at the bench without use of specialized equipment.

Abstract: This disclosure relates to an environmental compliance system installed onboard a ship. The environmental compliance system includes a computing device that records and requests authorization for overboard discharge when discharge occurs within a defined geofence. The system receives data from the ship automation system including ship location and operational valve opening commands made to discharge effluent overboard and into the surrounding water. Before the operational valve opening command can be received by the valve, the compliance box confirms ship position and compares against programmed no-discharge geofence or a plurality of geofences. When the ship is inside a no-discharge geofence, the computing device may request a manual override authorization from the responsible duty holder before completing the command so as to prevent an accidental unintended discharge. Data is securely stored by the compliance box and may also be transmitted to external cloud-based data receivers.

Abstract: Lipid-based vesicles, typically herein called transfection competent vesicles (TCVs), configured to safely and efficiently deliver DNA, RNA, other nucleic acid and protein cargoes into target cells. The safety and efficiency are each, and both, achieved in part by eliminating organic solvents such as ethanol and detergents such as sodium dodecyl sulfate from the TCV loading processes (i.e., inserting a cargo into the TCV), TCV storage processes, and/or TCV delivery processes. The cargoes can also comprise nucleic acids complexed with a protein, such as a ribonucleoprotein (RNP). The systems, compositions, devices and methods, etc., herein, in some embodiments, can provide empty TCVs that can if desired be loaded at the bench without use of specialized equipment.

Abstract: A hand controller that includes a control member mounted on a hand held base for commanding a remote vehicle or movement of a virtual object within a virtual environment includes an input device mounted for generating tilt commands for a sensor mounted on the remote vehicle or the virtual object, the input device being located on a front of the base and oriented to be displaced up and down to tilt the sensor or the virtual point of view when the base is held during operation.

Abstract: A hand controller that includes a control member mounted on a hand held base for commanding a remote vehicle or movement of a virtual object within a virtual environment includes an input device mounted for generating tilt commands for a sensor mounted on the remote vehicle or the virtual object, the input device being located on a front of the base and oriented to be displaced up and down to tilt the sensor or the virtual point of view when the base is held during operation.

Abstract: A hand controller that includes a control member mounted on a hand held base for commanding a remote vehicle or movement of a virtual object within a virtual environment includes an input device mounted for generating tilt commands for a sensor mounted on the remote vehicle or the virtual object, the input device being located on a front of the base and oriented to be displaced up and down to tilt the sensor or the virtual point of view when the base is held during operation.

Abstract: A biogas blending and energy content verification system and method for controlled enhancement of a biogas feedstock stream energy content profile by selective sampling and analysis of the biogas feedstock stream and controlled injection of a refined gas of a known, higher energy content into the biogas feedstock stream to produce a blended biogas having an augmented energy content profile meeting or exceeding a pre-established minimum to meet end user requirements.

Abstract: A hand controller that includes a control member mounted on a hand held base for commanding a remote vehicle or movement of a virtual object within a virtual environment includes an input device mounted for generating tilt commands for a sensor mounted on the remote vehicle or the virtual object, the input device being located on a front of the base and oriented to be displaced up and down to tilt the sensor or the virtual point of view when the base is held during operation.

Abstract: An anti-fouling, radiation emitter isolation assembly includes an adapter element mounted to a fixed port at the top of a leachate tank. A radiation emitter/detector is further mounted to the adapter element for transmitting radiation through the fixed port to determine a fluid level. The adapter element incorporates an isolation valve that when opened provides an unobstructed window through the fixed port for the transmitted radiation and when closed isolates said radiation emitter/detector from the fixed port. In particular, selectively isolating the radiation emitter/detector from the interior of the tank permits removal of the radiation emitter/detector from the adapter element without requiring a shutdown of leachate tank system operations.

Abstract: A biogas blending and energy content verification system and method for controlled enhancement of a biogas feedstock stream energy content profile by selective sampling and analysis of the biogas feedstock stream and controlled injection of a refined gas of a known, higher energy content into the biogas feedstock stream to produce a blended biogas having an augmented energy content profile meeting or exceeding a pre-established minimum to meet end user requirements.

Abstract: Embodiments of the invention include systems and methods for providing gripper recovery using a magazine drop-out feature. Embodiments operate in context of a data storage library having a number of media cartridges physically located within slots of one or more magazines. A robotic hand assembly uses a gripper mechanism to retrieve and ferry the cartridges between the magazines and one or more media drives. In some instances, the gripper mechanism is unable to disengage from the cartridge or to disengage the cartridge from its magazine slot. Embodiments detect this condition and direct the robotic hand assembly to drop out of engagement with the cartridge through the drop-out region of the magazine slot, allowing the gripper mechanism to recover.

Abstract: Embodiments of the invention include systems and methods for providing gripper recovery using a magazine drop-out feature. Embodiments operate in context of a data storage library having a number of media cartridges physically located within slots of one or more magazines. A robotic hand assembly uses a gripper mechanism to retrieve and ferry the cartridges between the magazines and one or more media drives. In some instances, the gripper mechanism is unable to disengage from the cartridge or to disengage the cartridge from its magazine slot. Embodiments detect this condition and direct the robotic hand assembly to drop out of engagement with the cartridge through the drop-out region of the magazine slot, allowing the gripper mechanism to recover.