Abstract: This invention relates to a quick method for preparation of photorcactive polymers and immobilization of biomolecules onto these polymers. Particularly, this invention relates to a microwave-mediated rapid and efficient method for the preparation of photoreactive polymer having at least one photoactivable functional group capable of forming a covalent bond with the biomolecule in a photochemical reaction. Any matrix either organic or inorganic having a nucleophilic group preferably, amino, hydroxyl or thiol group can be modified by the invented method. Immobilization of biomolecules onto the photoreactive polymers was carried out by UV radiation. The method has applications for inmmobilization of bio-molecules irrespective of their functional groups in the fields of molecular biology, proteomics, genomics, diagnostics, chemical or biochemical industry and other related fields.