Abstract: The present disclosure provides a standardized and virus-free rapid propagation method of Dianthus caryophyllus, belonging to the technical field of asexual propagation. The method includes the following steps: conducting virus detection on a Dianthus caryophyllus explant, and inoculating the explant into an induction medium for induction culture; after the explant germinates, cutting buds, and determining whether a virus-free treatment is required according to a virus detection result, and continuing cultivating the buds; after cultivating to obtain cluster buds, inoculating the cluster buds into an expanding propagation medium for expanding propagation, and conducting the virus detection to eliminate a virus-carrying material; conducting expanding propagation on a virus-free material to a certain base, and transferring the virus-free material to a rooting medium for rooting culture.