Abstract: The present invention provides methods for targeted delivery of agents (e.g., drugs) to cells (e.g., cancer cells) using agent-polymer conjugates and bispecific targeting molecules. The invention further provides compositions and kits for practicing the targeted delivery methods.

Abstract: The present invention provides methods for targeted delivery of agents (e.g., drugs) to cells (e.g., cancer cells) using agent-polymer conjugates and bispecific targeting molecules. The invention further provides compositions and kits for practicing the targeted delivery methods.

Abstract: The invention provides methods for delivery of payloads to targets in samples using non-sterically hindered complexes. The invention further provides reagents and kits for practicing the methods of the invention. The invention also provides methods for preparation of reagents for use in the methods of the invention.

Abstract: Methods of making bispecific binding complexes and nanopolymers coupled to detection and/or therapeutic agents are disclosed. Also disclosed are methods of using such bispecific binding complexes and nanopolymers for detecting and treating cells.

Abstract: An immunoassay method including reacting a sample from a patient with a bispecific antibody, wherein the bispecific antibody includes one antibody specific for a compound to be detected and a second antibody specific for a compound foreign to said patient sample, and subsequently reacting the patient sample with a polymer probe, wherein the polymer probe includes a compound recognized by the second antibody in the bispecific antibody complex and further includes at least two detectable signals; the bispecific antibody; and the polymer probe of the immunoassay method are disclosed.

Abstract: An immunoassay method including reacting a sample from a patient with a bispecific antibody, wherein the bispecific antibody includes one antibody specific for a compound to be detected and a second antibody specific for a compound foreign to said patient sample, and subsequently reacting the patient sample with a polymer probe, wherein the polymer probe includes a compound recognized by the second antibody in the bispecific antibody complex and further includes at least two detectable signals; the bispecific antibody; and the polymer probe of the immunoassay method are disclosed.

Abstract: An immunoassay method including reacting a sample from a patient with a bispecific antibody, wherein the bispecific antibody includes one antibody specific for a compound to be detected and a second antibody specific for a compound foreign to said patient sample, and subsequently reacting the patient sample with a polymer probe, wherein the polymer probe includes a compound recognized by the second antibody in the bispecific antibody complex and further includes at least two detectable signals; the bispecific antibody; and the polymer probe of the immunoassay method are disclosed.

Abstract: The present invention relates to compositions and methods for detecting very low concentrations of a molecule in a mixture. The detection of the molecule comprises the steps of first contacting a sample with a multispecific molecule capable of binding at least two molecules including the molecule to be detected, wherein the molecule to be detected is bound by the multispecific molecule thereby forming a complex, and second contacting the complex with a second, different molecule which is linked via a polymer to multiple detection signaling molecules. The invention may also be practiced by administration of the multispecific molecule in vivo, to a host for the molecule to be detected, either with or without the bound polymer probe and thereafter, respectively, either detecting the signaling molecule on the probe, or administering the probe and allowing it to bind the multispecific molecule, followed by detection of the signaling molecule on the probe.

Abstract: A method is provided for inducing tolerance to T-cell mediated immunity. Particularly, tolerance is induced in a recipient mammal to a transplanted organ or tissue. Tolerance is induced by treating the recipient mammal with a MHC antigen which has been modified to render an immunosuppressive effect.

Abstract: The invention relates to methods of salvaging a target cell from cell death, comprising contacting a target cell having a disrupted cell membrane with a specific affinity reagent-liposome conjugate in an amount effective and for a time sufficient to allow the conjugate to prevent cell death due to membrane disruption; and determining the viability of the target cell. Methods of delivering a selected agent into a damaged target cell for diagnosis and therapy are also disclosed.

Abstract: The invention relates to a method of detecting an Fc receptor-expressing tumor site in an individual by administering to the individual a diagnostically effective amount of detectably-labeled non-specific immunoglobulin or Fc or Fc' fragment thereof, wherein the immunoprotein substantially accumulates at the site when the site bears such a tumor. By using a therapeutically-labeled specific or non-specific immunoglobulin or Fc or Fc' fragment thereof, the tumor may be treated therapeutically.

Abstract: An in vivo imaging or therapeutic method including administering to a mammal a specific affinity reagent including a physiologically compatible polymer bonded to one or more specific affinity molecules capable of selectively binding to a predetermined substance or class of cells, and one or more reporter groups capable of providing detectable contrast between the predetermined substance or class of cells and cells or substances of the mammal to which the specific affinity reagent does not bind, or of killing cells of the predetermined class.

Abstract: The invention relates to a method of detecting early rejection of an allograft in a mammal by administering to the mammal a diagnostically effective amount of detectably labeled immunoglobulin or fragment thereof, wherein the immunoglobulin substantially accumulates at the site of allograft rejection. The method of the invention may also be used to monitor allograft rejection during immunosuppressive therapy in a mammal which has received an allograft or to localize areas involved in autoimmune disease or inflammation.

Abstract: The invention relates to a method of detecting an Fc receptor-expressing tumor site in an individual by administering to the individual a diagnostically effective amount of detectably-labeled non-specific immunoglobulin or Fc or Fc' fragment thereof, wherein the immunoprotein substantially accumulates at the site when the site bears such a tumor. By using a therapeutically-labeled specific or non-specific immunoglobulin or Fc or Fc' fragment thereof, the tumor may be treated therapeutically.

Abstract: Method using technetium-99m imaging agents for the study, detection or diagnosis of ischemic, infarcted or diseased tissue. The imaging agents comprise a complex of technetium-99 m and glucarate.

Abstract: Protein molecules are covalently bonded to a chelating agent which in turn binds a radioactive molecule. Prior to binding, the radioactive molecule is reduced with dithionite ion and then mixed with protein at a pH of 7.0 to about 8.0.