Patents by Inventor Ayoub Rashtchian
Ayoub Rashtchian has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20210017575Abstract: Method and compositions for improving DNA polymerase and reverse transcriptase reactions are provided. Addition of anti-foam reagents to the reactions improves fluid handling, especially of small volumes and allows enhanced accuracy of optical detection, without substantially inhibiting enzymatic activity.Type: ApplicationFiled: August 11, 2020Publication date: January 21, 2021Inventors: Ayoub Rashtchian, David M. Schuster
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Publication number: 20190024167Abstract: The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.Type: ApplicationFiled: June 28, 2018Publication date: January 24, 2019Inventors: Irina Nazarenko, Ayoub Rashtchian, Joseph Solus, Richard M. Pires, Marlene Darfler, Gulilat Gebeyehu, Mekbib Astatke
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Patent number: 10041117Abstract: The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.Type: GrantFiled: July 11, 2014Date of Patent: August 7, 2018Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Irina Nazarenko, Ayoub Rashtchian, Joseph Solus, Richard M. Pires, Marlene Darfler, Gulilat Gebeyehu, Mekbib Astatke
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Patent number: 9988660Abstract: Methods for making eDNA molecules, for amplification of RNA by PCR and for preparation of cDNA libraries are provided. Kits for making eDNA molecules also are provided. Compositions are also provided comprising mixtures of reagents, including reverse transcriptases, buffers, cofactors and other components, suitable for immediate use in conversion of RNA into eDNA and RT PCR without dilution or addition of further components. These compositions are useful, alone or in the form of kits, for eDNA synthesis or nucleic acid amplification (e.g., by the Polymerase Chain Reaction) or for any procedure utilizing reverse transcriptases in a variety of research, medical, diagnostic, forensic and agricultural applications.Type: GrantFiled: October 8, 2015Date of Patent: June 5, 2018Assignee: QUANTA BIOSCIENCESInventors: Ayoub Rashtchian, David Schuster
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Patent number: 9873901Abstract: The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (RT-PCR). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step RT-PCR procedure using combinations of reverse transcriptase and thermostable DNA polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin. The invention thus facilitates the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of RNA molecules. The invention also is useful in the rapid production and amplification of cDNAs which may be used for a variety of industrial, medical and forensic purposes.Type: GrantFiled: September 26, 2014Date of Patent: January 23, 2018Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Jun Lee, Ayoub Rashtchian
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Patent number: 9556466Abstract: The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (RT-PCR). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step RT-PCR procedure using combinations of reverse transcriptase and thermostable DNA polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin. The invention thus facilitates the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of RNA molecules. The invention also is useful in the rapid production and amplification of cDNAs which may be used for a variety of industrial, medical and forensic purposes.Type: GrantFiled: February 11, 2013Date of Patent: January 31, 2017Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Jun Lee, Ayoub Rashtchian
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Publication number: 20160108466Abstract: The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.Type: ApplicationFiled: September 18, 2015Publication date: April 21, 2016Inventors: IRINA NAZARENKO, AYOUB RASHTCHIAN
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Publication number: 20160046975Abstract: Methods for making cDNA molecules, for amplification of RNA by PCR and for preparation of cDNA libraries are provided. Kits for making cDNA molecules also are provided. Compositions are also provided comprising mixtures of reagents, including reverse transcriptases, buffers, cofactors and other components, suitable for immediate use in conversion of RNA into cDNA and RT PCR without dilution or addition of further components. These compositions are useful, alone or in the form of kits, for cDNA synthesis or nucleic acid amplification (e.g., by the Polymerase Chain Reaction) or for any procedure utilizing reverse transcriptases in a variety of research, medical, diagnostic, forensic and agricultural applications.Type: ApplicationFiled: October 8, 2015Publication date: February 18, 2016Inventors: Ayoub Rashtchian, David Schuster
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Publication number: 20150050660Abstract: The present invention is directed to compositions comprising mixtures of reagents, including thermostable enzymes (e.g., thermostable DNA polymerases), buffers, cofactors and other components, suitable for immediate use in nucleic acid amplification or sequencing techniques without dilution or addition of further components. The compositions contain no stablizing agents (e.g., glycerol or serum albumin) and unexpectedly maintain activity for extended periods of time upon storage at temperatures above freezing. These compositions are useful, alone or in the form of kits, for nucleic acid amplification (e.g., by the Polymerase Chain Reaction) and sequencing (e.g., by dideoxy or “Sanger” sequencing), or for any procedure utilizing thermostable DNA polymerases in a variety of medical, forensic and agricultural applications. In particular, the compositions and methods are useful for amplifying and sequencing nucleic acid molecules that are larger than about 7 kilobases in size.Type: ApplicationFiled: August 22, 2014Publication date: February 19, 2015Inventors: Ayoub RASHTCHIAN, Joseph Solus
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Publication number: 20150037792Abstract: The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.Type: ApplicationFiled: July 11, 2014Publication date: February 5, 2015Inventors: Irina Nazarenko, Ayoub Rashtchian, Joseph Solus, Richard M. Pires, Marlene Darfler, Gulilat Gebeyehu, Mekbib Astatke
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Publication number: 20150017688Abstract: The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (RT-PCR). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step RT-PCR procedure using combinations of reverse transcriptase and thermostable DNA polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin. The invention thus facilitates the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of RNA molecules. The invention also is useful in the rapid production and amplification of cDNAs which may be used for a variety of industrial, medical and forensic purposes.Type: ApplicationFiled: September 26, 2014Publication date: January 15, 2015Inventors: Jun LEE, Ayoub RASHTCHIAN
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Patent number: 8741560Abstract: The present invention is directed to compositions comprising mixtures of reagents, including thermostable enzymes (e.g., thermostable DNA polymerases), buffers, cofactors and other components, suitable for immediate use in nucleic acid amplification or sequencing techniques without dilution or addition of further components. The compositions contain no stabilizing agents (e.g., glycerol or serum albumin) and unexpectedly maintain activity for extended periods of time upon storage at temperatures above freezing. These compositions are useful, alone or in the form of kits, for nucleic acid amplification (e.g., by the Polymerase Chain Reaction) and sequencing (e.g., by dideoxy or “Sanger” sequencing), or for any procedure utilizing thermostable DNA polymerases in a variety of medical, forensic and agricultural applications. In particular, the compositions and methods are useful for amplifying and sequencing nucleic acid molecules that are larger than about 7 kilobases in size.Type: GrantFiled: January 6, 2012Date of Patent: June 3, 2014Assignee: Life Technologies CorporationInventors: Ayoub Rashtchian, Joseph Solus
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Publication number: 20130252239Abstract: The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.Type: ApplicationFiled: January 28, 2013Publication date: September 26, 2013Applicant: Life Technologies CorporationInventors: Irina Nazarenko, Ayoub Rashtchian, Joseph Solus, Richard M. Pires, Marlene Darfler, Gulilat Gebeyehu, Mekbib Astatke
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Publication number: 20130244286Abstract: Methods for making cDNA molecules, for amplification of RNA by PCR and for preparation of cDNA libraries are provided. Kits for making cDNA molecules also are provided. Compositions are also provided comprising mixtures of reagents, including reverse transcriptases, buffers, cofactors and other components, suitable for immediate use in conversion of RNA into cDNA and RT PCR without dilution or addition of further components. These compositions are useful, alone or in the form of kits, for cDNA synthesis or nucleic acid amplification (e.g., by the Polymerase Chain Reaction) or for any procedure utilizing reverse transcriptases in a variety of research, medical, diagnostic, forensic and agricultural applications.Type: ApplicationFiled: March 4, 2013Publication date: September 19, 2013Applicant: QUANTA BIOSCIENCES, INC.Inventors: Ayoub Rashtchian, David Schuster
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Publication number: 20130149698Abstract: The present invention is directed to compositions comprising mixtures of reagents, including thermostable enzymes (e.g., thermostable DNA polymerases), buffers, cofactors and other components, suitable for immediate use in nucleic acid amplification or sequencing techniques without dilution or addition of further components. The compositions contain no stablizing agents (e.g., glycerol or serum albumin) and unexpectedly maintain activity for extended periods of time upon storage at temperatures above freezing. These compositions are useful, alone or in the form of kits, for nucleic acid amplification (e.g., by the Polymerase Chain Reaction) and sequencing (e.g., by dideoxy or “Sanger” sequencing), or for any procedure utilizing thermostable DNA polymerases in a variety of medical, forensic and agricultural applications. In particular, the compositions and methods are useful for amplifying and sequencing nucleic acid molecules that are larger than about 7 kilobases in size.Type: ApplicationFiled: January 6, 2012Publication date: June 13, 2013Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: AYOUB RASHTCHIAN, JOSEPH SOLUS
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Patent number: 8420324Abstract: Methods for making cDNA molecules, for amplification of RNA by PCR and for preparation of cDNA libraries are provided. Kits for making cDNA molecules also are provided. Compositions are also provided comprising mixtures of reagents, including reverse transcriptases, buffers, cofactors and other components, suitable for immediate use in conversion of RNA into cDNA and RT PCR without dilution or addition of further components. These compositions are useful, alone or in the form of kits, for cDNA synthesis or nucleic acid amplification (e.g., by the Polymerase Chain Reaction) or for any procedure utilizing reverse transcriptases in a variety of research, medical, diagnostic, forensic and agricultural applications.Type: GrantFiled: December 8, 2009Date of Patent: April 16, 2013Assignee: Quanta BiosciencesInventors: Ayoub Rashtchian, David Schuster
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Patent number: 8394609Abstract: The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.Type: GrantFiled: December 1, 2009Date of Patent: March 12, 2013Assignee: Life Technologies CorporationInventors: Irina Nazarenko, Ayoub Rashtchian, Joseph Solus, Richard Pires, Marlene Darfler, Gulilat Gebeyehu, Mekbib Astatke
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Patent number: 8372604Abstract: The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (RT-PCR). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step RT-PCR procedure using combinations of reverse transcriptase and thermostable DNA polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin. The invention thus facilitates the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of RNA molecules. The invention also is useful in the rapid production and amplification of cDNAs which may be used for a variety of industrial, medical and forensic purposes.Type: GrantFiled: December 29, 2010Date of Patent: February 12, 2013Assignee: Life Technologies CorporationInventors: Jun Lee, Ayoub Rashtchian
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Publication number: 20120142007Abstract: The present invention is directed to compositions comprising mixtures of reagents, including thermostable enzymes (e.g., thermostable DNA polymerases), buffers, cofactors and other components, suitable for immediate use in nucleic acid amplification or sequencing techniques without dilution or addition of further components. The compositions contain no stablizing agents (e.g., glycerol or serum albumin) and unexpectedly maintain activity for extended periods of time upon storage at temperatures above freezing. These compositions are useful, alone or in the form of kits, for nucleic acid amplification (e.g., by the Polymerase Chain Reaction) and sequencing (e.g., by dideoxy or “Sanger” sequencing), or for any procedure utilizing thermostable DNA polymerases in a variety of medical, forensic and agricultural applications. In particular, the compositions and methods are useful for amplifying and sequencing nucleic acid molecules that are larger than about 7 kilobases in size.Type: ApplicationFiled: January 6, 2012Publication date: June 7, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Ayoub Rashtchian, Joseph Solus
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Patent number: 8124340Abstract: Improved methods of studying RNA molecules are provided. In particular, methods of treating mixtures of RNA molecules so as to enrich the mixture for a desired type of RNA molecule are provided. For example, the methods permit depletion of mRNA from complex mixtures to facilitate study of microRNAs in the mixture.Type: GrantFiled: January 23, 2010Date of Patent: February 28, 2012Assignee: Quanta BiosciencesInventor: Ayoub Rashtchian