Abstract: A gate valve body having a gate cavity intersecting a channel having a proximal channel portion and a distal channel portion on opposing sides of the gate cavity, a gate positioned in the gate cavity and moveable between closed and open positions, the gate having a proximal gate face and a distal gate face extending perpendicular to the channel; a proximal seat between a proximal valve body portion and the gate, the proximal seat having a first proximal seat face adjacent a proximal valve body face and a second proximal seat face adjacent the proximal gate face; a proximal annular groove on a radial outer surface of the proximal seat; a proximal axial seal positioned in the proximal annular groove contacting with the proximal seat and the valve body; and a proximal plurality of slots extending through the proximal seat between the proximal channel portion and the proximal annular groove.

Abstract: The present invention relates to a method for modifying the glycosylation profile of a recombinant glycoprotein produced in cell culture comprising culturing eukaryotic cells expressing the recombinant glycoprotein in a cell culture medium, wherein the cell culture medium is supplemented with fucose, manganese, and taurine, wherein the glycosylation profile of the produced recombinant glycoprotein is modified to better resemble the glycosylation profile of a reference glycoprotein than when cultured without said supplementation.

Abstract: A gate valve body having a gate cavity intersecting a channel having a proximal channel portion and a distal channel portion on opposing sides of the gate cavity, a gate positioned in the gate cavity and moveable between closed and open positions, the gate having a proximal gate face and a distal gate face extending perpendicular to the channel; a proximal seat between a proximal valve body portion and the gate, the proximal seat having a first proximal seat face adjacent a proximal valve body face and a second proximal seat face adjacent the proximal gate face; a proximal annular groove on a radial outer surface of the proximal seat; a proximal axial seal positioned in the proximal annular groove in contact with the proximal seat and the valve body; and a proximal conduit extending through the proximal seat between the proximal channel portion and the proximal annular groove.

Abstract: An internal locking ring assembly for a wellhead system that includes a compact single body locking ring design that eliminates the need for a separate casing head and housing configuration or the use of numerous lockdown pins, therefore increasing the safety of the entire drilling operation. The internal locking ring includes outer and inner diameter grooves and angles to accommodate a running tool for collapsing the locking ring for positing into wellhead seats all at lower forces than what is typical due to the lower retrieving torque when trying to retrieve the packoff. Such lower retrieving torque and force allows the use of smaller cross sections of a running tool.

Abstract: A grease delivery with accumulated pressure system includes a grease accumulator comprising a first compartment containing a pressurized fluid and a second compartment containing a grease, a pressurized fluid source in communication with the first compartment, a grease reservoir connected to the second compartment through a supply conduit, and a discharge conduit extending from the second compartment to a receiving component.

Abstract: An exemplary gate valve includes a valve body having a gate cavity intersecting a through bore, the gate cavity extending through a first end of the valve body, an inner surface forming the gate cavity is angled outward from a center axis of the gate cavity to a gate cavity opening at the first end, and a gate positioned in the gate cavity and moveable between a closed position blocking flow through the through bore and an open position allowing flow through the through bore, the gate having a sealing face extending perpendicular to the through bore.

Abstract: The present invention relates to a method for modifying the glycosylation profile of a recombinant glycoprotein produced in cell culture comprising culturing eukaryotic cells expressing the recombinant glycoprotein in a cell culture medium, wherein the cell culture medium is supplemented with fucose, manganese, and taurine, wherein the glycosylation profile of the produced recombinant glycoprotein is modified to better resemble the glycosylation profile of a reference glycoprotein than when cultured without said supplementation.

Abstract: A gate valve system for controlling fluid flow having a valve body with a bore through which fluid can flow. The system may include a hydra seal assembly for forming a tight seal against the gate of the valve. A programmable logic controller (PLC) may further control the operation of the gate and the hydra seal assembly, and a frac stack may be remotely and automatically controlled for remote operation.

Abstract: A gate valve system for controlling fluid flow having a valve body with a bore through which fluid can flow. The system includes a valve body having a bore to allow fluid to pass through the valve body and further having a cavity defined by substantially parallel innerside walls of the valve body, a gate wherein at least one end of the gate is defined by a radius perimeter. The system also has a bonnet that is removably coupled to a face of the valve body, wherein a portion of an innerside of the bonnet includes a curved surface adapted to mate with the at least one end of the gate defined by the radius perimeter, and an actuation mechanism operationally positioned and removably connected to the gate by way of a thru-hole in the bonnet.

Abstract: A gate valve system for controlling fluid flow having a valve body with a bore through which fluid can flow. The system includes a valve body having a bore to allow fluid to pass through the valve body and further having a cavity defined by substantially parallel innerside walls of the valve body, a gate wherein at least one end of the gate is defined by a radius perimeter. The system also has a bonnet that is removably coupled to a face of the valve body, wherein a portion of an innerside of the bonnet includes a curved surface adapted to mate with the at least one end of the gate defined by the radius perimeter, and an actuation mechanism operationally positioned and removably connected to the gate by way of a thru-hole in the bonnet.