Abstract: The disclosure provides a method for preparing a bacterial cellulose membrane using an enzymatic soybean hydrolysate, comprising the following steps: (1) preparation of an enzymatic soybean hydrolysate medium; (2) preparation of a crude bacterial cellulose membrane; and (3) purification of a bacterial cellulose membrane. The method of the disclosure rationally uses the residual waste liquid obtained from enzymatic preparation of soybean oil, and without acid hydrolysis treatment of a medium, the bacterial cellulose synthesized by bacterial strains directly using an enzymatic soybean hydrolysate has a greater amount, finer and denser microfibers, and a higher maximum thermal degradation temperature.

Abstract: The present invention provides a method for aqueous enzymatic extraction of soybean oil, which is in the field of extraction processing technology for plant oil. The method comprises treating crushed and peeled soybeans with an extrusion puffing process and a hydrolysis process with an alkaline proteinase, followed by a liquid nitrogen freezing and a high voltage electrostatic thawing process, and finally obtaining soybean oil by centrifugation. The benefits of the present invention includes short extraction time and high extraction yield. It saves up to 93.1% of time in the freezing and thawing process alone. The total oil yield is up to 95.4%. Furthermore, the method of the invention produces high quality oil with a low peroxide value, a low p-anisidine value and a low TOTOX value.