Abstract: An anti-pollution test device and a method for rapid test of nucleic acid amplifications, and a use. The anti-pollution test device includes a test portion and a mixing reaction chamber, where the mixing reaction chamber includes: a reaction chamber body, a reaction chamber cover, a channel and a mixing reaction portion. The test portion includes a test inner core, a test kit, a transparent observation window, a flow guide device, and the flow guide device including: a flow guide tube and a flow guide component. One end of the flow guide component is connected to the flow guide tube, one end of the flow guide component is connected to the test inner core, to guide a mixed solution of a hybridization solution and a sample to the test inner core. The test inner core displays a final test result in an area corresponding to the transparent observation window.