Abstract: The present invention relates to nucleic acids encoding polypeptides involved in homologous recombination, as well as vectors and host cells comprising the nucleic acids and polypeptides encoded by the nucleic acids. Also provided are methods for inducing somatic and/or meiotic homologous recombination in a cell, comprising modulating the expression or properties of one or more gene products selected from the group consisting of AtIno80, At3g57300, Rvb1 (At5g22330), Rvb21 (At5g67630), Rvb22 (At3g49830), At3g57290, AtArp5.1 (At3g12380), AtArp5.2 (At5g56180), AtArp5.3 (At3g60830) and AtArp8 (At5g43500), their homologues, fragments or derivatives. In particular, the methods can be used to increase gene targeting.

Abstract: The invention provides methods for producing transgenic embryos and animals with a higher efficiency than presently available procedures based on Intracytoplasmic Sperm Injection (ICSI) of nucleic acid complexed with recombinase.

Abstract: A novel process for the direct transfer of foreign genes to plant genomes is described. The novel process comprises placing a gene under the control of plant expression signals and transferring it, by contact with protoplasts without the aid of natural systems for infecting plants, direct to plant cells from which genetically transformed plants can subsequently be derived.

Abstract: The present invention relates to a method for introducing nucleic acids into cells for e.g. producing transiently transfected or stably transformed animal cells by using a specifically designed nucleic acid/protein complex comprising in operable linkage to an expressible DNA or to an oligonucleotide a VirD2 protein, preferably together with a VirE2 protein.

Abstract: The present invention relates to a novel method of inserting viral DNA, which optionally may contain cargo-DNA, into plants or viable parts thereof, but preferably into plants of the monocotyledon class, and most preferably into plants of the family Gramineae, using suitable transfer microorganisms. Further comprised by the invention are recombinant DNA, plasmid and vector molecules suitably adapted to the specific conditions of the process according to the invention and the transgenic plant products obtainable in accordance with the said process.

Abstract: The present invention relates to a method for introducing nucleic acids into cells for e.g. producing transiently transfected or stably transformed animal cells by using a specifically designed nucleic acid/protein complex comprising in operable linkage to an expressible DNA or to an oligonucleotide a VirD2 protein, preferably together with a VirE2 protein.

Abstract: A novel process for the direct transfer of foreign genes to plant genomes is described. The novel process comprises placing a gene under the control of plant expression signals and transferring it, by contact with protoplasts without the aid of natural systems for infecting plants, direct to plant cells from which genetically transformed plants can subsequently be derived.

Abstract: A novel process for the direct transfer of foreign genes to plant genomes is described. The novel process comprises placing a gene under the control of plant expression signals and transferring it, by contact with protoplasts without the aid of natural systems for infecting plants, direct to plant cells from which genetically transformed plants can subsequently be derived.

Abstract: The present invention relates to a novel method of inserting viral DNA, which optionally may contain cargo-DNA, into plants or viable parts thereof, but preferably into plants of the monocotyledon class, and most preferably into plants of the family Gramineae, using suitable transfer microorganisms. Further comprised by the invention are recombinant DNA, plasmid and vector molecules suitably adapted to the specific conditions of the process according to the invention and the transgenic plant products obtainable in accordance with the said process.

Abstract: The present invention relates to a novel method of inserting viral DNA, which optionally may contain cargo-DNA, into plants or viable parts thereof, but preferably into plants of the monocotyledon class, and most preferably into plants of the family Gramineae, using suitable transfer microorganisms. Further comprised by the invention are recombinant DNA, plasmid and vector molecules suitably adapted to the specific conditions of the process according to the invention and the transgenic plant products obtainable in accordance with the said process.

Abstract: A novel process for the direct transfer of foreign genes to plant genomes is described. The novel process comprises placing a gene under the control of plant expression signals and transferring it, by contact with protoplasts without the aid of natural systems for infecting plants, direct to plant cells from which genetically transformed plants can subsequently be derived.

Abstract: A novel process for the direct transfer of foreign genes to plant genomes is described. The novel process comprises placing a gene under the control of plant expression signals and transferring it, by contact with protoplasts without the aid of natural systems for infecting plants, directly to plant cells from which genetically transformed plants can subsequently be derived.

Abstract: The present invention is directed to a process for the production of hybrid bacteria, characterized in that(a) a hybrid plasmid having only one cos site of a lambda or a lambdoid phage is produced from (1) a bacterial plasmid of not more than 21 Megadaltons and having only one cos site of a lambda or a lambdoid phage and (2) a foreign DNA fragment,(b) the resulting hybrid plasmid is packaged with the lysate of a lambda or lambdoid phage and(c) the packaging product is transduced into Escherichia coli whereby hybrid bacteria are formed.