Patents by Inventor Balasubrahmanyam Addepalli

Balasubrahmanyam Addepalli has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • ENGINEERED PROTEASES WITH ENHANCED AUTOLYSIS RESISTANCE
    Publication number: 20240084279
    Abstract: This present disclosure relates to engineered protease enzymes, including trypsin, Lys-C and Asp-N proteases, that have enhanced autolysis resistance. Also disclosed herein are methods of using such engineered enzymes for improving detection of target analyte proteins in an analytical assay.
    Type: Application
    Filed: August 7, 2023
    Publication date: March 14, 2024
    Applicant: Waters Technologies Corporation
    Inventors: Balasubrahmanyam Addepalli, Abraham S. Finny, Matthew A. Lauber
  • Recombinant nucleoside-specific ribonuclease and method of producing and using same
    Patent number: 11535834
    Abstract: A recombinant ribonuclease is disclosed. The recombinant ribonuclease is produced by introducing a recombinant DNA sequence into a host; activating expression of the recombinant DNA sequence within the host to produce the recombinant ribonuclease; and isolating the recombinant ribonuclease from the host. Additionally, a method of analyzing an RNA sequence includes digesting the RNA with a first recombinant ribonuclease to give digestion products comprising nucleotides of the RNA sequence; and analyzing the digestion products using an analytical method to provide the identity of at least some of the nucleotides. The recombinant ribonuclease includes at least one of a uridine-specific recombinant RNase MC1 and a cytidine-specific recombinant RNase Cusativin.
    Type: Grant
    Filed: November 12, 2019
    Date of Patent: December 27, 2022
    Assignee: University of Cincinnati
    Inventors: Sarah Venus, Balasubrahmanyam Addepalli, Nicholas Paul Lesner, Patrick Alan Limbach
  • Compositions, methods, and kits for one-step digestion of nucleic acid for analysis by liquid chromatography tandem mass spectrometry
    Patent number: 11441170
    Abstract: A kit for preparing a nucleic acid sample for analysis by liquid chromatography tandem mass spectrometry (LC-MS/MS) is provided, the kit comprising: a lyophilized enzyme composition comprising: micrococcal nuclease; nuclease P1; and bacterial alkaline phosphatase (BAP); and a digestion buffer. Also provided are enzyme compositions and methods of use for rapid, efficient preparation of a nucleic acid sample for analysis by LC-MS/MS, without the need for denaturation of the sample.
    Type: Grant
    Filed: October 26, 2018
    Date of Patent: September 13, 2022
    Assignee: University of Cincinnati
    Inventors: Robert Ross, Patrick A. Limbach, Balasubrahmanyam Addepalli, Manasses Jora
  • Compositions, Methods, and Kits for One-Step Digestion of Nucleic Acid for Analysis by Liquid Chromatography Tandem Mass Spectrometry
    Publication number: 20200255886
    Abstract: A kit for preparing a nucleic acid sample for analysis by liquid chromatography tandem mass spectrometry (LC-MS/MS) is provided, the kit comprising: a lyophilized enzyme composition comprising: micrococcal nuclease; nuclease P1; and bacterial alkaline phosphatase (BAP); and a digestion buffer. Also provided are enzyme compositions and methods of use for rapid, efficient preparation of a nucleic acid sample for analysis by LC-MS/MS, without the need for denaturation of the sample.
    Type: Application
    Filed: October 26, 2018
    Publication date: August 13, 2020
    Inventors: Robert Ross, Patrick A. Limbach, Balasubrahmanyam Addepalli, Manasses Jora
  • RECOMBINANT NUCLEOSIDE-SPECIFIC RIBONUCLEASE AND METHOD OF PRODUCING AND USING SAME
    Publication number: 20200115689
    Abstract: A recombinant ribonuclease is disclosed. The recombinant ribonuclease is produced by introducing a recombinant DNA sequence into a host; activating expression of the recombinant DNA sequence within the host to produce the recombinant ribonuclease; and isolating the recombinant ribonuclease from the host. Additionally, a method of analyzing an RNA sequence includes digesting the RNA with a first recombinant ribonuclease to give digestion products comprising nucleotides of the RNA sequence; and analyzing the digestion products using an analytical method to provide the identity of at least some of the nucleotides. The recombinant ribonuclease includes at least one of a uridine-specific recombinant RNase MC1 and a cytidine-specific recombinant RNase Cusativin.
    Type: Application
    Filed: November 12, 2019
    Publication date: April 16, 2020
    Inventors: Sarah Venus, Balasubrahmanyam Addepalli, Nicholas Paul Lesner, Patrick Alan Limbach
  • Recombinant nucleoside-specific ribonuclease and method of producing and using same
    Patent number: 10533163
    Abstract: A recombinant ribonuclease is disclosed. The recombinant ribonuclease is produced by introducing a recombinant DNA sequence into a host; activating expression of the recombinant DNA sequence within the host to produce the recombinant ribonuclease; and isolating the recombinant ribonuclease from the host. Additionally, a method of analyzing an RNA sequence includes digesting the RNA with a first recombinant ribonuclease to give digestion products comprising nucleotides of the RNA sequence; and analyzing the digestion products using an analytical method to provide the identity of at least some of the nucleotides. The recombinant ribonuclease includes at least one of a uridine-specific recombinant RNase MC1 and a cytidine-specific recombinant RNase Cusativin.
    Type: Grant
    Filed: April 25, 2016
    Date of Patent: January 14, 2020
    Assignee: University of Cincinnati
    Inventors: Sarah Venus, Balasubrahmanyam Addepalli, Nicholas Paul Lesner, Patrick Alan Limbach
  • RECOMBINANT NUCLEOSIDE-SPECIFIC RIBONUCLEASE AND METHOD OF PRODUCING AND USING SAME
    Publication number: 20180320154
    Abstract: A recombinant ribonuclease is disclosed. The recombinant ribonuclease is produced by introducing a recombinant DNA sequence into a host; activating expression of the recombinant DNA sequence within the host to produce the recombinant ribonuclease; and isolating the recombinant ribonuclease from the host. Additionally, a method of analyzing an RNA sequence includes digesting the RNA with a first recombinant ribonuclease to give digestion products comprising nucleotides of the RNA sequence; and analyzing the digestion products using an analytical method to provide the identity of at least some of the nucleotides. The recombinant ribonuclease includes at least one of a uridine-specific recombinant RNase MC1 and a cytidine-specific recombinant RNase Cusativin.
    Type: Application
    Filed: April 25, 2016
    Publication date: November 8, 2018
    Inventors: Sarah Venus, Balasubrahmanyam Addepalli, Nicholas Paul Lesner, Patrick Alan Limbach