Abstract: The present disclosure provides novel compositions and methods for the production and use of polynucleotide sequences encoding a chimeric non-ribosomal peptide synthetase (NRPS) fusion protein for the biosynthesis of N-acylglycine biosurfactants within a heterologous expression system.

Abstract: The present disclosure provides novel compositions and methods for the production and use of polynucleotide sequences encoding a glycine N-acyltransferase protein (GLYAT, GLYATL 1, GLYATL 2, and GLYATL 3) for the biosynthesis of N-acylglycine biosurfactants within a heterologous expression system.

Abstract: A &bgr;-alanine NMTase was isolated from Lumonium latifolium. The purified enzyme catalyzes the N-methylation of &bgr;-alanine betaine, has an isoelectric point of about 5.15 and an apparent molecular weight of about 43 kilodaltons. A full-length cDNA encoding the NMTase was cloned by screening a L. latifolium cDNA library and was sequenced. Expression of the cDNA in transgenic, non-L. latifolium plants resulted in modulation of resistance to stress in the plants.