Patents by Inventor Azlinda B. Anwar

Azlinda B. Anwar has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Modified stem-loop oligonucleotide mediated reverse transcription and base-spacing constrained quantitative PCR
    Patent number: 9850527
    Abstract: There is provided a method for detecting a target RNA molecule in a sample comprising reverse transcription, amplification of the reverse transcription product, and detection of the amplification product, involving the use of (i) an RT oligonucleotide comprising a stem-loop portion containing one or more nucleotides modified or modifiable to block DNA polymerase extension and a target annealing portion that is complementary to a downstream portion of the target RNA, the target annealing portion located 3? to the stem-loop portion, (ii) a first amplification primer that anneals to a downstream portion of a 3? extended region of the reverse transcription product and (ii) a second amplification primer that anneals to an interface portion of a DNA strand complementary to the reverse transcription product, the interface portion comprising a region that is complementary to a 3? portion of the RT oligonucleotide and a 5? portion of the 3? extended region in the reverse transcription product.
    Type: Grant
    Filed: June 13, 2011
    Date of Patent: December 26, 2017
    Assignee: National University of Singapore
    Inventors: Heng Phon Too, Azlinda B. Anwar
  • MODIFIED STEM-LOOP OLIGONUCLEOTIDE MEDIATED REVERSE TRANSCRIPTION AND BASE-SPACING CONSTRAINED QUANTITATIVE PCR
    Publication number: 20130177915
    Abstract: There is provided a method for detecting a target RNA molecule in a sample.
    Type: Application
    Filed: June 13, 2011
    Publication date: July 11, 2013
    Applicant: National University of Singapore
    Inventors: Heng Phon Too, Azlinda B. Anwar