Abstract: Disclosed is a detection device for a working surface. The detection device (100) comprises: a plurality of hydraulic supports (101), a laser ranging set (102), a displacement sensor (103), a hydroelectric signal conversion module (104), and a support controller (105) which are disposed on a working surface. The laser ranging set (102) is disposed below a top beam of a first target hydraulic support among the plurality of hydraulic supports and parallel to a post of the first target hydraulic support, and is configured to determine error length and send the error length to the hydroelectric signal conversion module (104). The displacement sensor (103) is configured to at least obtain the degrees of inclination of the hydraulic supports (101) and send the degrees of inclination to the hydroelectric signal conversion module (104).

Abstract: Provided are amino acid sequences of engineered transaminase polynucleotide that are useful for asymmetrically synthesizing chiral amine compounds, and its preparation process and reaction process under industrial-relevant conditions. Also provided are polynucleotide sequences encoding engineered transaminase polypeptides, engineered host cells capable of expressing engineered transaminase polypeptides, and methods of producing chiral amine compounds using engineered transaminase polypeptides. Compared to other enzymes, the engineered transaminase polypeptides provided by this invention have better catalytic activity and stability, and are not inhibited by chiral amine products in the synthesis process. The use of the engineered polypeptides of the present invention for the preparation of chiral amine compound results in higher unit activity, and has good industrial application prospects.