Abstract: The present disclosure provides compositions and methods for producing hydrogel matrix constructs. Methods of using hydrogel matrix constructs for tissue repair and regeneration and for the oxygenation of red blood cells are also disclosed.

Abstract: Some embodiments of the disclosure disclose manifolds, microfluidic systems and methods that provide control over fluid flow distribution to an array of bio-scaffolds contained within the manifolds. In some embodiments, multiple perfusates may be injected into the manifold via multiple inlets where the manifold contains a bio-assembly with a substrate having a bio-scaffold disposed thereon. Biological investigations of the perfusates may then be conducted in the vascular components and chambers of the bio-scaffold.

Abstract: A method of preparing a structure is provided. The method includes providing an initial structure; casting a first material in one or more void volumes of the initial structure; removing the initial structure from the first material; obtaining a cast structure comprising the first material; coating a second material on the cast structure; casting a third material using the coated cast structure; removing the first material; and obtaining a final structure. In various embodiments, the initial structure can include a first initial structure and a second initial structure and casting a first material in one or more first void volumes of the first initial structure and in one or more second void volumes of the second initial structure. In various embodiments, the method includes assembling the first cast structure and the second cast structure and obtaining an assembled structure comprising the first cast structure and the second cast structure.

Abstract: Provided herein is a system for producing a product. The system generally comprises a large-area micro-stereolithography system and a layer leveling system. The large-area micro-stereolithography system is capable of generating the product by optically polymerizing successive layers of a curable resin at a print plane. The layer leveling system is capable of flattening a non-flat region (such as a meniscus) of the curable resin in a vicinity of the build plane.

Abstract: Some embodiments of the disclosure disclose a process for adhering cells, beads or particles to a surface of a material. The surface may be flat or curved and can be employed in the context of patterned or 3D printed hollow channels, facilitating the recapitulation of certain aspects of physiological systems. In various embodiments, interfacial cell seeding is accomplished by locally polymerizing a carrier containing a suspension of cells along the surface with a crosslinking molecule incorporated into the target material in advance of the interfacial polymerization. Polymerization of the carrier entraps the cells into controlled configuration along the surface. The techniques disclosed herein can be utilized for tissue engineering to build engineered organs/devices suitable for implanting into living organisms.

Abstract: The present disclosure provides compositions and methods for producing hydrogel matrix constructs. Methods of using hydrogel matrix constructs for tissue repair and regeneration and for the oxygenation of red blood cells are also disclosed.

Abstract: The present disclosure provides compositions and methods for producing hydrogel matrix constructs. Methods of using hydrogel matrix constructs for tissue repair and regeneration and for the oxygenation of red blood cells are also disclosed.

Abstract: Various precursor solutions and methods of 3D printing and other additive manufacturing approaches are provided for the manufacture of articles using photocrosslinkable vinyl shape memory polymers. In various aspects, articles are manufactured by a process comprising (i) exposing a precursor solution to an intensity and frequency of light to initiate photo-polymerization of the precursor solution to form a layer of the article; and (ii) repeating step (i) a number of times to form the article in a layer-by-layer approach; wherein the precursor solution comprises a first polymeric precursor comprising a plurality of vinyl terminated side-chains attached thereto. A vinyl-functionalized, photocrosslinkable SMP used as an example herein is a novel variant of a previously disclosed SMP composition, with higher amounts of vinyl functionalization but similar thermomechanical properties to the SMP library previously disclosed.

Abstract: The present disclosure provides compositions and methods for producing hydrogel matrix constructs. Methods of using hydrogel matrix constructs for tissue repair and regeneration and for the oxygenation of red blood cells are also disclosed.

Abstract: The disclosure describes new devices and methods for vascularizing devices and methods for implanted diagnostics and therapeutics. The present disclosure provides, in certain embodiments, a device containing a degradable shell and a non-degradable core. In certain embodiments, the non-degradable core can include encapsulated therapeutic cells and/or biosensors, wherein the degradable shell serves as a scaffold for blood vessel growth, resulting in enhanced blood flow to the cells and/or biosensors.

Abstract: A bio-assembly, a kit for a bio-assembly and methods of using the same are provided. The bio-assembly includes a substrate and a bio-scaffold affixed to the substrate. In accordance with various embodiments, a loader plate having a partition or a loader and a plate having a partition are provided. In accordance with various embodiments, the loader plate includes a partition outlet and a partition inlet, wherein the partition outlet and the partition inlet in fluid communication with the gel. In accordance with various embodiments, the partition comprising an internal volume and shaped to receive the bio-scaffold, and the loader includes a loader inlet and a loader outlet in fluid communication with the gel. In accordance with various embodiments, a bio-compatible adhesive positioned between the substrate and the loader plate or plate. In accordance with various embodiments, a fluid mixture is injected into the bio-scaffold.

Abstract: The disclosure provides biocompatible heat exchangers, artificial tissues, systems, and thermofluidic methods for spatiotemporal control of biological signaling and gene expression. The disclosure demonstrates that in heat exchangers containing embedded cells with heat-activatable transgenes, gene expression patterning can be tuned both spatially and dynamically by varying channel network architecture, fluid temperature, fluid flow direction, and stimulation timing in a user-defined manner and maintained in vivo.

Abstract: The present disclosure provides compositions and methods for producing hydrogel matrix constructs. Methods of using hydrogel matrix constructs for tissue repair and regeneration and for the oxygenation of red blood cells are also disclosed.

Abstract: The present disclosure provides compositions and methods for producing hydrogel matrix constructs. Methods of using hydrogel matrix constructs for tissue repair and regeneration and for the oxygenation of red blood cells are also disclosed.

Abstract: The present disclosure provides compositions and methods for producing hydrogel matrix constructs. Methods of using hydrogel matrix constructs for tissue repair and regeneration and for the oxygenation of red blood cells are also disclosed.

Abstract: A device made from a hydrogel matrix is provided. The hydrogel matrix includes a photoabsorber with a void architecture in the matrix, having a first vessel architecture and a second vessel architecture that are each tubular and branching, wherein the first and second vessel architectures are fluidically independent from each other. A pre-polymerization solution for forming the device, and methods of fabricating such devices are described. A method of fabricating a 3D hydrogel construct is provided. The method includes using a computer-implemented process to create a 3D model of the construct based on a tessellation of polyhedra having a number of faces connected by edges and vertices, generate a first vascular component of the model, generate a second vascular component of the model, and combine the first and second vascular components of the model.

Abstract: The present disclosure provides compositions and methods for producing hydrogel matrix constructs. Methods of using hydrogel matrix constructs for tissue repair and regeneration and for the oxygenation of red blood cells are also disclosed.