Abstract: A ladder seat assembly for assuming a seated position on a ladder includes a first bracket and a second bracket that are each positionable on a respective step of the ladder. A pole is coupled to and extends downwardly from the second bracket to engage the first bracket. A seat mount is positionable around the pole such that the seat mount is centrally positioned between the first bracket and the second bracket when each of the first bracket and the second bracket are positioned on the ladder. A seat unit is removably attachable to the seat mount wherein the seat unit is configured to have the user sit thereon while the user is on the ladder thereby enhancing comfort for the user.