Patents by Inventor Baek Joong KIM

Baek Joong KIM has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20250197872
    Abstract: An exemplary embodiment of the present disclosure provides an allulose epimerase expression cassette including a polynucleotide encoding allulose epimerase and a promoter which is operably linked thereto and regulates the expression of the allulose epimerase in a Corynebacterium genus strain. The promoter consists of a base sequence represented by SEQ ID NO: 1, a base sequence represented by SEQ ID NO: 9, a base sequence represented by SEQ ID NO: 10, or a base sequence represented by SEQ ID NO: 11. The allulose epimerase expression cassette may be hardly operated under a normal environment of culturing a recombinant Corynebacterium genus strain and does not induce growth inhibition of the recombinant Corynebacterium genus strain. The recombinant Corynebacterium genus strain transformed with the allulose epimerase expression cassette may grow and proliferate normally until a logarithmic phase and stably and highly express allulose epimerase after the logarithmic phase.
    Type: Application
    Filed: August 19, 2024
    Publication date: June 19, 2025
    Applicant: DAESANG CORPORATION
    Inventors: Baek Joong KIM, Eun Seok CHOI, Tae Gyun KIM
  • Publication number: 20250197871
    Abstract: One embodiment of the present disclosure provides a promoter consisting of a base sequence represented by SEQ ID NO: 6 or a base sequence represented by SEQ ID NO: 27 and regulating the expression of allulose epimerase in a Corynebacterium sp. strain. According to the present disclosure, the novel promoter may constitutively and highly express a target protein, particularly an enzyme, in a Corynebacterium sp. strain. For example, by using a recombinant Corynebacterium sp. strain transformed with an expression vector including the novel promoter according to the present disclosure, it is possible to economically mass-produce allulose epimerase or economically mass-produce allulose from fructose.
    Type: Application
    Filed: July 15, 2024
    Publication date: June 19, 2025
    Applicant: DAESANG CORPORATION
    Inventors: Eun Seok CHOI, Baek Joong KIM
  • Publication number: 20250075237
    Abstract: The present invention provides a novel promoter variant in which some nucleotides of the glyceraldehyde-3-phosphate dehydrogenase (gapA) gene promoter of Escherichia coli are deleted. The novel promoter variant according to the present invention can constitutively express a target protein at a high level, particularly an enzyme, in Escherichia coli. Therefore, a target protein, particularly an enzyme, can be economically mass-produced using a recombinant strain transformed with an expression vector containing the novel promoter variant according to the present invention. For example, allulose epimerase can be economically mass-produced or allulose can be economically mass-produced from fructose using a recombinant strain transformed with an expression vector containing the novel promoter variant according to the present invention.
    Type: Application
    Filed: December 29, 2021
    Publication date: March 6, 2025
    Applicant: DAESANG CORPORATION
    Inventors: Ji Ha LEE, Eun Seok CHOI, Baek Joong KIM, Tae Gyun KIM, Da Han JUNG, Seung Woo CHO, Hak Jun KIM
  • Patent number: 12221639
    Abstract: The present invention provides a novel allulose epimerase variant in which an amino acid residue present at a specific position of an amino acid sequence of wild-type D-allulose 3-epimerase derived from Flavonifractor plautii is substituted with another amino acid residue, and various uses thereof. The novel allulose epimerase variant according to the present invention has a higher conversion activity of fructose to allulose than that of the wild-type D-allulose 3-epimerase derived from Flavonifractor plautii or a D-allulose epimerase variant W29K/G216S/M234I, and has excellent thermal stability especially under high temperature conditions of 60° C. or higher, thereby preventing contamination during an industrial-scale enzymatic conversion reaction for the mass production of allulose, shortening the production time, and reducing the production costs.
    Type: Grant
    Filed: November 22, 2021
    Date of Patent: February 11, 2025
    Assignee: DAESANG CORPORATION
    Inventors: Ji Ha Lee, Tae Gyun Kim, Baek Joong Kim, Da Han Jung, Seung Woo Cho, Hak Jun Kim
  • Publication number: 20240052389
    Abstract: The present invention provides a novel allulose epimerase variant in which an amino acid residue present at a specific position of an amino acid sequence of wild-type D-allulose 3-epimerase derived from Flavonifractor plautii is substituted with another amino acid residue, and various uses thereof. The novel allulose epimerase variant according to the present invention has a higher conversion activity of fructose to allulose than that of the wild-type D-allulose 3-epimerase derived from Flavonifractor plautii or a D-allulose epimerase variant W29K/G216S/M234I, and has excellent thermal stability especially under high temperature conditions of 60° C. or higher, thereby preventing contamination during an industrial-scale enzymatic conversion reaction for the mass production of allulose, shortening the production time, and reducing the production costs.
    Type: Application
    Filed: November 22, 2021
    Publication date: February 15, 2024
    Applicant: DAESANG CORPORATION
    Inventors: Ji Ha LEE, Tae Gyun KIM, Baek Joong KIM, Da Han JUNG, Seung Woo CHO, Hak Jun KIM
  • Patent number: 11447766
    Abstract: An embodiment of the present disclosure provides an apparatus for immobilizing a microbial cell, the apparatus including: a mixing tank in which a cell-carrier-containing mixed solution is accommodated; a nozzle part through which the cell-carrier-containing mixed solution is injected from the mixing tank and is discharged to the outside; and a reaction tank in which a cell immobilized bead is formed by contact between the cell-carrier-containing mixed solution discharged from the nozzle part and an aqueous curing agent solution. In the apparatus for immobilizing a microbial cell according to the present disclosure, since the cell-carrier-containing mixed solution is injected through an air spraying nozzle, even when an immobilized carrier solution having a high viscosity is used, a microbial cell immobilized bead having a small size and having a spherical shape, or an almost spherical shape may be mass-produced.
    Type: Grant
    Filed: December 21, 2016
    Date of Patent: September 20, 2022
    Assignee: DAESANG CORPORATION
    Inventors: Baek Joong Kim, Tae Gun Kim, Min Su Kim, Tae Yong Kim, Eun Bum Song
  • Publication number: 20190002866
    Abstract: An embodiment of the present disclosure provides an apparatus for immobilizing a microbial cell, the apparatus including: a mixing tank in which a cell-carrier-containing mixed solution is accommodated; a nozzle part through which the cell-carrier-containing mixed solution is injected from the mixing tank and is discharged to the outside; and a reaction tank in which a cell immobilized bead is formed by contact between the cell-carrier-containing mixed solution discharged from the nozzle part and an aqueous curing agent solution. In the apparatus for immobilizing a microbial cell according to the present disclosure, since the cell-carrier-containing mixed solution is injected through an air spraying nozzle, even when an immobilized carrier solution having a high viscosity is used, a microbial cell immobilized bead having a small size and having a spherical shape, or an almost spherical shape may be mass-produced.
    Type: Application
    Filed: December 21, 2016
    Publication date: January 3, 2019
    Inventors: Baek Joong KIM, Tae Gun KIM, Min Su KIM, Tae Yong KIM, Eun Bum SONG