Abstract: A technique that can detect a target substance in a sample with higher sensitivity and at lower cost than a conventional technique is provided, which is a target substance detection method including: in a first reaction field, forming a complex by sandwiching a target substance between a first trapping substance immobilized on a solid phase and a second trapping substance labeled with the labeling substance, and separating a moiety including the labeling substance from the complex and moving it through a liquid filled in a tubular channel by a centrifugal force; and detecting the moiety including the labeling substance by light scattered by the moiety including the labeling substance.

Abstract: Provided is a target substance detection method that includes: forming a complex in a first reaction field, by sandwiching a target substance between a first trapping substance immobilized on a solid phase and a second trapping substance labeled with a labeling substance; separating a moiety that includes the labeling substance from the complex; moving the moiety to a second reaction field; and detecting the target substance by a signal based on the labeling substance in the second reaction field. As a result of this technology, a target substance in a sample can be detected with greater sensitivity and at a lower cost than when using conventional technology.