Abstract: The present invention provides stem cells-derived extracellular vesicles (EVs) characterized by upregulation and/or downregulation of specific proteins. Composition comprising the EVs. and their use in the treatment of diseases and disorders associated with the upregulated proteins are also provided.

Abstract: The present invention provides methods and systems for enhanced production and/or secretion of extracellular vesicles from at least one three-dimensional porous scaffold having a population of stem cells cultured thereon, utilizing various shear stress conditions on a variety of stem cells.

Abstract: A flow bioreactor device for monitoring cellular dynamics may include a housing having one or a plurality of conduits to place one or a plurality of tissue samples inside each of said one or a plurality of conduits, wherein each of said one or a plurality of conduits has an inlet for introducing a flow of a liquid in a direction of flow through that conduit, wherein said one or a plurality of conduits are fluidically linked to an outlet for discharging the liquid, and wherein at least a portion of the housing is transparent so as to provide a line of sight for viewing or for an optical device along an elongated axis of any of said one or a plurality of conduits, the line of sight being substantially parallel to the direction of flow through that conduit.

Abstract: Methods for using vibration to harvest cells grown in 3D culture are provided. The methods entail the application of force cells attached to a 3D matrix of sufficient amplitude, frequency, and duration to detach cells from the matrix and to flush the detached cells out of the matrix material. An apparatus for performing the methods of the invention as provided.

Abstract: Methods for using vibration to harvest cells grown in 3D culture are provided. The methods entail the application of force cells attached to a 3D matrix of sufficient amplitude, frequency, and duration to detach cells from the matrix and to flush the detached cells out of the matrix material. An apparatus for performing the methods of the invention as provided.

Abstract: Methods for using vibration to harvest cells grown in 3D culture are provided. The methods entail the application of force to cells attached to a 3D matrix of sufficient amplitude, frequency, and duration to detach cells from the matrix and to flush the detached cells out of the matrix material. An apparatus for performing the methods of the invention as provided.