Abstract: Using a biosensor including a flow path into which blood containing a target is introduced, an upstream electrode pair disposed in the flow path and having a reagent reacting with the target disposed on either of the electrodes, and a downstream electrode pair disposed at a downstream side of the flow path; the method includes a first detection step of detecting a first introduction of blood as far as the upstream electrode pair; a second detection step of detecting a second introduction of blood as far as the downstream electrode pair; a time measurement step of measuring a time difference from the first introduction to the second introduction; and a measurement step of acquiring the value related to the target based on the time difference by using any two electrodes among the upstream and downstream electrode pair after the second introduction is detected.

Abstract: There is provided a biosensor for measuring a measuring target substance in a sample comprising an insulating substrate, an electrode pair provided on the insulating substrate, and a reagent that includes an oxidoreductase and an electron transfer substance and is provided at least on a working electrode of the electrode pair, wherein the working electrode is composed of one or more kinds of nickel alloys selected from the group consisting of a nickel-ruthenium alloy, a nickel-tungsten alloy, and a nickel-vanadium alloy.

Abstract: A measuring method includes applying a first signal, which is a direct current signal, having a first value to a sample in a flow passage using a first electrode and a fourth electrode which is different from the first, second, and third electrodes, measuring a first electric response value, applying a second signal having a second value higher than the first value to the sample using the second and third electrodes, measuring a second electric response value, and correcting a value obtained from the first electric response value based on the second electric response value.

Abstract: A measuring method includes applying a first signal having a first value to a sample disposed in the flow passage by using a working electrode and a counter electrode, measuring a first electric response value of the sample to the first signal, applying contiguously for a given time a second signal having a second value higher than the first value to the sample disposed in the flow passage by using the working electrode and the counter electrode, measuring a second electric response value of the sample within the given time to the second signal, the second electric response value indicating amount of electric charge generated by electrolysis of water in the sample, and correcting a value obtained from the first electric response value based on the second electric response value.