Patents by Inventor Bakthan Singaram

Bakthan Singaram has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10488418
    Abstract: Provided is a method for quantifying sugar appearing in a biological fluid of a subject, the method include (a) obtaining a biological sample from a subject, who has ingested one or more sugars, over a period of 0 to 24 hours after the ingesting; and (b) measuring the amount of the one or more sugars in the biological sample with an organoborane compound coupled to a fluorophore. Also provided are assays and kits for performing the above methods.
    Type: Grant
    Filed: September 2, 2015
    Date of Patent: November 26, 2019
    Assignee: The Regents of the University of California
    Inventors: Bakthan Singaram, Angel Resendez, Dominic Luc Webb
  • Patent number: 10274483
    Abstract: Disclosed are systems for measuring the concentration of a disaccharide in an aqueous medium, the system comprising a boronic acid derivative and a fluorescent molecule. Also disclosed are methods of determining gastrointestinal permeability in a subject, the method comprising contacting an aqueous medium obtained from a subject with the above system to obtain a mixture, where the patient has consumed a known quantity of a disaccharide; measuring the fluorescence emission of the mixture; and correlating the extent of fluorescence emission to the concentration of the disaccharide in the aqueous medium; and correlating the concentration of the disaccharide in the aqueous medium to the gastrointestinal permeability in the subject.
    Type: Grant
    Filed: June 5, 2015
    Date of Patent: April 30, 2019
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFONIA
    Inventors: Bakthan Singaram, Angel Resendez, Dominic-Luc Webb
  • Publication number: 20170285038
    Abstract: The present disclosure relates to a method for quantifying sugar appearing in a biological fluid of a subject, the method comprising: (a) obtaining a biological sample from a subject, who has ingested one or more sugars, over a period of 0 to 24 hours after the ingesting; and (b) measuring the amount of the one or more sugars in the biological sample with an organoborane compound coupled to a fluorophore. Disclosed are also assays and kits for performing the above methods.
    Type: Application
    Filed: September 2, 2015
    Publication date: October 5, 2017
    Inventors: Bakthan SINGARAM, Angel RESENDEZ, Dominic Luc WEBB
  • Publication number: 20170184574
    Abstract: Disclosed are systems for measuring the concentration of a disaccharide in an aqueous medium, the system comprising a boronic acid derivative and a fluorescent molecule. Also disclosed are methods of determining gastrointestinal permeability in a subject, the method comprising contacting an aqueous medium obtained from a subject with the above system to obtain a mixture, where the patient has consumed a known quantity of a disaccharide; measuring the fluorescence emission of the mixture; and correlating the extent of fluorescence emission to the concentration of the disaccharide in the aqueous medium; and correlating the concentration of the disaccharide in the aqueous medium to the gastrointestinal permeability in the subject.
    Type: Application
    Filed: June 5, 2015
    Publication date: June 29, 2017
    Inventors: Bakthan SINGARAM, Angel RESENDEZ, Dominic-Luc WEBB
  • Patent number: 9243004
    Abstract: Boronic esters and boronic acids are synthesized at ambient temperature in an ethereal solvent by the reaction of Grignard reagents with a boron-containing substrate. The boron-containing substrate may be a boronic ester such as pinacolborane, neopentylglycolborane, or a dialkylaminoborane compound such as diisopropylaminoborane. The Grignard reagents may be pre-formed or generated from an alkyl, alkenyl, aryl, arylalkyl, heteroaryl, vinyl, or allyl halide compound and Mg0. When the boron-containing substrate is a boronic ester, the reactions generally proceed at room temperature without added base in about 1 to 3 hours to form a boronic ester compound. When the boron-containing substrate is a dialkylaminoborane compound, the reactions generally proceed to completion at 0° C. in about 1 hour to form a boronic acid compound.
    Type: Grant
    Filed: July 20, 2012
    Date of Patent: January 26, 2016
    Assignee: The Regents of the University of California
    Inventors: Jacob W. Clary, Bakthan Singaram
  • Publication number: 20150119580
    Abstract: Boronic esters and boronic acids are synthesized at ambient temperature in an ethereal solvent by the reaction of Grignard reagents with a boron-containing substrate. The boron-containing substrate may be a boronic ester such as pinacolborane, neopentylglycolborane, or a dialkylaminoborane compound such as diisopropylaminoborane. The Grignard reagents may be pre-formed or generated from an alkyl, alkenyl, aryl, arylalkyl, heteroaryl, vinyl, or allyl halide compound and Mg0. When the boron-containing substrate is a boronic ester, the reactions generally proceed at room temperature without added base in about 1 to 3 hours to form a boronic ester compound. When the boron-containing substrate is a dialkylaminoborane compound, the reactions generally proceed to completion at 0° C. in about 1 hour to form a boronic acid compound.
    Type: Application
    Filed: July 20, 2012
    Publication date: April 30, 2015
    Applicant: The Regents of the University of California
    Inventors: Jacob W. Clary, Bakthan Singaram
  • Patent number: 8980073
    Abstract: Disclosed are methods and devices for detection of ion migration and binding, utilizing a nanopipette adapted for use in an electrochemical sensing circuit. The nanopipette may be functionalized on its interior bore with metal chelators for binding and sensing metal ions or other specific binding molecules such as boronic acid for binding and sensing glucose. Such a functionalized nanopipette is comprised in an electrical sensor that detects when the nanopipette selectively and reversibly binds ions or small molecules. Also disclosed is a nanoreactor, comprising a nanopipette, for controlling precipitation in aqueous solutions by voltage-directed ion migration, wherein ions may be directed out of the interior bore by a repulsing charge in the bore.
    Type: Grant
    Filed: March 2, 2012
    Date of Patent: March 17, 2015
    Assignee: The Regents of the University of California
    Inventors: Nader Pourmand, Boaz Vilozny, Paolo Actis, R. Adam Seger, Bakthan Singaram
  • Patent number: 8951503
    Abstract: The present invention concerns an improved optical method and optical sensing device for determining the levels of polyhydroxyl-substituted organic molecules in vitro and/or in vivo in aqueous media. The range of detection is between about 400 and 800 nm. In particular, a sensory devise is implemented in a mammal to determine sugar levels. Specifically, a dye is combined with a conjugated nitrogen-containing heterocyclic aromatic boronic acid-substituted bis-onium compound in the presence of a sugar, such as fructose or glucose. The viologens are preferred as the aromatic conjugated nitrogen-containing boronic acid substituted compounds. The method is useful to determine sugar levels in a human being.
    Type: Grant
    Filed: August 8, 2008
    Date of Patent: February 10, 2015
    Assignee: The Regents of the University of California
    Inventors: Bakthan Singaram, Ritchie A. Wessling
  • Patent number: 8394357
    Abstract: The present invention concerns an improved optical method and optical sensing device for determining the levels of polyhydroxyl-substituted organic molecules in vitro and/or in vivo in aqueous media. The range of detection is between about 400 and 800 nm. In particular, a sensory devise is implemented in a mammal to determine sugar levels. Specifically, a dye is combined with a conjugated nitrogen-containing heterocyclic aromatic boronic acid-substituted bis-onium compound in the presence of a sugar, such as fructose or glucose. The viologens are preferred as the aromatic conjugated nitrogen-containing boronic acid substituted compounds. The method is useful to determine sugar levels in a human being.
    Type: Grant
    Filed: August 8, 2008
    Date of Patent: March 12, 2013
    Assignee: The Regents of the University of California
    Inventors: Bakthan Singaram, Ritchie A. Wessling
  • Publication number: 20120222958
    Abstract: Disclosed are methods and devices for detection of ion migration and binding, utilizing a nanopipette adapted for use in an electrochemical sensing circuit. The nanopipette may be functionalized on its interior bore with metal chelators for binding and sensing metal ions or other specific binding molecules such as boronic acid for binding and sensing glucose. Such a functionalized nanopipette is comprised in an electrical sensor that detects when the nanopipette selectively and reversibly binds ions or small molecules. Also disclosed is a nanoreactor, comprising a nanopipette, for controlling precipitation in aqueous solutions by voltage-directed ion migration, wherein ions may be directed out of the interior bore by a repulsing charge in the bore.
    Type: Application
    Filed: March 2, 2012
    Publication date: September 6, 2012
    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Nader Pourmand, Boaz Vilozny, Paolo Actis, R. Adam Seger, Bakthan Singaram
  • Publication number: 20120009126
    Abstract: The present invention concerns an improved optical method and optical sensing device for determining the levels of polyhydroxyl-substituted organic molecules in vitro and/or in vivo in aqueous media. The range of detection is between about 400 and 800 nm. In particular, a sensory devise is implemented in a mammal to determine sugar levels. Specifically, a dye is combined with a conjugated nitrogen-containing heterocyclic aromatic boronic acid-substituted bis-onium compound in the presence of a sugar, such as fructose or glucose. The viologens are preferred as the aromatic conjugated nitrogen-containing boronic acid substituted compounds. The method is useful to determine sugar levels in a human being.
    Type: Application
    Filed: June 9, 2011
    Publication date: January 12, 2012
    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Bakthan SINGARAM, Ritchie A. WESSLING
  • Patent number: 7968714
    Abstract: The present invention concerns an improved optical method and optical sensing device for determining the levels of polyhydroxyl-substituted organic molecules in vitro and/or in vivo in aqueous media. The range of detection is between about 400 and 800 nm. In particular, a sensory devise is implemented in a mammal to determine sugar levels. Specifically, a dye is combined with a conjugated nitrogen-containing heterocyclic aromatic boronic acid-substituted bis-onium compound in the presence of a sugar, such as fructose or glucose. The viologens are preferred as the aromatic conjugated nitrogen-containing boronic acid substituted compounds. The method is useful to determine sugar levels in a human being.
    Type: Grant
    Filed: August 8, 2008
    Date of Patent: June 28, 2011
    Assignee: Regents of the University of California
    Inventors: Bakthan Singaram, Ritchie A. Wessling
  • Publication number: 20090149656
    Abstract: The present invention concerns an improved optical method and optical sensing device for determining the levels of polyhydroxyl-substituted organic molecules in vitro and/or in vivo in aqueous media. The range of detection is between about 400 and 800 nm. In particular, a sensory devise is implemented in a mammal to determine sugar levels. Specifically, a dye is combined with a conjugated nitrogen-containing heterocyclic aromatic boronic acid-substituted bis-onium compound in the presence of a sugar, such as fructose or glucose. The viologens are preferred as the aromatic conjugated nitrogen-containing boronic acid substituted compounds. The method is useful to determine sugar levels in a human being.
    Type: Application
    Filed: August 8, 2008
    Publication date: June 11, 2009
    Inventors: Bakthan Singaram, Ritchie A. Wessling
  • Publication number: 20090148956
    Abstract: The present invention concerns an improved optical method and optical sensing device for determining the levels of polyhydroxyl-substituted organic molecules in vitro and/or in vivo in aqueous media. The range of detection is between about 400 and 800 nm. In particular, a sensory devise is implemented in a mammal to determine sugar levels. Specifically, a dye is combined with a conjugated nitrogen-containing heterocyclic aromatic boronic acid-substituted bis-onium compound in the presence of a sugar, such as fructose or glucose. The viologens are preferred as the aromatic conjugated nitrogen-containing boronic acid substituted compounds. The method is useful to determine sugar levels in a human being.
    Type: Application
    Filed: August 8, 2008
    Publication date: June 11, 2009
    Inventors: Bakthan Singaram, Ritchie A. Wessling
  • Publication number: 20090148344
    Abstract: The present invention concerns an improved optical method and optical sensing device for determining the levels of polyhydroxyl-substituted organic molecules in vitro and/or in vivo in aqueous media. The range of detection is between about 400 and 800 nm. In particular, a sensory devise is implemented in a mammal to determine sugar levels. Specifically, a dye is combined with a conjugated nitrogen-containing heterocyclic aromatic boronic acid-substituted bis-onium compound in the presence of a sugar, such as fructose or glucose. The viologens are preferred as the aromatic conjugated nitrogen-containing boronic acid substituted compounds. The method is useful to determine sugar levels in a human being.
    Type: Application
    Filed: August 8, 2008
    Publication date: June 11, 2009
    Inventors: Bakthan Singaram, Ritchie A. Wessling
  • Patent number: 7470420
    Abstract: The present invention concerns an improved optical method and optical sensing device for determining the levels of polyhydroxyl-substituted organic molecules in vitro and/or in vivo in aqueous media. The range of detection is between about 400 and 800 nm. In particular, a sensory devise is implemented in a mammal to determine sugar levels. Specifically, a dye is combined with a conjugated nitrogen-containing heterocyclic aromatic boronic acid-substituted bis-onium compound in the presence of a sugar, such as fructose or glucose. The viologens are preferred as the aromatic conjugated nitrogen-containing boronic acid substituted compounds. The method is useful to determine sugar levels in a human being.
    Type: Grant
    Filed: December 7, 2005
    Date of Patent: December 30, 2008
    Assignee: The Regents of the University of California
    Inventors: Bakthan Singaram, Ritchie A. Wessling
  • Patent number: 7060507
    Abstract: Targeted molecular bar codes and methods for using the same are provided. The subject targeted molecular bar codes include a molecular bar code and a member of a specific binding pair, where the specific binding pair member is generally bonded to the bar code through a linking group. The subject molecular bar code may be read during translocation through a single nano-meter scale pore. The subject targeted molecular bar codes find use in a variety of different applications involving analyte detection, such as screening and diagnostic applications.
    Type: Grant
    Filed: August 13, 2002
    Date of Patent: June 13, 2006
    Assignee: The Regents of the University of California
    Inventors: Mark Akeson, David W. Deamer, Wenonah Vercoutere, Hugh E. Olsen, Rebecca Braslau, Bakthan Singaram, Derek Steiner, Frank Cappuccio
  • Publication number: 20060083688
    Abstract: The present invention concerns an improved optical method and optical sensing device for determining the levels of polyhydroxyl-substituted organic molecules in vitro and/or in vivo in aqueous media. The range of detection is between about 400 and 800 nm. In particular, a sensory devise is implemented in a mammal to determine sugar levels. Specifically, a dye is combined with a conjugated nitrogen-containing heterocyclic aromatic boronic acid-substituted bis-onium compound in the presence of a sugar, such as fructose or glucose. The viologens are preferred as the aromatic conjugated nitrogen-containing boronic acid substituted compounds. The method is useful to determine sugar levels in a human being.
    Type: Application
    Filed: December 7, 2005
    Publication date: April 20, 2006
    Inventors: Bakthan Singaram, Ritchie Wessling
  • Publication number: 20060063196
    Abstract: Targeted molecular bar codes and methods for using the same are provided. The subject targeted molecular bar codes include a molecular bar code and a member of a specific binding pair, where the specific binding pair member is generally bonded to the bar code through a linking group. The subject molecular bar code may be read during translocation through a single nano-meter scale pore. The subject targeted molecular bar codes find use in a variety of different applications involving analyte detection, such as screening and diagnostic applications.
    Type: Application
    Filed: August 13, 2002
    Publication date: March 23, 2006
    Inventors: Mark Akeson, David Deamer, Wenonah Vercoutere, Hugh Olsen, Rebecca Braslau, Bakthan Singaram, Derek Steiner, Frank Cappuccio
  • Publication number: 20040028612
    Abstract: The present invention concerns an improved optical method and optical sensing device for determining the levels of polyhydroxyl-substituted organic molecules in vitro and/or in vivo in aqueous media. In particular, a sensory devise is implemented in a mammal to determine sugar levels. Specifically, a dye is combined with a conjugated nitrogen-containing heterocyclic aromatic boronic acid-substituted bis-onium compound in the presence of a sugar, such as fructose or glucose. The viologens are preferred as the aromatic conjugated nitrogen-containing boronic acid substituted compounds. The method is useful to determine sugar levels in a human being.
    Type: Application
    Filed: June 5, 2003
    Publication date: February 12, 2004
    Inventors: Bakthan Singaram, Ritchie A. Wessling