Abstract: A grease delivery with accumulated pressure system includes a grease accumulator comprising a first compartment containing a pressurized fluid and a second compartment containing a grease, a pressurized fluid source in communication with the first compartment, a grease reservoir connected to the second compartment through a supply conduit, and a discharge conduit extending from the second compartment to a receiving component.

Abstract: ABC multidrug transporters are key players in cancer multidrug resistance and in general xenobiotic elimination, thus their functional assays provide important tools for research and diagnostic applications. It has been found that in cells expressing functional ABCG2, ABCB1, or ABCC1 transporters, cellular PG fluorescence is strongly reduced. The invention relates to methods and uses of fluorescein derivative ester compounds of formula Ia which are analogs of PG for assessing ABC transporter activity of ABC multidrug transporters. The present accumulation assay is a novel tool for the parallel determination of the function of the multidrug transporters, in particular ABCG2, ABCB1, and ABCC1. The assay is applicable for diagnostic purposes and also allows the selection, separation and culturing of selected cell populations expressing such transporters.

Abstract: An exemplary gate valve includes a valve body having a gate cavity intersecting a through bore, the gate cavity extending through a first end of the valve body, an inner surface forming the gate cavity is angled outward from a center axis of the gate cavity to a gate cavity opening at the first end, and a gate positioned in the gate cavity and moveable between a closed position blocking flow through the through bore and an open position allowing flow through the through bore, the gate having a sealing face extending perpendicular to the through bore.

Abstract: The present invention relates to a method for modifying the glycosylation profile of a recombinant glycoprotein produced in cell culture comprising culturing eukaryotic cells expressing the recombinant glycoprotein in a cell culture medium, wherein the cell culture medium is supplemented with fucose, manganese, and taurine, wherein the glycosylation profile of the produced recombinant glycoprotein is modified to better resemble the glycosylation profile of a reference glycoprotein than when cultured without said supplementation.

Abstract: A gate valve system for controlling fluid flow having a valve body with a bore through which fluid can flow. The system may include a hydra seal assembly for forming a tight seal against the gate of the valve. A programmable logic controller (PLC) may further control the operation of the gate and the hydra seal assembly, and a frac stack may be remotely and automatically controlled for remote operation.

Abstract: A gate valve system for controlling fluid flow having a valve body with a bore through which fluid can flow. The system includes a valve body having a bore to allow fluid to pass through the valve body and further having a cavity defined by substantially parallel innerside walls of the valve body, a gate wherein at least one end of the gate is defined by a radius perimeter. The system also has a bonnet that is removably coupled to a face of the valve body, wherein a portion of an innerside of the bonnet includes a curved surface adapted to mate with the at least one end of the gate defined by the radius perimeter, and an actuation mechanism operationally positioned and removably connected to the gate by way of a thru-hole in the bonnet.

Abstract: A gate valve system for controlling fluid flow having a valve body with a bore through which fluid can flow. The system includes a valve body having a bore to allow fluid to pass through the valve body and further having a cavity defined by substantially parallel innerside walls of the valve body, a gate wherein at least one end of the gate is defined by a radius perimeter. The system also has a bonnet that is removably coupled to a face of the valve body, wherein a portion of an innerside of the bonnet includes a curved surface adapted to mate with the at least one end of the gate defined by the radius perimeter, and an actuation mechanism operationally positioned and removably connected to the gate by way of a thru-hole in the bonnet.