Abstract: The present invention provides a culture method of cells and/or tissues including culturing cells and/or tissues in a suspended state by using a medium composition having an effect of preventing sedimentation of cells and/or tissues, which is afforded by substantially retaining the cells and/or tissues, without substantially increasing the viscosity of the solution, by nanofibers which have been added to the solution and uniformly dispersed in the liquid medium, and the like.

Abstract: The present invention aims to provide a novel compound capable of promoting cell proliferation in a cell culture (particularly three-dimensional cell culture). The hydrazide compound represented by the formula (I); wherein each symbol is as defined in DESCRIPTION, or a salt thereof, and a composition containing same can remarkably promote cell proliferation, sphere formation, cyst formation and/or organoid formation, and can also remarkably inhibit the activities of kinase such as LATS1, LATS2 and the like.

Abstract: The present invention provides a medium composition containing deacylated gellan gum or a salt thereof, and an acidic polysaccharide or a salt thereof capable of maintaining a random coil state in a divalent metal cation medium and cross-linking via a divalent metal ion, and permitting culture of a cell or a tissue in suspension, wherein a concentration of the deacylated gellan gum or a salt thereof in the medium composition is 0.002-0.01 (w/v) %, a concentration of the acidic polysaccharide or a salt thereof is 0.004-0.1 (w/v) %, and a mass ratio of the acidic polysaccharide or a salt thereof to the deacylated gellan gum or a salt thereof is not less than 1. In addition, the present invention provides a method for isolating a cell or tissue from a culture preparation containing the medium composition and cell or tissue, including applying a shear force to the culture preparation.

Abstract: The present invention aims to provide a novel kinase inhibitor and the like, and a therapeutic agent for a disease, a drug discovery screening method and the like utilizing such inhibitor and the like. The compound represented by the following formula (I) and a salt thereof can inhibit plural kinases including LATS (particularly LATS2) which is the major kinase in the Hippo signal transduction pathway. In addition, diseases or tissue damage associated with failure of cellular proliferation can be treated. Therefore, the present invention is beneficial, for example, in the research field of cell functions and diseases, in which the Hippo signal transduction pathway is involved, and the like. Furthermore, it is beneficial in the medical field for the treatment of such diseases and the like. wherein each symbol is as defined in the DESCRIPTION.

Abstract: The invention provides a coating agent for a flow passage which comprises a copolymer containing a recurring unit which contains an organic group of the formula (a) wherein Ua1 and Ua2 are defined herein, and a recurring unit which contains an organic group of the formula (b) wherein Ub1, Ub2, Ub3, and An? are defined herein. The invention also provides a flow passage device having the coating agent for a flow passage on at least part of an inner surface of a flow passage, a platelet-producing flow passage device and a method for manufacturing the same.

Abstract: The invention provides a ligand-bonded fiber in which a ligand having affinity for a cell membrane receptor is immobilized on a fiber precursor, and a cell culture substrate capable of repeating ex vivo amplification of a cell expressing a cell membrane receptor by using the ligand-bonded fiber.

Abstract: The present invention provides a medium additive composition containing a compound represented by the following formula (I), or a salt thereof: {wherein each symbol is as defined in the DESCRIPTION.

Abstract: The present invention provides a culture method of cells and/or tissues including culturing cells and/or tissues in a suspended state by using a medium composition having an effect of preventing sedimentation of cells and/or tissues, which is afforded by substantially retaining the cells and/or tissues without substantially increasing the viscosity of the solution by nanofibers which have been added to the solution and uniformly dispersed in the liquid medium, and the like.

Abstract: The present invention provides a production method of a medium composition, including previously removing divalent metal cations contained in a polymer compound having an anionic functional group, preparing a dry powder of the polymer compound, and mixing the dry powder and the medium, and the like. The present invention also provides a purification method of a polymer compound having an anionic functional group, which includes mixing a suspension of the polymer compound having an anionic functional group in water and a cation exchanger that exchanges a divalent metal cation with a monovalent metal cation to give a polymer compound having an anionic functional group, which shows a reduced amount of contaminating divalent metal cations.

Abstract: The present invention provides a culture method of cells and/or tissues including culturing cells and/or tissues in a suspended state by using a medium composition having an effect of preventing sedimentation of cells and/or tissues, which is afforded by substantially retaining the cells and/or tissues without substantially increasing the viscosity of the solution by nanofibers which have been added to the solution and uniformly dispersed in the liquid medium, and the like.

Abstract: The present invention provides a method of isolating cells or tissues from a culture preparation of the cells or tissues in a medium composition which enables culture of the cells or tissues in suspension, which comprises at least one step selected from the group consisting of the following (A), (B) and (C): (A) passing the culture preparation through a filter having fine pores having a pore diameter of 5-500 ?m, (B) adding a chelator to the culture preparation, and (C) diluting the culture preparation with a physiological aqueous solution.

Abstract: The present invention provides a medium composition containing deacylated gellan gum or a salt thereof, and an acidic polysaccharide or a salt thereof capable of maintaining a random coil state in a divalent metal cation medium and cross-linking via a divalent metal ion, and permitting culture of a cell or a tissue in suspension, wherein a concentration of the deacylated gellan gum or a salt thereof in the medium composition is 0.002-0.01 (w/v) %, a concentration of the acidic polysaccharide or a salt thereof is 0.004-0.1 (w/v) %, and a mass ratio of the acidic polysaccharide or a salt thereof to the deacylated gellan gum or a salt thereof is not less than 1. In addition, the present invention provides a method for isolating a cell or tissue from a culture preparation containing the medium composition and cell or tissue, including applying a shear force to the culture preparation.

Abstract: The invention provides a coating agent for a flow passage which comprises a copolymer containing a recurring unit which contains an organic group of the formula (a) wherein Ua1 and Ua2 are defined herein, and a recurring unit which contains an organic group of the formula (b) wherein Ub1, Ub2, Ub3, and An? are defined herein. The invention also provides a flow passage device having the coating agent for a flow passage on at least part of an inner surface of a flow passage, a platelet-producing flow passage device and a method for manufacturing the same.

Abstract: The invention provides a ligand-bonded fiber in which a ligand having affinity for a cell membrane receptor is immobilized on a fiber precursor, and a cell culture substrate capable of repeating ex vivo amplification of a cell expressing a cell membrane receptor by using the ligand-bonded fiber.

Abstract: The present invention provides a method of isolating cells or tissues from a culture preparation of the cells or tissues in a medium composition which enables culture of the cells or tissues in suspension, which comprises at least one step selected from the group consisting of the following (A), (B) and (C): (A) passing the culture preparation through a filter having fine pores having a pore diameter of 5-500 ?m, (B) adding a chelator to the culture preparation, and (C) diluting the culture preparation with a physiological aqueous solution.

Abstract: The present invention provides a culture method of cells and/or tissues including culturing cells and/or tissues in a suspended state by using a medium composition having an effect of preventing sedimentation of cells and/or tissues, which is afforded by substantially retaining the cells and/or tissues without substantially increasing the viscosity of the solution by nanofibers which have been added to the solution and uniformly dispersed in the liquid medium, and the like.

Abstract: The present invention provides a production method of a medium composition, including previously removing divalent metal cations contained in a polymer compound having an anionic functional group, preparing a dry powder of the polymer compound, and mixing the dry powder and the medium, and the like. The present invention also provides a purification method of a polymer compound having an anionic functional group, which includes mixing a suspension of the polymer compound having an anionic functional group in water and a cation exchanger that exchanges a divalent metal cation with a monovalent metal cation to give a polymer compound having an anionic functional group, which shows a reduced amount of contaminating divalent metal cations.