Abstract: A non-contact cell manipulation system comprises a supporting member, a micro-positioner, a probe holder having a proximal end and a distal end, the proximal end connected to the micro-positioner, a probe adapter removably connected to the distal end of the probe holder and a non-contact multiphysics probe fluidly and electrically connected to the probe adapter, wherein the probe includes at least one electrode, at least one aperture, and wherein the probe is configured to utilize electropermealization and electroheating in combination with hydrodynamic flow confinement to perform non-contact cell manipulation. A non-contact multiphysics probe and non-contact cell manipulation method are also disclosed.