Patents by Inventor Barbara A. Moffatt

Barbara A. Moffatt has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Cloning and expression of the gene for bacteriophage T7 RNA polymerase
    Patent number: 5869320
    Abstract: This application describes a means to clone a functional gene for bacteriophage T7 RNA polymerase. Active T7 RNA polymerase is produced from the cloned gene, and a plasmid has been constructed that can produce the active enzyme in large amounts. T7 RNA polymerase transcribes DNA very efficiently and is highly selective for a relatively long promoter sequence. This enzyme is useful for synthesizing large amounts of RNA in vivo or in vitro, and is capable of producing a single RNA selectively from a complex mixture of DNAs. The procedure used to obtain a clone of the R7 RNA polymerase gene can be applied to other T7-like phages to obtain clones that produce RNA polymerases having different promoter specificities, different bacterial hosts, or other desirable properties. T7 RNA polymerase is also used in a system for selective, high-level synthesis of RNAs and proteins in suitable host cells.
    Type: Grant
    Filed: January 15, 1997
    Date of Patent: February 9, 1999
    Assignee: Brookhaven Science Associates LLC
    Inventors: F. William Studier, Parichehre Davanloo, Alan H. Rosenberg, Barbara A. Moffatt, John J. Dunn
  • Gene for APRT from plant tissue
    Patent number: 5770718
    Abstract: A cDNA for apt from a plant, particularly A. thaliana has been isolated, purified and characterized. The cDNA is useful for the generation of APRT-deficient mutants and as a probe for APRT cDNAs in other plants.
    Type: Grant
    Filed: May 22, 1995
    Date of Patent: June 23, 1998
    Assignee: University of Waterloo
    Inventor: Barbara Moffatt
  • Cloning and expression of the gene for bacteriophage T7 RNA polymerase
    Patent number: 5693489
    Abstract: This application describes a means to clone a functional gene for bacteriophage T7 RNA polymerase. Active T7 RNA polymerase is produced from the cloned gene, and a plasmid has been constructed that can produce the active enzyme in large amounts. T7 RNA polymerase transcribes DNA very efficiently and is highly selective for a relatively long promoter sequence. This enzyme is useful for synthesizing large amounts of RNA in vivo or in vitro, and is capable of producing a single RNA selectively from a complex mixture of DNAs. The procedure used to obtain a clone of the R7 RNA polymerase gene can be applied to other T7-like phages to obtain clones that produce RNA polymerases having different promoter specificities, different bacterial hosts, or other desirable properties. T7 RNA polymerase is also used in a system for selective, high-level synthesis of RNAs and proteins in suitable host cells.
    Type: Grant
    Filed: June 14, 1994
    Date of Patent: December 2, 1997
    Assignee: Associated Universities, Inc.
    Inventors: F. William Studier, Parichehre Davanloo, Alan H. Rosenberg, Barbara A. Moffatt, John J. Dunn
  • Cloning and expression of the gene for bacteriophage T7 RNA polymerase
    Patent number: 4952496
    Abstract: This application describes a means to clone a functional gene for bacteriophage T7 RNA polymerase. Active T7 RNA polymerase is produced from the cloned gene, and a plasmid has been constructed that can produce the active enzyme in large amounts. T7 RNA polymerase transcribes DNA very efficiently and is highly selective for a relatively long promoter sequence. This enzyme is useful for synthesizing large amounts of RNA in vivo or in vitro, and is capable of producing a single RNA selectively from a complex mixture of DNAs. The procedure used to obtain a clone of the T7 RNA polymerase gene can be applied to other T7-like phages to obtain clones that produce RNA polymerases having different promoter specificities, different bacterial hosts, or other desirable properties. T7 RNA polymerase is also used in a system for selective, high-level synthesis of RNAs and proteins in suitable host cells.
    Type: Grant
    Filed: December 29, 1986
    Date of Patent: August 28, 1990
    Assignee: Associated Universities, Inc.
    Inventors: F. William Studier, Parichehre Davanloo, Alan H. Rosenberg, Barbara A. Moffatt, John J. Dunn