Patents by Inventor Avrameas Stratis

Avrameas Stratis has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Non-radioactive labelled single-stranded probe, method for manufacturing it and method for detecting a specified nucleotide sequence using this probe
    Patent number: 4978749
    Abstract: The invention relates to a new probe for detecting a specified nucleic acid sequence. It consists of a recombinant DNA containing an insert complementary to the nucleic acid sequence to be detected and nuclecic acid sequences derived from the DNA (+) of phage M13, the bases which participate in the composition of this recombinant DNA, in particular the thymine groups, being replaced by 5-bromouracil groups. The invention also relates to a method for manufacturing this probe, this method comprising the culturing of cell hosts, more especially E. coli, which are auxotrophic for thymine in a medium containing a limiting concentration of thymine and containing 5-bromouracil. After the requisite incubation time, the supernatant is removed and the probe is recovered from the bacterial cells.
    Type: Grant
    Filed: July 11, 1989
    Date of Patent: December 18, 1990
    Assignees: Institute Pasteur, Centre National de la Recherche Scientifique
    Inventors: Avrameas Stratis, Hiroshi Sakamoto, Francois Traincard, Tuyen Vo Quang, Jean-Luc Guesdon, Therese Ternynck