Abstract: The current disclosure pertains to Type II Cas proteins, CRISPR-Cas systems, and their various applications. The Type II Cas proteins outlined in this disclosure broaden the utility of CRISPR-Cas systems for targeting or modifying genes.

Abstract: This invention provides compounds and processes that allow enzymes to catalyze the copying of DNA and RNA molecules to make complements where individual nucleotides are replaced in a rule-based fashion by other nucleotides, using biversal nucleotides that pair with two different nucleotides.

Abstract: The present disclosure relates to enriching nucleic acid from a sample. In some embodiments, the present disclosure provides methods for enriching at least one targeted nucleic acid, identifying genome-wide gene editing off-targets, and evaluating gene editing efficiency from a sample comprising a plurality of single-strand nucleic acid fragments. Others example embodiments are also described herein.

Abstract: The present disclosure relates to enriching nucleic acid from a sample. In some embodiments, the present disclosure provides methods for enriching at least one targeted nucleic acid, identifying genome-wide gene editing off-targets, and evaluating gene editing efficiency from a sample comprising a plurality of single-strand nucleic acid fragments. Others example embodiments are also described herein.

Abstract: Disclosed herein are systems comprising one or more modified single-guide RNAs (sgRNAs) and a donor DNA, wherein each of the modified sgRNAs comprises one or more internal anchors that are at least 5 nucleotides away from both 3? and 5? ends of each of the modified sgRNAs, wherein the donor DNA comprises one or more binding segments capable of binding to an internal anchor of the one or more internal anchors.

Abstract: A dust collecting attachment includes a dust suction portion and a main body. The dust suction portion has a suction opening through which a tip tool is allowed to pass. The main body has a dust collection passage communicated with the dust suction portion and connectable to an external hose. The main body includes a suction space, an air vent hole, and an air vent operation portion. The suction space is communicated with the dust collection passage and has a suction port opening to a workpiece. The air vent hole opens the suction space to outside. The air vent operation portion normally closes the air vent hole and opens the air vent hole by a predetermined operation. The air vent operation portion is operated together with an operation of removing the main body from the workpiece to open the air vent hole.

Abstract: A dust collecting attachment includes a dust suction portion and a main body. The main body has a dust collection passage communicated with the dust suction portion. The dust suction portion has a negative pressure by exerting a suction force to the dust collection passage from a hose. The dust suction portion includes an inner pipe through which a tip tool passes, a first dust suction flow passage, and a second dust suction flow passage. The first dust suction flow passage opens a first suction opening to a side of a workpiece in an outside in a radial direction of the inner pipe and is communicated with the dust collection passage. The second dust suction flow passage opens a second suction opening to an opposite side of the workpiece in the outside in the radial direction of the inner pipe and is communicated with the dust collection passage.

Abstract: The present disclosure relates to enriching nucleic acid from a sample. In some embodiments, the present disclosure provides methods for enriching at least one targeted nucleic acid, identifying genome-wide gene editing off-targets, and evaluating gene editing efficiency from a sample comprising a plurality of single-strand nucleic acid fragments. Others example embodiments are also described herein.

Abstract: A sample preparation mould and a sample preparation method for the triaxial test of MSW are provided. In the present invention, the cylindrical main mould comprises two curved steel pieces, and the two sides of each curved steel piece have a stretched connection part; the two curved steel pieces are connected with each other and clamped by two steel clamps; porous stones are arranged at two ends of the cylindrical main mould and fixed separately by a top limiting lantern ring and a bottom lantern ring; the top limiting lantern ring is detachably connected to the top hopper, from which MSW materials are input; the cylindrical main mould is radially constrained in four positions, and the two ends thereof are axially constrained, thereby significantly improving stability during compacting and hence ensuring the axial and radial sizes of the MSW sample.

Abstract: The lack of blood-brain barrier (BBB) penetrating ability has hindered the delivery of many therapeutic agents for tauopathy therapeutic treatment. A circular bifunctional aptamer reported here has been able to enhance the in vivo BBB penetration for improved therapy. The circular aptamer includes one transferrin receptor (TfR) aptamer to facilitate TfR-aptamer recognition-induced transcytosis across BBB endothelial cells, and one Tau protein aptamer selected to inhibit Tau phosphorylation and other tauopathy-related pathological events in the brain. This bispecific construct exhibits strong specificity towards Tau and enhanced plasma stability in comparison to linear Tau aptamer. In vivo administration of circular Tau-TfR aptamer results in a rapid uptake into relevant brain regions after crossing the BBB, such as hippocampus and cortex.

Abstract: The present disclosure provides a data processing method. The data processing method includes acquiring a first original image and performing a first processing on the first original image to obtain a first processed image, a resolution parameter of the first processed image and the resolution parameter of the first original image having a first difference; and acquiring a second original image and performing a second processing on the second original image to obtain a second processed image. The resolution parameter of the second processed image and the resolution parameter of the second original image have a second difference. The second difference is different from the first difference. The first original image and the second original image are two frames in a plurality of consecutively acquired original images.

Abstract: A sample preparation mould and a sample preparation method for the triaxial test of MSW are provided. In the present invention, the cylindrical main mould comprises two curved steel pieces, and the two sides of each curved steel piece have a stretched connection part; the two curved steel pieces are connected with each other and clamped by two steel clamps; porous stones are arranged at two ends of the cylindrical main mould and fixed separately by a top limiting lantern ring and a bottom lantern ring; the top limiting lantern ring is detachably connected to the top hopper, from which MSW materials are input; the cylindrical main mould is radially constrained in four positions, and the two ends thereof are axially constrained, thereby significantly improving stability during compacting and hence ensuring the axial and radial sizes of the MSW sample.

Abstract: The present disclosure provides a data processing method. The data processing method includes acquiring a first original image and performing a first processing on the first original image to obtain a first processed image, a resolution parameter of the first processed image and the resolution parameter of the first original image having a first difference; and acquiring a second original image and performing a second processing on the second original image to obtain a second processed image. The resolution parameter of the second processed image and the resolution parameter of the second original image have a second difference. The second difference is different from the first difference. The first original image and the second original image are two frames in a plurality of consecutively acquired original images.

Abstract: Disclosed is a gap support measuring method which performs autonomous gap allocation without lowering the data transfer rate or the throughput. ST301 acquires various kinds of parameters decided by a network so as to execute a gap support measurement task. ST302 checks the current time and starts the gap support measurement task when the current time has become Ts. ST303 judges whether CQI measured by a terminal is lower than a threshold value. ST304 judges whether the threshold value should be updated. ST306 decides the gap length according to the remaining gap length and the remaining time and performs measurement within the gap. ST307 judges whether the measurement is complete. ST308 checks whether the total of the gaps exceed the entire gap length Tg.

Abstract: Disclosed is a gap support measuring method which performs autonomous gap allocation without lowering the data transfer rate or the throughput. ST301 acquires various kinds of parameters decided by a network so as to execute a gap support measurement task. ST302 checks the current time and starts the gap support measurement task when the current time has become Ts. ST303 judges whether CQI measured by a terminal is lower than a threshold value. ST304 judges whether the threshold value should be updated. ST306 decides the gap length according to the remaining gap length and the remaining time and performs measurement within the gap. ST307 judges whether the measurement is complete. ST308 checks whether the total of the gaps exceed the entire gap length Tg.

Abstract: An electronic apparatus and a heat-dissipation device thereof. The electronic apparatus includes a plate and the heat-dissipation device. The plate includes a first component and a second component thereon. The heat-dissipation device is adjacent to the plate, and includes a first sensor, a first cooling component, a second sensor, and a second cooling component thereon. The first sensor measures the temperature of the plate around the first component. The first cooling component is coupled to the first sensor to adjust the temperature of the plate around the first component. The second sensor measures the temperature of the plate around the second component. The second cooling component is coupled to the second sensor to adjust the temperature of the plate around the second component.