Abstract: A screen frame transmission method includes a transmitting end capturing screen frame data and audio data and transmitting the same to a receiving end, and detecting for a user's operation and outputting a mode switching signal; a receiving end buffering the screen frame data transmitted by the transmitting end, and according to the mode switching signal, switching to the operating mode or the video mode, wherein during the video mode, a longer playback period of the screen frame data are buffered, and during the operating mode, a shorter playback period of the screen frame data are buffered. According to the aforementioned method, during the video mode, the video may be played back smoothly and the video and the audio are synchronized; during the operating mode, a low latency delay control experience may be provided. A receiving device and screen frame transmission system realizing the above method are also disclosed.