Patents by Inventor Austin Payne
Austin Payne has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10900032Abstract: A system and method for automated single cell capture and processing is described, where the system includes a deck supporting and positioning a set of sample processing elements; a gantry for actuating tools for interactions with the set of sample processing elements supported by the deck; and a base supporting various processing subsystems and a control subsystems in communication with the processing subsystems. The system can automatically execute workflows associated with single cell processing, including mRNA capture, cDNA synthesis, protein-associated assays, and library preparation, for next generation sequencing.Type: GrantFiled: May 5, 2020Date of Patent: January 26, 2021Assignee: Bio-Rad Laboratories, Inc.Inventors: Kalyan Handique, Austin Payne, Sida Wang, Patrick Michael Tegels, William T. Koederitz, Daniel Genord, Grey Parker, Brian Boniface, Katlyn Curtin Mehne, Alec William Hitchiner
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Publication number: 20210003496Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.Type: ApplicationFiled: September 18, 2020Publication date: January 7, 2021Inventors: Kalyan Handique, Austin Payne, Vishal Sharma, Kyle Gleason, Priyadarshini Gogoi, Karthik Ganesan, Brian Boniface, Will Chow
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Publication number: 20200353459Abstract: A system and method for target material retrieval and processing, the system comprising: an adaptor configured to interface with a capture region of a capture substrate for capturing particles in single-particle format within a set of wells, wherein the adaptor comprises a first region configured to interface with the capture region, a second region, and a cavity extending from the first region to the second region; and a support structure coupled to the adaptor and providing a set of operation modes for movement of the adaptor relative to the capture substrate. The system enables methods for magnetic and/or other force-based methods of retrieval of target material (e.g., derived from single cells).Type: ApplicationFiled: June 19, 2020Publication date: November 12, 2020Inventors: Kalyan Handique, Swati Ranade, Vishal Sharma, Austin Payne, Sam Tuck
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Publication number: 20200354715Abstract: A system and method for automated single cell capture and processing is described, where the system includes a deck supporting and positioning a set of sample processing elements; a gantry for actuating tools for interactions with the set of sample processing elements supported by the deck; and a base supporting various processing subsystems and a control subsystems in communication with the processing subsystems. The system can automatically execute workflows associated with single cell processing, including mRNA capture, cDNA synthesis, protein-associated assays, and library preparation, for next generation sequencing.Type: ApplicationFiled: May 5, 2020Publication date: November 12, 2020Inventors: Kalyan Handique, Austin Payne, Sida Wang, Patrick Michael Tegels, Will Koederitz, Daniel Genord, Grey Parker, Brian Boniface, Katlyn Curtin Mehne, Alec William Hitchiner
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Publication number: 20200353472Abstract: A system and method for automated single cell capture and processing is described, where the system includes a deck supporting and positioning a set of sample processing elements; a gantry for actuating tools for interactions with the set of sample processing elements supported by the deck; and a base supporting various processing subsystems and a control subsystems in communication with the processing subsystems. The system can automatically execute workflows associated with single cell processing, including mRNA capture, cDNA synthesis, protein-associated assays, and library preparation, for next generation sequencing.Type: ApplicationFiled: May 5, 2020Publication date: November 12, 2020Inventors: Kalyan Handique, Austin Payne, Sida Wang, Patrick Michael Tegels, Will Koederitz, Daniel Genord, Grey Parker, Brian Boniface, Katlyn Curtin Mehne, Alec William Hitchiner
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Patent number: 10791140Abstract: Computer-implemented methods are provided for assessing the cybersecurity state of entities based on computer network characterization. The exemplary method can include obtaining, for one or more computer networks of a plurality of computer networks associated with an entity, a network dataset including a service set identifier (SSID); and obtaining a plurality of Internet Protocol (IP) addresses associated with the entity. The method can further include determining whether each of the plurality of computer networks includes a public network or a private network based on the network dataset; and assessing a cybersecurity state of the entity based on an evaluation of security characteristics of the IP addresses attributed to the private network, excluding security characteristics of the IP addresses attributed to the public network.Type: GrantFiled: January 29, 2020Date of Patent: September 29, 2020Assignee: BitSight Technologies, Inc.Inventors: Daniel Dahlberg, Austin Payne Allshouse
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Publication number: 20200230604Abstract: A system and method for isolating and analyzing single cells, wherein the system includes: an array of wells defined at a substrate, each well including an open surface and a well cavity configured to capture cells in one of a single-cell format and single-cluster format, and a fluid delivery module including a fluid reservoir superior to the array of wells through which fluid flow is controlled along a fluid path in a direction parallel to the broad face of the substrate; and wherein the method includes: capturing a population of non-cell particles into the array of wells in single-particle format; releasing, from the non-cell particles, a set of probes into the array of wells; capturing a population of cells into the array of wells in single-cell format; releasing biomolecules from each captured cell into the array of wells; and generating a set of genetic complexes comprising the biomolecules associated with a single captured cell and a subset of probes within individual wells of the array of wells.Type: ApplicationFiled: February 13, 2020Publication date: July 23, 2020Inventors: Kalyan Handique, Vishal Sharma, Priyadarshini Gogoi, William Chow, Austin Payne, Kyle Gleason, Brian Boniface, John Connolly, Sam Tuck
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Publication number: 20200132592Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.Type: ApplicationFiled: January 2, 2020Publication date: April 30, 2020Inventors: Kalyan Handique, Austin Payne, Vishal Sharma, Kyle Gleason, Priyadarshini Gogoi, Karthik Ganesan, Brian Boniface, Will Chow
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Patent number: 10564090Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.Type: GrantFiled: August 2, 2019Date of Patent: February 18, 2020Assignee: Celsee Diagnostics, Inc.Inventors: Kalyan Handique, Austin Payne, Vishal Sharma, Kyle Gleason, Priyadarshini Gogoi, Karthik Ganesan, Brian Boniface, Will Chow
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Publication number: 20190353579Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.Type: ApplicationFiled: August 2, 2019Publication date: November 21, 2019Inventors: Kalyan Handique, Austin Payne, Vishal Sharma, Kyle Gleason, Priyadarshini Gogoi, Karthik Ganesan, Brian Boniface, Will Chow
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Patent number: 10466160Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.Type: GrantFiled: November 16, 2017Date of Patent: November 5, 2019Assignee: Celsee Diagnostics, Inc.Inventors: Kalyan Handique, Austin Payne, Vishal Sharma, Kyle Gleason, Priyadarshini Gogoi, Karthik Ganesan, Brian Boniface, Will Chow
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Publication number: 20190270085Abstract: A system for isolating cells in at least one of single-cell format and single-cluster format, comprising a reservoir, including a reservoir inlet and a reservoir outlet, configured to receive a biological sample and to receive at least one fluid, a manifold configured to receive and deliver the biological sample and the at least one fluid from the reservoir into a biological sample substrate, the manifold comprising a broad surface comprising a central region configured to receive the biological sample substrate, a set of openings configured to enable fluid flow transmission across the biological sample substrate, a manifold inlet configured to transmit flow from the reservoir the first subset of openings, a manifold outlet configured at a downstream end of the broad surface and coupled to the second subset of openings, the manifold outlet configured to transmit waste fluid from the manifold.Type: ApplicationFiled: May 22, 2019Publication date: September 5, 2019Inventors: Kalyan Handique, Kyle Gleason, Austin Payne, Priyadarshini Gogoi, Christopher Siemer, Yi Zhou, Saedeh Javdani Sepehri
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Patent number: 10391492Abstract: A system and method for isolating and analyzing single cells, wherein the system includes: an array of wells defined at a substrate, each well including an open surface and a well cavity configured to capture cells in one of a single-cell format and single-cluster format, and a fluid delivery module including a fluid reservoir superior to the array of wells through which fluid flow is controlled along a fluid path in a direction parallel to the broad face of the substrate; and wherein the method includes: distributing a population of cells and a population of non-cell particles across the array of wells through the fluid reservoir to increase capture efficiency of individual cell-particle pairs within the array of wells, and processing the captured cell-particle pairs at the set of wells.Type: GrantFiled: August 28, 2018Date of Patent: August 27, 2019Assignee: Celsee Diagnostics, Inc.Inventors: Kalyan Handique, Vishal Sharma, Priyadarshini Gogoi, William Chow, Austin Payne, Kyle Gleason, Brian Boniface, John Connolly, Sam Tuck
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Patent number: 10350601Abstract: A system for isolating cells in at least one of single-cell format and single-cluster format, comprising a reservoir, including a reservoir inlet and a reservoir outlet, configured to receive a biological sample and to receive at least one fluid, a manifold configured to receive and deliver the biological sample and the at least one fluid from the reservoir into a biological sample substrate, the manifold comprising a broad surface comprising a central region configured to receive the biological sample substrate, a set of openings configured to enable fluid flow transmission across the biological sample substrate, a manifold inlet configured to transmit flow from the reservoir the first subset of openings, a manifold outlet configured at a downstream end of the broad surface and coupled to the second subset of openings, the manifold outlet configured to transmit waste fluid from the manifold.Type: GrantFiled: October 12, 2017Date of Patent: July 16, 2019Assignee: Celsee Diagnostics, Inc.Inventors: Kalyan Handique, Kyle Gleason, Austin Payne, Priyadarshini Gogoi, Christopher Siemer, Yi Zhou, Saedeh Javdani Sepehri
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Publication number: 20190064168Abstract: A system and method for isolating and analyzing single cells, wherein the system includes: an array of wells defined at a substrate, each well including an open surface and a well cavity configured to capture cells in one of a single-cell format and single-cluster format, and a fluid delivery module including a fluid reservoir superior to the array of wells through which fluid flow is controlled along a fluid path in a direction parallel to the broad face of the substrate; and wherein the method includes: distributing a population of cells and a population of non-cell particles across the array of wells through the fluid reservoir to increase capture efficiency of individual cell-particle pairs within the array of wells, and processing the captured cell-particle pairs at the set of wells.Type: ApplicationFiled: August 28, 2018Publication date: February 28, 2019Inventors: Kalyan Handique, Vishal Sharma, Priyadarshini Gogoi, William Chow, Austin Payne, Kyle Gleason, Brian Boniface, John Connolly, Sam Tuck
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Publication number: 20180088023Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.Type: ApplicationFiled: November 16, 2017Publication date: March 29, 2018Inventors: Kalyan Handique, Austin Payne, Vishal Sharma, Kyle Gleason, Priyadarshini Gogoi, Karthik Ganesan, Brian Boniface, Will Chow
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Patent number: 9925538Abstract: A system and method for capturing and analyzing cells comprising: a fluid delivery module; a reservoir configured to receive a biological sample including a target cell population and at least one fluid from the fluid delivery module; a manifold configured to receive and distribute the biological sample and at least one fluid from the reservoir into a cell capture device; a waste chamber configured to couple to the manifold; and a pump configured to couple to the waste chamber. In embodiments of the system configured to promote further purification of captured cells, the system can further comprise a magnet that enables further separation of captured cells from undesired sample materials. The system can additionally further comprise a heater configured to heat at least one fluid and/or biological sample, and a cell capture device configured to couple to the manifold, in order to facilitate capture of the target cell population.Type: GrantFiled: September 29, 2017Date of Patent: March 27, 2018Assignee: DeNovo Sciecnes, Inc.Inventors: Kalyan Handique, Kyle Gleason, Austin Payne, Priyadarshini Gogoi, Saedeh Sepehri Javdani, Christopher Siemer, Yi Zhou
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Publication number: 20180036731Abstract: A system for isolating cells in at least one of single-cell format and single-cluster format, comprising a reservoir, including a reservoir inlet and a reservoir outlet, configured to receive a biological sample and to receive at least one fluid, a manifold configured to receive and deliver the biological sample and the at least one fluid from the reservoir into a biological sample substrate, the manifold comprising a broad surface comprising a central region configured to receive the biological sample substrate, a set of openings configured to enable fluid flow transmission across the biological sample substrate, a manifold inlet configured to transmit flow from the reservoir the first subset of openings, a manifold outlet configured at a downstream end of the broad surface and coupled to the second subset of openings, the manifold outlet configured to transmit waste fluid from the manifold.Type: ApplicationFiled: October 12, 2017Publication date: February 8, 2018Inventors: Kalyan Handique, Kyle Gleason, Austin Payne, Priyadarshini Gogoi, Christopher Siemer, Yi Zhou, Saedeh Javdani Sepehri
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Publication number: 20180021782Abstract: A system and method for capturing and analyzing cells comprising: a fluid delivery module; a reservoir configured to receive a biological sample including a target cell population and at least one fluid from the fluid delivery module; a manifold configured to receive and distribute the biological sample and at least one fluid from the reservoir into a cell capture device; a waste chamber configured to couple to the manifold; and a pump configured to couple to the waste chamber. In embodiments of the system configured to promote further purification of captured cells, the system can further comprise a magnet that enables further separation of captured cells from undesired sample materials. The system can additionally further comprise a heater configured to heat at least one fluid and/or biological sample, and a cell capture device configured to couple to the manifold, in order to facilitate capture of the target cell population.Type: ApplicationFiled: September 29, 2017Publication date: January 25, 2018Inventors: Kalyan Handique, Kyle Gleason, Austin Payne, Priyadarshini Gogoi, Saedeh Sepehri Javdani, Christopher Siemer, Yi Zhou
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Patent number: 9821311Abstract: A system for isolating cells in at least one of single-cell format and single-cluster format, comprising a reservoir, including a reservoir inlet and a reservoir outlet, configured to receive a biological sample and to receive at least one fluid, a manifold configured to receive and deliver the biological sample and the at least one fluid from the reservoir into a biological sample substrate, the manifold comprising a broad surface comprising a central region configured to receive the biological sample substrate, a set of openings configured to enable fluid flow transmission across the biological sample substrate, a manifold inlet configured to transmit flow from the reservoir the first subset of openings, a manifold outlet configured at a downstream end of the broad surface and coupled to the second subset of openings, the manifold outlet configured to transmit waste fluid from the manifold.Type: GrantFiled: February 14, 2017Date of Patent: November 21, 2017Assignee: DeNovo Sciences, Inc.Inventors: Kalyan Handique, Kyle Gleason, Austin Payne, Priyadarshini Gogoi, Christopher Siemer, Yi Zhou, Saedeh Javdani Sepehri