Patents by Inventor B. John Bergot

B. John Bergot has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Chromatographic separation of phosphorothioate oligonucleotides
    Patent number: 5395928
    Abstract: A method is provided for the preparation and/or analysis of synthetic phosphorothioate and -dithioate oligonucleotides. In particular, the method permits separation of fully sulfurized phosphorothioate or -dithioate oligonucleotides from incompletely sulfurized defect species on an anion exchange HPLC columns using concentration gradients of novel "soft base" anionic eluents, such as bromide, thiocyanate, and the like.
    Type: Grant
    Filed: May 10, 1993
    Date of Patent: March 7, 1995
    Assignee: Lynx Therapeutics, Inc.
    Inventor: B. John Bergot
  • Spectrally resolvable rhodamine dyes for nucleic acid sequence determination
    Patent number: 5366860
    Abstract: A spectrally resolvable set of rhodamine dyes are provided for use in the chain termination method of nucleic acid sequencing. A different rhodamine dye from the group consisting of tetramethylrhodamine, rhodamine X, rhodamine 6G, and rhodamine 110 is attached to the base of each of the dideoxynucleotides used in the sequencing method by way of an alkynylamino linker. Preferably, the labeled dideoxynucleotides are incorporated into the growing DNA chains by Taq DNA polymerase.
    Type: Grant
    Filed: September 29, 1989
    Date of Patent: November 22, 1994
    Assignee: Applied Biosystems, Inc.
    Inventors: B. John Bergot, Vergine Chakerian, Charles R. Connell, J. Scott Eadie, Steven Fung, N. Davis Hershey, Linda G. Lee, Steven M. Menchen, Sam L. Woo
  • Method of synthesizing sulfurized oligonucleotide analogs
    Patent number: 5292875
    Abstract: A method for synthesizing sulfurized oligonucleotide analogs, such as phosphorothioate and phosphorodithioate analogs, is provided that employs a thiophosphorus compound, such as a thiophosphoric, dithiophosphoric, thiophosphinic, or dithiophosphinic acid disulfide or polysulfide, as a sulfurizing agent. The method of the invention may be used to sulfurize any phosphorous(III)containing intermediate. Preferably, the method is practiced on a commercial DNA synthesizer using phosophoramidite and/or phosphorthioamidite intermediates.
    Type: Grant
    Filed: August 27, 1993
    Date of Patent: March 8, 1994
    Assignee: Lynx Therapeutics, Inc.
    Inventors: Wojciech J. Stec, Bogdan Uznanski, B. John Bergot, Bernard L. Hirschbein, Karen L. Fearson
  • Method for chromatographic separation of synthetic phosphorothioate oligonucleotides
    Patent number: 5183885
    Abstract: A method is provided for the preparation and/or analysis or synthetic phosphorothioate and -dithioate oligonucleotides. In particular, the method permits separation of fully sulfurized phosphorothioate or -dithioate oligonucleotides from incompletely sulfurized defect species on strong-anion exchange HPLC columns using concentration gradients of novel "soft base" anionic eluents, such as bromide, thiocyanate, and the like.
    Type: Grant
    Filed: October 18, 1991
    Date of Patent: February 2, 1993
    Assignee: Applied Biosystems, Inc.
    Inventor: B. John Bergot
  • Method of synethesizing sulfurized oligonucleotide analogs
    Patent number: 5151510
    Abstract: A method for synthesizing sulfurized oligonucleotide analogs, such as phosphorothioate and phosphorodithioate analogs, is provided that employs a thiophosphorus compound, such as a thiophosphoric, dithiophosphoric, thiophosphinic, or dithiophosphinic acid disulfide or polysulfide, as a sulfurizing agent. The method of the invention may be used to sulfurize any phosphorous(III)-containing intermediate. Preferably, the method is practiced on a commercial DNA synthesizer using phosphoramidite and/or phosphorthioamidite intermediates.
    Type: Grant
    Filed: April 20, 1990
    Date of Patent: September 29, 1992
    Assignee: Applied Biosystems, Inc.
    Inventors: Wojciech J. Stec, Bogdan Uznanski, B. John Bergot, Bernard L. Hirschbein, Karen L. Fearon