Abstract: A fluid impermeable transducer includes an assembly of a transducer head and a casing, and an actuator disposed in the casing rearward of the back of the transducer head and operable to transmit acoustic energy through the transducer head. The transducer head and casing define a working portion of the transducer that is fluid impermeable.

Abstract: Systems and methods described herein employ focused acoustic energy applied to a reservoir containing a fluid to eject a fluid sample from the fluid sample reservoir, e.g. to an inlet of an analytical device. In many embodiments, the ejected fluid sample traverses an air gap separating the inlet of the analytical device from an upper surface of the fluid in the fluid sample reservoir. In many embodiments, the ejected fluid sample comprises one or more droplets ejected from the fluid sample reservoir, which can contain particles suspended in the fluid sample.

Abstract: A method is provided for achieving transfection of host cells using sonoporation. An acoustic radiation generator is positioned in acoustic coupling relationship with respect to a reservoir containing host cells to be transfected, exogenous material to be incorporated into the host cells, and a cell-compatible fluid medium. The acoustic radiation generator is activated to generate acoustic radiation and direct the acoustic radiation into the reservoir in a manner effective to enable transfection of the host cells with the exogenous material.

Abstract: A method is provided for achieving transfection of host cells using sonoporation. An acoustic radiation generator is positioned in acoustic coupling relationship with respect to a reservoir containing host cells to be transfected, exogenous material to be incorporated into the host cells, and a cell-compatible fluid medium. The acoustic radiation generator is activated to generate acoustic radiation and direct the acoustic radiation into the reservoir in a manner effective to enable transfection of the host cells with the exogenous material.

Abstract: A method is provided for achieving transfection of host cells using sonoporation. An acoustic radiation generator is positioned in acoustic coupling relationship with respect to a reservoir containing host cells to be transfected, exogenous material to be incorporated into the host cells, and a cell-compatible fluid medium. The acoustic radiation generator is activated to generate acoustic radiation and direct the acoustic radiation into the reservoir in a manner effective to enable transfection of the host cells with the exogenous material.

Abstract: A method is provided for achieving transfection of host cells using sonoporation. An acoustic radiation generator is positioned in acoustic coupling relationship with respect to a reservoir containing host cells to be transfected, exogenous material to be incorporated into the host cells, and a cell-compatible fluid medium. The acoustic radiation generator is activated to generate acoustic radiation and direct the acoustic radiation into the reservoir in a manner effective to enable transfection of the host cells with the exogenous material.

Abstract: A fluid impermeable transducer includes an assembly of a transducer head and a casing, and an actuator disposed in the casing rearward of the back of the transducer head and operable to transmit acoustic energy through the transducer head. The transducer head and casing define a working portion of the transducer that is fluid impermeable.

Abstract: A method is provided for achieving transfection of host cells using sonoporation. An acoustic radiation generator is positioned in acoustic coupling relationship with respect to a reservoir containing host cells to be transfected, exogenous material to be incorporated into the host cells, and a cell-compatible fluid medium. The acoustic radiation generator is activated to generate acoustic radiation and direct the acoustic radiation into the reservoir in a manner effective to enable transfection of the host cells with the exogenous material.

Abstract: Apparatus and methods to form high efficiency and uniform Fresnel lens arrays for ultrasonic liquid manipulation are provided. An ultrasonic transducer array may be fabricated by forming top and bottom electrodes on top and bottom surfaces of a sensor plate. The ultrasonic transducer array may generate ultrasonic energy to manipulate one or more samples. Each of the top and bottom electrodes may be coupled to a radio frequency source and arranged to form one of a solid shape or a pattern. Additional apparatus and methods are disclosed.

Abstract: Apparatus and methods for ultrasonic coupling using ultrasonic radiation pressure generating are provided. Ultrasonic energy is generated to propagate in the form of ultrasonic waves in a medium coupled to a second element. The ultrasonic energy is converged in the medium to couple the medium to an object located at a distance from the second element. Additional apparatus and methods are disclosed.

Abstract: Apparatus and methods for ultrasonic coupling using ultrasonic radiation pressure generating are provided. Ultrasonic energy is generated to propagate in the form of ultrasonic waves in a medium coupled to a second element. The ultrasonic energy is converged in the medium to couple the medium to an object located at a distance from the second element. Additional apparatus and methods are disclosed.

Abstract: Apparatus and methods to form high efficiency and uniform Fresnel lens arrays for ultrasonic liquid manipulation are provided. An ultrasonic transducer array may be fabricated by forming top and bottom electrodes on top and bottom surfaces of a sensor plate. The ultrasonic transducer array may generate ultrasonic energy to manipulate one or more samples. Each of the top and bottom electrodes may be coupled to a radio frequency source and arranged to form one of a solid shape or a pattern. Additional apparatus and methods are disclosed.

Abstract: Various fluidic techniques can employ ducting structures, such as microstructures, that extend between other components, such as plate-like structures. A ducting structure can, for example, include an inlet opening toward or near one plate-like structure, an outlet opening toward or near another plate-like structure, and a duct in which fluid flows after being received through the inlet opening and before being provided through the outlet opening. In some implementations, a ducting structure is photo-defined, such as by exposing a photoimageable structure and then removing either exposed or unexposed regions. In some implementations, a ducting structure is a freestanding polymer microstructure. In some implementations, ducting structures are microstructures that extend approximately the same length between first and second plate-like structures, and have a ratio of length to maximum cavity diameter of approximately two or more.

Abstract: In accordance with one aspect of the present exemplary embodiment, provided is a hand-held printer system and method for printing on a target. The system includes a hand-held printer, a target position sensing system which senses a position of the target, and a hand-held printer position sensing system which senses a position of the hand-held printer relative to a printing surface of the target. A control mechanism actuates the printing of the hand-held printer based on the sensed positions.

Abstract: The present invention provides a method and device for the acoustic ejection of fluid droplets having a constant drop size from fluid-containing reservoirs having varying fluid heights contained therein without the need for repositioning the acoustic ejector in the z direction by adjusting the RF frequency and amplitude. In one embodiment, the device is comprised of: a plurality of reservoirs each adapted to contain a fluid; an ejector comprising a means for generating acoustic radiation, means for controlling the RF frequency and amplitude used to generate the acoustic radiation, means for focusing the acoustic radiation at a focal point near the fluid surface in each of the reservoirs; and a means for positioning the ejector in acoustically coupled relationship to each of the reservoirs. The invention is useful in a number of contexts, particularly in the preparation of biomolecular arrays.

Abstract: The present invention provides a method and device for the acoustic ejection of fluid droplets having a constant drop size from fluid-containing reservoirs having varying fluid heights contained therein without the need for repositioning the acoustic ejector in the z direction by adjusting the RF frequency and amplitude. In one embodiment, the device is comprised of: a plurality of reservoirs each adapted to contain a fluid; an ejector comprising a means for generating acoustic radiation, means for controlling the RF frequency and amplitude used to generate the acoustic radiation, means for focusing the acoustic radiation at a focal point near the fluid surface in each of the reservoirs; and a means for positioning the ejector in acoustically coupled relationship to each of the reservoirs. The invention is useful in a number of contexts, particularly in the preparation of biomolecular arrays.

Abstract: An active controller is provided to control operation of an acoustic printhead in which impedance of the printhead changes with both the active printing row and the distribution of active ejectors in the row. The active controller varies the input power to the printhead in response to active ejectors. Power requirements are insured while also reducing power consumption and maintaining print quality. The determination of RF power is based on the active row, how many ink ejectors are to be switched on, and their position within a row. Look-up tables used to determine proper attenuation or power values are provided in a cascaded fashion.

Abstract: An apparatus and method for ink-jet printing on a recording medium is provided which includes the steps of jetting aqueous ink drops on paper in the form of an image. The aqueous ink used is a slow-drying (high-surface tension) ink which does not penetrate the paper/paper fibers for a relatively long time. Prior to penetration of the paper/paper fibers, the water in the droplet is quickly evaporated from the ink while still resident on the paper surface. The evaporation process is substantially completed prior to an additional liquid ink being jetted onto the same or adjoining location of the recording medium. The evaporation is rapid enough to prevent the resident ink from substantially migrating/wicking to any adjacent location or into the recording medium. Further the drying energy is transferred to the resident ink spots from the same direction as the printheads ensuring less energy requirement.

Abstract: An apparatus for ink-jet printing on a recording medium is provided which includes the steps of jetting aqueous ink drops on paper in the form of an image. The aqueous ink used is a slow-drying (high-surface tension) ink which does not penetrate the paper/paper fibers for a relatively long time. Prior to penetration of the paper/paper fibers, the water in the droplet is quickly evaporated from the ink while still resident on the paper surface. The evaporation process is substantially completed prior to an additional liquid ink being jetted onto the same or adjoining location of the recording medium. The evaporation is rapid enough to prevent the resident ink from substantially migrating/wicking to any adjacent location or into the recording medium. Further the drying energy is transferred to the resident ink spots from the same direction as the printheads ensuring less energy requirement.

Abstract: An apparatus for ink-jet printing on a recording medium is provided which includes the steps of jetting aqueous ink drops on paper in the form of an image. The aqueous ink used is a slow-drying (high-surface tension) ink which does not penetrate the paper/paper fibers for a relatively long time. Prior to penetration of the paper/paper fibers, the water in the droplet is quickly evaporated from the ink while still resident on the paper surface. The evaporation process is substantially completed prior to an additional liquid ink being jetted onto the same or adjoining location of the recording medium. The evaporation is rapid enough to prevent the resident ink from substantially migrating/wicking to any adjacent location or into the recording medium. Further the drying energy is transferred to the resident ink spots from the same direction as the printheads ensuring less energy requirement.