Abstract: In some embodiments, the systems and methods of the disclosure can provide high-throughput, programmable, and fully automatized tissue and/or cell culture and analysis platforms. In some embodiments, a culture analysis system may include a culture device that includes a cover configured to be secured to a main body, which may include one or more chambers. The cover may include one or more regions that overlaps with the one or more chambers of the main body when the cover is secured to a main body so that each region corresponds to a chamber of the main body. The cover may also include a fluidic pathway disposed in each region and configured be in fluidic communication with a corresponding chamber. Each fluidic pathway may include a fluid inlet and a fluid outlet disposed in each region. The cover may also include an optical pathway disposed in each region for the corresponding chamber.