Patents by Inventor Balazs Siminszky
Balazs Siminszky has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9228195Abstract: Compositions and methods for reducing the level of nornicotine and N?-nitrosonornicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.Type: GrantFiled: January 30, 2012Date of Patent: January 5, 2016Assignees: North Carolina State University, University of Kentucky Research FoundationInventors: Ralph E. Dewey, Balazs Siminszky, Steven W. Bowen, Lily Gavilano
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Patent number: 9187759Abstract: Compositions and methods for reducing the level of nornicotine and N?-nitrosonornicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.Type: GrantFiled: December 17, 2010Date of Patent: November 17, 2015Assignees: North Carolina State University, University of Kentucky Research FoundationInventors: Ralph E. Dewey, Balazs Siminszky, Steven W. Bowen, Lily Gavilano
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Publication number: 20140190499Abstract: In one aspect, there is provided a method for reducing the formation of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone and N-nitrosonornicotine and N-nitrosoanatabine and N-nitrosoanabasine in a tobacco homogenate comprising the steps of: (a) providing a tobacco homogenate; (b) increasing the pH of the tobacco homogenate to at least about pH 6.0; (c) optionally measuring the concentration of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone and N-nitrosonornicotine and N-nitrosoanatabine and N-nitrosoanabasine in the tobacco homogenate before and after the pH treatment in step (b); and (d) obtaining a tobacco homogenate in which the levels of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone and N-nitrosonornicotine and N-nitrosoanatabine and N-nitrosoanabasine are reduced as compared to the levels of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone and N-nitrosonornicotine and N-nitrosoanatabine and N-nitrosoanabasine in the tobacco homogenate provided in step (a).Type: ApplicationFiled: May 24, 2012Publication date: July 10, 2014Applicant: PHILIP MORRIS PRODUCTS S.A.Inventor: Balazs Siminszky
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Publication number: 20120117933Abstract: Compositions and methods for reducing the level of nornicotine and N?-nitrosonornicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.Type: ApplicationFiled: January 30, 2012Publication date: May 17, 2012Applicants: University of Kentucky Research Foundation, North Carolina State UniversityInventors: Ralph E. Dewey, Balazs Siminszky, Steven W. Bowen, Lily Gavilano
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Publication number: 20120118308Abstract: Compositions and methods for reducing the level of nornicotine and N?-nitrosonornicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.Type: ApplicationFiled: January 30, 2012Publication date: May 17, 2012Applicants: University of Kentucky Research Foundation, North Carolina State UniversityInventors: Ralph E. Dewey, Balazs Siminszky, Steven W. Bowen, Lily Gavilano
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Patent number: 8124851Abstract: Compositions and methods for reducing the level of nornicotine and N?-nitrosonornicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.Type: GrantFiled: November 12, 2008Date of Patent: February 28, 2012Assignees: North Carolina State University, University of Kentucky Research FoundationInventors: Ralph E. Dewey, Balazs Siminszky, Steven W. Bowen, Lily Gavilano
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Publication number: 20110174322Abstract: Compositions and methods for reducing the level of nornicotine and N?-nitrosonornicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.Type: ApplicationFiled: December 17, 2010Publication date: July 21, 2011Applicants: North Carolina State University, University of Kentucky Research FoundationInventors: Ralph E. Dewey, Steven W. Bowen, Balazs Siminszky, Lily Gavilano
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Patent number: 7884263Abstract: Compositions and methods for reducing the level of nornicotine and N?-nitrosonornicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.Type: GrantFiled: October 13, 2006Date of Patent: February 8, 2011Assignees: North Carolina State University, University of Kentucky Research FoundationInventors: Ralph E. Dewey, Steven W. Bowen, Balazs Siminszky, Lily Gavilano
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Publication number: 20090205072Abstract: Compositions and methods for reducing the level of nornicotine and N?-nitrosonornicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.Type: ApplicationFiled: November 12, 2008Publication date: August 13, 2009Applicants: North Carolina State University, University of Kentucky Research FoundationInventors: Ralph E. Dewey, Balazs Siminszky, Steven W. Bowen, Lily Gavilano
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Publication number: 20080202541Abstract: Compositions and methods for reducing the level of nornicotine and N?-nitrosonornicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.Type: ApplicationFiled: October 13, 2006Publication date: August 28, 2008Applicants: North Carolina State University, University of Kentucky Research FoundationInventors: Ralph E. Dewey, Steven W. Bowen, Balazs Siminszky, Lily Gavilano
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Patent number: 6121512Abstract: DNA sequence encoding novel cytochrome P-450 molecules are provided. The use of DNA constructs containing such molecules to transform plants is described, as are transgenic plants exhibiting increased resistance to phenylurea herbicides. Methods of using such DNA constructs and transformed plants are provided.Type: GrantFiled: October 10, 1997Date of Patent: September 19, 2000Assignee: North Carolina State UniversityInventors: Balazs Siminszky, Ralph Dewey, Frederick Corbin