Abstract: A micro-electrode array is described comprising an insulating substrate and a plurality of conductive paths defined therein, each of which includes an electrode area with microscopic dimensions, a connection area with macroscopic dimensions, and a buried interconnection region, wherein the insulating substrate is made of a first polymeric material, such as PDMS, and the conductive paths are made of a second polymeric material, such as PEDOT. Furthermore, a method of manufacturing such micro-electrode array is described, based on the configuration of an insulating substrate volume by replica molding on the first polymeric material through a prearranged master mould, bearing a configuration of microstructures adapted to define a plurality of cavities; successively filling the cavities with a second conductive polymeric material so as to constitute the electrode areas, the connection areas, and the interconnection regions; and arranging an insulating material sealing layer for the filled cavities.